PMID- 33980176
OWN - NLM
STAT- MEDLINE
DCOM- 20210521
LR  - 20210521
IS  - 1471-2229 (Electronic)
IS  - 1471-2229 (Linking)
VI  - 21
IP  - 1
DP  - 2021 May 12
TI  - A universal karyotypic system for hexaploid and diploid Avena species brings oat 
      cytogenetics into the genomics era.
PG  - 213
LID - 10.1186/s12870-021-02999-3 [doi]
LID - 213
AB  - BACKGROUND: The identification of chromosomes among Avena species have been 
      studied by C-banding and in situ hybridization. However, the complicated results 
      from several cytogenetic nomenclatures for identifying oat chromosomes are often 
      contradictory. A universal karyotyping nomenclature system for precise chromosome 
      identification and comparative evolutionary studies would be essential for genus 
      Avena based on the recently released genome sequences of hexaploid and diploid 
      Avena species. RESULTS: Tandem repetitive sequences were predicted and physically 
      located on chromosomal regions of the released Avena sativa OT3098 genome 
      assembly v1. Eight new oligonucleotide (oligo) probes for sequential fluorescence 
      in situ hybridization (FISH) were designed and then applied for chromosome 
      karyotyping on mitotic metaphase spreads of A. brevis, A. nuda, A. wiestii, A. 
      ventricosa, A. fatua, and A. sativa species. We established a high-resolution 
      standard karyotype of A. sativa based on the distinct FISH signals of multiple 
      oligo probes. FISH painting with bulked oligos, based on wheat-barley collinear 
      regions, was used to validate the linkage group assignment for individual A. 
      sativa chromosomes. We integrated our new Oligo-FISH based karyotype system with 
      earlier karyotype nomenclatures through sequential C-banding and FISH methods, 
      then subsequently determined the precise breakage points of some chromosome 
      translocations in A. sativa. CONCLUSIONS: This new universal chromosome 
      identification system will be a powerful tool for describing the genetic 
      diversity, chromosomal rearrangements and evolutionary relationships among Avena 
      species by comparative cytogenetic and genomic approaches.
FAU - Jiang, Wenxi
AU  - Jiang W
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Jiang, Chengzhi
AU  - Jiang C
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Yuan, Weiguang
AU  - Yuan W
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Zhang, Meijun
AU  - Zhang M
AD  - College of Agronomy, Shanxi Agricultural University, 030801, Taigu, China.
FAU - Fang, Zijie
AU  - Fang Z
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Li, Yang
AU  - Li Y
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Li, Guangrong
AU  - Li G
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China.
FAU - Jia, Juqing
AU  - Jia J
AD  - College of Agronomy, Shanxi Agricultural University, 030801, Taigu, China. 
      jiajuqing@126.com.
FAU - Yang, Zujun
AU  - Yang Z
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, 611731, Chengdu, China. 
      yangzujun@uestc.edu.cn.
LA  - eng
GR  - 2016YFD0102000/National Key Research and Development Program of China/
GR  - 2016YFD0102000/National Key Research and Development Program of China/
GR  - 2016YFD0102000/National Key Research and Development Program of China/
GR  - 2016YFD0102000/National Key Research and Development Program of China/
GR  - 201903D221073/Shanxi Provincial Key Research and Development Project/
GR  - 201903D221073/Shanxi Provincial Key Research and Development Project/
GR  - 31971886/National Natural Science Foundation of China/
GR  - 31971886/National Natural Science Foundation of China/
GR  - 31971886/National Natural Science Foundation of China/
GR  - 31971886/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210512
PL  - England
TA  - BMC Plant Biol
JT  - BMC plant biology
JID - 100967807
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Avena/*classification/*genetics
MH  - Crops, Agricultural/classification/genetics
MH  - Cytogenetic Analysis
MH  - *DNA, Plant
MH  - *Diploidy
MH  - *Genome, Plant
MH  - Karyotyping/*classification
MH  - *Terminology as Topic
PMC - PMC8114715
OTO - NOTNLM
OT  - Avena
OT  - Chromosome identification
OT  - FISH
OT  - Oligo-probes
COIS- The authors declare that they have no competing interest.
EDAT- 2021/05/14 06:00
MHDA- 2021/05/22 06:00
PMCR- 2021/05/12
CRDT- 2021/05/13 05:31
PHST- 2021/02/11 00:00 [received]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/05/13 05:31 [entrez]
PHST- 2021/05/14 06:00 [pubmed]
PHST- 2021/05/22 06:00 [medline]
PHST- 2021/05/12 00:00 [pmc-release]
AID - 10.1186/s12870-021-02999-3 [pii]
AID - 2999 [pii]
AID - 10.1186/s12870-021-02999-3 [doi]
PST - epublish
SO  - BMC Plant Biol. 2021 May 12;21(1):213. doi: 10.1186/s12870-021-02999-3.