PMID- 33983982
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20240124
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 5
DP  - 2021
TI  - Gut microbiome differences among Mexican Americans with and without type 2 
      diabetes mellitus.
PG  - e0251245
LID - 10.1371/journal.pone.0251245 [doi]
LID - e0251245
AB  - PURPOSE: Type 2 diabetes mellitus (T2DM) is an urgent public health problem and 
      disproportionately affects Mexican Americans. The gut microbiome contributes to 
      the pathophysiology of diabetes; however, no studies have examined this 
      association in Mexican-Americans. The objective of this study was to compare gut 
      microbiome composition between Mexican-Americans with and without T2DM. METHODS: 
      This was a cross-sectional study of volunteers from San Antonio, TX. Subjects 
      were 18 years or older and self-identified as Mexican American. Subjects were 
      grouped by prior T2DM diagnosis. Eligible subjects attended a clinic visit to 
      provide demographic and medical information. Thereafter, subjects recorded their 
      dietary intake for three days and collected a stool sample on the fourth day. 
      Stool 16s rRNA sequences were classified into operational taxonomic units (OTUs) 
      via the mothur bayesian classifier and referenced to the Greengenes database. 
      Shannon diversity and bacterial taxa relative abundance were compared between 
      groups using the Wilcoxon rank sum test. Beta diversity was estimated using 
      Bray-Curtis indices and compared between groups using PERMANOVA. RESULTS: 
      Thirty-seven subjects were included, 14 (38%) with diabetes and 23 (62%) without 
      diabetes. Groups were well-matched by body mass index and comorbid conditions. 
      Shannon diversity was not significantly different between those with and without 
      T2DM (3.26 vs. 3.31; p = 0.341). Beta diversity was not significantly associated 
      with T2DM diagnosis (p = 0.201). The relative abundance of the most common 
      bacterial phyla and families did not significantly differ between groups; 
      however, 16 OTUs were significantly different between groups. CONCLUSIONS: 
      Although alpha diversity was not significantly different between diabetic and 
      non-diabetic Mexican Americans, the abundance of certain bacterial taxa were 
      significantly different between groups.
FAU - Kitten, Amanda K
AU  - Kitten AK
AD  - College of Pharmacy, The University of Texas at Austin, Austin, Texas, United 
      States of America.
AD  - Pharmacotherapy Education and Research Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
FAU - Ryan, Laurajo
AU  - Ryan L
AD  - College of Pharmacy, The University of Texas at Austin, Austin, Texas, United 
      States of America.
AD  - Pharmacotherapy Education and Research Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
FAU - Lee, Grace C
AU  - Lee GC
AD  - College of Pharmacy, The University of Texas at Austin, Austin, Texas, United 
      States of America.
AD  - Pharmacotherapy Education and Research Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
FAU - Flores, Bertha E
AU  - Flores BE
AD  - School of Nursing, University of Texas Health San Antonio, San Antonio, Texas, 
      United States of America.
AD  - Research to Advance Community Health Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
FAU - Reveles, Kelly R
AU  - Reveles KR
AUID- ORCID: 0000-0001-8880-4879
AD  - College of Pharmacy, The University of Texas at Austin, Austin, Texas, United 
      States of America.
AD  - Pharmacotherapy Education and Research Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
AD  - Research to Advance Community Health Center, University of Texas Health San 
      Antonio, San Antonio, Texas, United States of America.
LA  - eng
GR  - P30 AG044271/AG/NIA NIH HHS/United States
GR  - UL1 TR002645/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20210513
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aged
MH  - Bacteria/genetics
MH  - Body Mass Index
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/metabolism/*microbiology
MH  - Eating
MH  - Feces/microbiology
MH  - Female
MH  - Gastrointestinal Microbiome/*genetics/physiology
MH  - Humans
MH  - Male
MH  - Mexican Americans/genetics
MH  - Middle Aged
MH  - Obesity/metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Texas
PMC - PMC8118452
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/05/14 06:00
MHDA- 2021/10/29 06:00
PMCR- 2021/05/13
CRDT- 2021/05/13 17:24
PHST- 2021/01/25 00:00 [received]
PHST- 2021/04/22 00:00 [accepted]
PHST- 2021/05/13 17:24 [entrez]
PHST- 2021/05/14 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
PHST- 2021/05/13 00:00 [pmc-release]
AID - PONE-D-21-02685 [pii]
AID - 10.1371/journal.pone.0251245 [doi]
PST - epublish
SO  - PLoS One. 2021 May 13;16(5):e0251245. doi: 10.1371/journal.pone.0251245. 
      eCollection 2021.