PMID- 33981238
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210514
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 12
DP  - 2021
TI  - Bletilla striata Polysaccharide Promotes Diabetic Wound Healing Through 
      Inhibition of the NLRP3 Inflammasome.
PG  - 659215
LID - 10.3389/fphar.2021.659215 [doi]
LID - 659215
AB  - The aim of this study was to evaluate the therapeutic effects of Bletilla striata 
      polysaccharide (BSP) on wound healing in diabetes mellitus (DM) and to explore 
      the underlying mechanisms. DM mouse models were induced by high fat-diet feeding 
      combined with low-dose streptozocin injection. To establish diabetic foot ulcer 
      (DFU) models, DM mice were wounded on the dorsal surface. Subsequently, mice were 
      treated with vehicle or BSP for 12 days and wound healing was monitored. The 
      effects of BSP on the production of interleukin-1beta (IL-1beta), tumor necrosis 
      factor-alpha, macrophages infiltration, angiogenesis, the activation of 
      nucleotide-binding and oligomerization (NACHT) domain, leucine-rich repeat (LRR), 
      and pyrin domain (PYD)-containing protein 3 (NLRP3) inflammasome, and insulin 
      sensitivity in wound tissues were subsequently evaluated. Separated- and 
      cultured- bone marrow-derived macrophages (BMDMs) and cardiac microvascular 
      endothelial cells (CMECs) were isolated from mice and used to investigate the 
      effects of BSP on cell viability, reactive oxygen species (ROS) generation, NLRP3 
      inflammasome activation and insulin sensitivity in vitro following exposure to 
      high glucose (HG). BSP administration accelerated diabetic wound healing, 
      suppressed macrophage infiltration, promoted angiogenesis, suppressed NLRP3 
      inflammasome activation, decreased IL-1beta secretion, and improved insulin 
      sensitivity in wound tissues in DM mice. In vitro, co-treatment with BSP 
      protected against HG-induced ROS generation, NLRP3 inflammasome activation, and 
      IL-1beta secretion in BMDMs, and improved cell viability and decreased ROS levels in 
      CMECs. Moreover, in HG exposed BMDMs-CMECs cultures, BSP treatment suppressed 
      NLRP3 inflammasome activation and IL-1beta secretion in BMDMs, and improved cell 
      viability and insulin sensitivity in CMECs. Furthermore, treatment with IL-1beta 
      almost completely suppressed the beneficial effects of BSP on the NLRP3 
      inflammasome, IL-1beta secretion, and insulin sensitivity in HG-treated BMDMs-CMECs. 
      BSP promotes DFU healing through inhibition of the HG-activated NLRP3 
      inflammasome.
CI  - Copyright (c) 2021 Zhao, Wang, Yan, Zhou, Huang, Zhu, Ye, Zhang, Chen, Chen and 
      Zheng.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
AD  - College of Pharmacy, Hubei University of Medicine, Shiyan, China.
FAU - Wang, Qibin
AU  - Wang Q
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Yan, Shan
AU  - Yan S
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
AD  - College of Pharmacy, Hubei University of Medicine, Shiyan, China.
FAU - Zhou, Jun
AU  - Zhou J
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
AD  - College of Pharmacy, Hubei University of Medicine, Shiyan, China.
FAU - Huang, Liangyong
AU  - Huang L
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Zhu, Haitao
AU  - Zhu H
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Ye, Fang
AU  - Ye F
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Zhang, Yonghong
AU  - Zhang Y
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
FAU - Chen, Lin
AU  - Chen L
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Chen, Li
AU  - Chen L
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
FAU - Zheng, Tao
AU  - Zheng T
AD  - Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, China.
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210426
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC8110216
OTO - NOTNLM
OT  - Bletilla striata
OT  - NLRP3 inflammasome
OT  - diabetic foot ulcer
OT  - high glucose
OT  - polysaccharide
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/14 06:00
MHDA- 2021/05/14 06:01
PMCR- 2021/04/26
CRDT- 2021/05/13 06:24
PHST- 2021/01/27 00:00 [received]
PHST- 2021/04/12 00:00 [accepted]
PHST- 2021/05/13 06:24 [entrez]
PHST- 2021/05/14 06:00 [pubmed]
PHST- 2021/05/14 06:01 [medline]
PHST- 2021/04/26 00:00 [pmc-release]
AID - 659215 [pii]
AID - 10.3389/fphar.2021.659215 [doi]
PST - epublish
SO  - Front Pharmacol. 2021 Apr 26;12:659215. doi: 10.3389/fphar.2021.659215. 
      eCollection 2021.