PMID- 33980325
OWN - NLM
STAT- MEDLINE
DCOM- 20211004
LR  - 20220823
IS  - 2769-6677 (Electronic)
IS  - 1559-6109 (Print)
IS  - 1559-6109 (Linking)
VI  - 60
IP  - 4
DP  - 2021 Jul 1
TI  - Assessment of Mitochondrial Function and Developmental Potential of Mouse Oocytes 
      after Mitoquinone Supplementation during Vitrification.
PG  - 388-395
LID - 10.30802/AALAS-JAALAS-20-000123 [doi]
AB  - Vitrification negatively affects the mitochondrial membrane potential (DeltaPsim) in 
      oocytes while also leading to increased reactive oxygen species (ROS), ATP 
      depletion and induction of apoptosis in oocytes. Mitoquinone (MitoQ) is an 
      antioxidant that protects mitochondrial membrane integrity from ROS. This study 
      examined the effect of adding MitoQ to vitrification medium on mitochondrial 
      function and embryo development in vitrified oocytes. Metaphase II (MII) stage 
      oocytes were collected from NMRI mouse ovaries and preincubated for 20 min in a 
      medium containing 0.02 muM of MitoQ. Next, oocytes were vitrified in medium 
      supplemented with 0.02muM of MitoQ (treatment group). The control group was 
      processed in the same way but without exposure to MitoQ. After warming, oocyte 
      survival rate, DeltaPsim, cytoplasmic ROS and glutathione (GSH) levels and gene 
      expression levels (Bcl2, BAX, and caspase3) were measured. In addition, the 
      vitrified oocytes were fertilized in-vitro to assess developmental competence. 
      The results showed that MitoQ improved survival and DeltaPsim in treated vitrified 
      oocytes. Treated oocytes showed lower ROS levels and higher GSH levels than did 
      the control group. Furthermore, mRNA expression of the Bax/ Bcl2 ratio and 
      caspase3 were significantly lower in treated oocytes. These findings indicate 
      that medium supplementation with 0.02 muM of MitoQ during vitrification can 
      improve oocyte survival and developmental competency in mouse oocytes.
FAU - Shirzeyli, Maryam H
AU  - Shirzeyli MH
AD  - Department of Anatomical Sciences, School of Medicine, Shahid Beheshti University 
      of Medical Sciences, Tehran, Iran.
FAU - Eini, Fatemeh
AU  - Eini F
AD  - Fertility and Infertility Research Center, Hormozgan University of Medical 
      Science, Bandar Abbas, Iran.
FAU - Shirzeyli, Farshad H
AU  - Shirzeyli FH
AD  - Department of Animal Science, Faculty of Agriculture and Natural Resources, 
      Science and Research branch, Islamic Azad University, Tehran, Iran.
FAU - Majd, Saeid A
AU  - Majd SA
AD  - Department of animal and marine biotechnology, National institute of genetic 
      Engineering and Biotechnology, Tehran, Iran.
FAU - Ghahremani, Mehrdad
AU  - Ghahremani M
AD  - Department of Obstetrics & Gynecology, Monash University, Clayton, VIC, 
      Australia.
FAU - Joupari, Morteza D
AU  - Joupari MD
AD  - Department of animal and marine biotechnology, National institute of genetic 
      Engineering and Biotechnology, Tehran, Iran.
FAU - Novin, Marefat G
AU  - Novin MG
AD  - Cellular and Molecular Biology Research Center, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran; mghaffarin@yahoo. com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210512
PL  - United States
TA  - J Am Assoc Lab Anim Sci
JT  - Journal of the American Association for Laboratory Animal Science : JAALAS
JID - 101269489
RN  - 0 (Organophosphorus Compounds)
RN  - 1339-63-5 (Ubiquinone)
RN  - 47BYS17IY0 (mitoquinone)
SB  - IM
MH  - Animals
MH  - Cryopreservation
MH  - Dietary Supplements
MH  - Mice
MH  - Mitochondria
MH  - *Oocytes/metabolism
MH  - Organophosphorus Compounds
MH  - Ubiquinone/analogs & derivatives
MH  - *Vitrification
PMC - PMC9390609
EDAT- 2021/05/14 06:00
MHDA- 2021/10/05 06:00
PMCR- 2022/01/01
CRDT- 2021/05/13 05:38
PHST- 2021/05/14 06:00 [pubmed]
PHST- 2021/10/05 06:00 [medline]
PHST- 2021/05/13 05:38 [entrez]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 2021000388 [pii]
AID - 10.30802/AALAS-JAALAS-20-000123 [doi]
PST - ppublish
SO  - J Am Assoc Lab Anim Sci. 2021 Jul 1;60(4):388-395. doi: 
      10.30802/AALAS-JAALAS-20-000123. Epub 2021 May 12.