PMID- 33979365
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20211028
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 5
DP  - 2021
TI  - Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES 
      cohort.
PG  - e0251259
LID - 10.1371/journal.pone.0251259 [doi]
LID - e0251259
AB  - MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a 
      variety of conditions and diseases, including complications during pregnancy. 
      During pregnancy, about 15-25% of maternal plasma exosomes, a small size-class of 
      EVs, are hypothesized to originate in the placenta, and may play a role in 
      communication between the fetus and mother. However, few studies have addressed 
      changes in miRNA over the course of pregnancy with repeated measures, nor focused 
      on diverse populations. We describe changes in miRNA in early and late pregnancy 
      from the MADRES cohort of primarily low-income Hispanic women based in Los 
      Angeles, CA. miRNA derived from extracellular-vesicles (EVs) were isolated from 
      maternal blood plasma samples collected in early and late pregnancy. In this 
      study, we identified 64 of 130 detectable miRNA which significantly increased 
      with gestational age at the time of collection (GA), and 26 which decreased with 
      GA. Possible fetal sex-specific associations were observed for 30 of these 90 
      significant miRNA. Predicted gene targets for miRNA significantly associated with 
      GA were identified using MirDIP and were found to be enriched for Gene Ontology 
      categories that included energetic and metabolic processes but were 
      underrepresented in immune-related categories. Circulating EV-associated miRNA 
      during pregnancy are likely important for maternal-fetal communication, and may 
      play roles in supporting and maintaining a healthy pregnancy, given the changing 
      needs of the fetus.
FAU - Foley, Helen Bermudez
AU  - Foley HB
AUID- ORCID: 0000-0002-1314-6813
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Howe, Caitlin G
AU  - Howe CG
AUID- ORCID: 0000-0002-4935-9298
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Eckel, Sandrah P
AU  - Eckel SP
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Chavez, Thomas
AU  - Chavez T
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Gevorkian, Lili
AU  - Gevorkian L
AUID- ORCID: 0000-0002-7588-6219
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Reyes, Eileen Granada
AU  - Reyes EG
AUID- ORCID: 0000-0002-4034-3767
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Kapanke, Bethany
AU  - Kapanke B
AUID- ORCID: 0000-0003-3340-9999
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Martinez, Danilo
AU  - Martinez D
AUID- ORCID: 0000-0002-0543-8517
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Xue, Shanyan
AU  - Xue S
AUID- ORCID: 0000-0001-8318-1967
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Suglia, Shakira F
AU  - Suglia SF
AUID- ORCID: 0000-0002-5634-9290
AD  - Department of Epidemiology, Rollins School of Public Health, Emory University, 
      Atlanta, Georgia, United States of America.
FAU - Bastain, Theresa M
AU  - Bastain TM
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
FAU - Marsit, Carmen
AU  - Marsit C
AD  - Gangarosa Department of Environmental Health, Rollins School of Public Health, 
      Emory University, Atlanta, Georgia, United States of America.
FAU - Breton, Carrie V
AU  - Breton CV
AUID- ORCID: 0000-0002-6082-4066
AD  - Department of Preventive Medicine, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, United States of America.
LA  - eng
GR  - P30 ES007048/ES/NIEHS NIH HHS/United States
GR  - P50 MD015705/MD/NIMHD NIH HHS/United States
GR  - R00 ES030400/ES/NIEHS NIH HHS/United States
GR  - R01 MD011698/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210512
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Cohort Studies
MH  - Exosomes/metabolism
MH  - Extracellular Vesicles/*genetics/metabolism
MH  - Female
MH  - Gene Expression/genetics
MH  - Gestational Age
MH  - Humans
MH  - Los Angeles
MH  - MicroRNAs/blood/*genetics
MH  - Middle Aged
MH  - Placenta/metabolism
MH  - Pregnancy/*genetics
MH  - Transcriptome/genetics
PMC - PMC8115775
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/05/13 06:00
MHDA- 2021/10/29 06:00
PMCR- 2021/05/12
CRDT- 2021/05/12 17:19
PHST- 2020/12/14 00:00 [received]
PHST- 2021/04/22 00:00 [accepted]
PHST- 2021/05/12 17:19 [entrez]
PHST- 2021/05/13 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
PHST- 2021/05/12 00:00 [pmc-release]
AID - PONE-D-20-39223 [pii]
AID - 10.1371/journal.pone.0251259 [doi]
PST - epublish
SO  - PLoS One. 2021 May 12;16(5):e0251259. doi: 10.1371/journal.pone.0251259. 
      eCollection 2021.