PMID- 33987477
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210516
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 15
DP  - 2020
TI  - Calcium Phosphate Cement Causes Nucleus Pulposus Cell Degeneration through the 
      ERK Signaling Pathway.
PG  - 209-216
LID - 10.1515/biol-2020-0021 [doi]
AB  - While calcium phosphate cement (CPC) is recognized as one of the most likely 
      substitutes for the conventional Polymethylmethacrylate (PMMA), there are very 
      few studies about its intradiscal leakage consequences. Herein, the goal of our 
      study was to examine the effect of CPC particles on the ERK (extracellular 
      regulatory kinase) pathway in human nucleus pulposus cell (HNPC) degeneration. 
      Different concentrations of CPC particles (0.00 per thousand, 0.01 per thousand, 0.05 per thousand, 0.1 per thousand v/v) were 
      added to human nucleus pulposus cell cultures. After 10 days of treatment, HNPC 
      biological behaviors and degeneration degree were analyzed by CCK-8 assay, 
      crystal violet staining, flow cytometer and western blot. The effect of CPC on 
      the ERK pathway was also analyzed by western blot. After activating the ERK path 
      by overexpressing Ras, HNPCs' biological behaviors and degeneration degree were 
      analyzed again. We found that CPC particles had a negative effect on human 
      nucleus pulposus cells (HNPCs), which are mainly reflected in cell growth and the 
      cell cycle. After activation of the ERK signaling pathway, the negative effects 
      of CPC on cell growth and the cell cycle were significantly reduced and the 
      degeneration degree of HNPCs was reversed. CPC particles can probably block the 
      activation of the ERK pathway, thus causing the HNPCs' degeneration.
CI  - (c) 2020 Quan Zhou, et al. published by De Gruyter.
FAU - Zhou, Quan
AU  - Zhou Q
AD  - Department of Orthopaedic Surgery, The Affiliated Huai'an Hospital of Xuzhou 
      Medical University, Huai'an, Jiangsu 223002, China.
FAU - Wu, Cenhao
AU  - Wu C
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
FAU - Zha, Jiali
AU  - Zha J
AD  - Department of Orthopaedic Surgery, The Affiliated Huai'an Hospital of Xuzhou 
      Medical University, Huai'an, Jiangsu 223002, China.
FAU - Ge, Jun
AU  - Ge J
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
FAU - Yan, Qi
AU  - Yan Q
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
FAU - Wang, Yingjie
AU  - Wang Y
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
FAU - Song, Dawei
AU  - Song D
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
FAU - Zou, Jun
AU  - Zou J
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow 
      University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
LA  - eng
PT  - Journal Article
DEP - 20200506
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC8114789
OTO - NOTNLM
OT  - ERK signaling pathway
OT  - bone cement leakage
OT  - calcium phosphate cement (CPC)
OT  - cell growth and cell cycle
OT  - human nucleus pulposus cells' degeneration
COIS- Conflict of interest: Authors state no conflict of interest.
EDAT- 2021/05/15 06:00
MHDA- 2021/05/15 06:01
PMCR- 2020/05/06
CRDT- 2021/05/14 07:03
PHST- 2019/09/11 00:00 [received]
PHST- 2020/02/10 00:00 [accepted]
PHST- 2021/05/14 07:03 [entrez]
PHST- 2021/05/15 06:00 [pubmed]
PHST- 2021/05/15 06:01 [medline]
PHST- 2020/05/06 00:00 [pmc-release]
AID - biol-2020-0021 [pii]
AID - 10.1515/biol-2020-0021 [doi]
PST - epublish
SO  - Open Life Sci. 2020 May 6;15:209-216. doi: 10.1515/biol-2020-0021. eCollection 
      2020.