PMID- 33983482
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20210622
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 78
IP  - 7
DP  - 2021 Jul
TI  - Distinct Pattern of Paracoccidioides lutzii, P. restrepiensis and P. americana 
      Antigens Recognized by IgE in Human Paracoccidioidomycosis.
PG  - 2608-2614
LID - 10.1007/s00284-021-02508-7 [doi]
AB  - Paracoccidioidomycosis (PCM) is caused by the fungi Paracoccidioides spp. The 
      main antigens recognized by IgE are known for P. brasiliensis species complex, 
      but not for P. lutzii. Current research investigated the major P. lutzii (LDR2) 
      antigens recognized by IgE, in comparison to P. restrepiensis and P. americana 
      (former P. brasiliensis species complex), besides IgG recognition. Cell-free 
      antigens (CFA) from P. lutzii (LDR2), P. restrepiensis (B339) and P. americana 
      (LDR3) were analyzed by ELISA and immunoblotting (IB) by detecting specific IgG 
      and IgE from sera from patients with PCM presumable by P. brasiliensis species 
      complex (n = 24). Additionally, somatic antigen (SA) was analyzed by IB. P. 
      lutzii (LDR2) antigens showed significantly lower reactivity than P. 
      restrepiensis (B339) and P. americana (LDR3) in ELISA for both IgE and IgG 
      (p < 0.05). The IgE-IB pattern was different between P. lutzii (LDR2) and the 
      other species, regarding components with ~30 kDa and ~70 kDa in CFA and a ~200 
      kDa in SA. P. lutzii (LDR2) present at least three antigens recognized by IgE 
      which mainly differ from P. restrepiensis (B339) and, to a lesser extent, from P. 
      americana (LDR3). Current research evidenced for the first time the major P. 
      lutzii (LDR2) antigens recognized by IgE.
FAU - Assolini, Joao P
AU  - Assolini JP
AUID- ORCID: 0000-0003-4190-8835
AD  - State University of Londrina, Londrina, PR, Brazil. jp_assolini22@hotmail.com.
AD  - Departamento de Ciencias Patologicas, CCB, Universidade Estadual de Londrina, 
      Campus Universitario, Londrina, PR, 86051-970, Brazil. jp_assolini22@hotmail.com.
FAU - Lenhard-Vidal, Adriane
AU  - Lenhard-Vidal A
AD  - State University of Londrina, Londrina, PR, Brazil.
FAU - Bredt, Carla S de O
AU  - Bredt CSO
AD  - State University of Western Parana, Cascavel, PR, Brazil.
FAU - Tano, Zuleica N
AU  - Tano ZN
AD  - State University of Londrina, Londrina, PR, Brazil.
FAU - Sano, Ayako
AU  - Sano A
AD  - Department of Animal Sciences, University of the Ryukyus, Okinawa, Japan.
FAU - Cezar-Dos-Santos, Fernando
AU  - Cezar-Dos-Santos F
AD  - State University of Londrina, Londrina, PR, Brazil.
FAU - Ono, Mario A
AU  - Ono MA
AD  - State University of Londrina, Londrina, PR, Brazil.
FAU - Itano, Eiko N
AU  - Itano EN
AD  - State University of Londrina, Londrina, PR, Brazil. itano@uel.br.
AD  - Departamento de Ciencias Patologicas, CCB, Universidade Estadual de Londrina, 
      Campus Universitario, Londrina, PR, 86051-970, Brazil. itano@uel.br.
LA  - eng
GR  - 976/2013/PPSUS/Fundacao Araucaria/
PT  - Journal Article
DEP - 20210513
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Antigens, Fungal)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - Paracoccidioides lutzii
SB  - IM
MH  - Antigens, Fungal
MH  - Humans
MH  - Immunoglobulin E
MH  - *Paracoccidioides
MH  - *Paracoccidioidomycosis
EDAT- 2021/05/14 06:00
MHDA- 2021/06/23 06:00
CRDT- 2021/05/13 12:37
PHST- 2020/08/10 00:00 [received]
PHST- 2021/04/25 00:00 [accepted]
PHST- 2021/05/14 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2021/05/13 12:37 [entrez]
AID - 10.1007/s00284-021-02508-7 [pii]
AID - 10.1007/s00284-021-02508-7 [doi]
PST - ppublish
SO  - Curr Microbiol. 2021 Jul;78(7):2608-2614. doi: 10.1007/s00284-021-02508-7. Epub 
      2021 May 13.