PMID- 33990642 OWN - NLM STAT- MEDLINE DCOM- 20211029 LR - 20231111 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 11 IP - 1 DP - 2021 May 14 TI - Aggrecan: a new biomarker for acute type A aortic dissection. PG - 10371 LID - 10.1038/s41598-021-89653-y [doi] LID - 10371 AB - Acute type A aortic dissection (ATAAD) constitutes a life-threatening aortic pathology with significant morbidity and mortality. Without surgical intervention the usual mortality rate averages between 1 and 2% per hour. Thus, an early diagnosis of ATAAD is of pivotal importance to direct the affected patients to the appropriate treatment. Preceding tests to find an appropriate biomarker showed among others an increased aggrecan (ACAN) mRNA expression in aortic tissue of ATAAD patients. As a consequence, we investigated whether ACAN is a potential biomarker for diagnosing ATAAD. Mean ACAN protein concentration showed a significantly higher plasma concentration in ATAAD patients (38.59 ng/mL, n = 33) compared to plasma of patients with thoracic aortic aneurysms (4.45 ng/mL, n = 13), patients with myocardial infarction (11.77 ng/mL, n = 18) and healthy volunteers (8.05 ng/mL, n = 12). Cardiac enzymes like creatine kinase MB and cardiac troponin T showed no correlation with ACAN levels in ATAAD patients. Receiver-operator characteristics (ROC) curve analysis for ATAAD patients versus control subjects an optimum discrimination limit of ACAN plasma levels at 14.3 ng/mL with a corresponding sensitivity of 97% and specificity of 81%. According to our findings ACAN is a reliable potential biomarker in plasma samples to detect ATAAD with high sensitivity and specificity. FAU - Konig, Karl C AU - Konig KC AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. koenigc@dhm.mhn.de. FAU - Lahm, Harald AU - Lahm H AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. lahm@dhm.mhn.de. FAU - Dressen, Martina AU - Dressen M AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. FAU - Doppler, Stefanie A AU - Doppler SA AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. FAU - Eichhorn, Stefan AU - Eichhorn S AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. FAU - Beck, Nicole AU - Beck N AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. FAU - Kraehschuetz, Kathrin AU - Kraehschuetz K AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. FAU - Doll, Sophia AU - Doll S AD - Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. FAU - Holdenrieder, Stefan AU - Holdenrieder S AD - Institute of Laboratory Medicine, German Heart Center Munich, Munich, Germany. FAU - Kastrati, Adnan AU - Kastrati A AD - Department of Cardiology, German Heart Center Munich, Munich, Germany. FAU - Lange, Rudiger AU - Lange R AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. AD - DZHK (German Center for Cardiovascular Research) - Partner Site Munich Heart Alliance, Munich, Germany. FAU - Krane, Markus AU - Krane M AD - Division of Experimental Surgery, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636, Munich, Germany. AD - DZHK (German Center for Cardiovascular Research) - Partner Site Munich Heart Alliance, Munich, Germany. LA - eng PT - Journal Article PT - Observational Study PT - Research Support, Non-U.S. Gov't DEP - 20210514 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (ACAN protein, human) RN - 0 (Aggrecans) RN - 0 (Biomarkers) RN - 0 (Troponin T) RN - EC 2.7.3.2 (Creatine Kinase, MB Form) SB - IM MH - Acute Disease MH - Aged MH - Aggrecans/*blood MH - Aortic Dissection/blood/*diagnosis/etiology MH - Aortic Aneurysm, Thoracic/blood/*diagnosis MH - Biomarkers/blood MH - Creatine Kinase, MB Form/blood MH - Diagnosis, Differential MH - Female MH - Healthy Volunteers MH - Humans MH - Male MH - Middle Aged MH - Myocardial Infarction/blood/*diagnosis MH - ROC Curve MH - Retrospective Studies MH - Troponin T/blood PMC - PMC8121825 COIS- The German Heart Center Munich is holder of a patent (EP-No 20 151 237.3) for: Diagnosis of an aortic dissection by detecting a specific biomarker in a blood sample. K.C.K., H. L., M.D., R.L. and M.K. are joint partners of the patent. The other authors aren t joint partners of the above mentioned patent. The authors without competing interests are in particular Nicole Beck, Stefanie Doppler, Sophia Doll, Kathrin Krahschutz, Stefan Eichhorn, Stefan Holdenrieder and Adnan Kastrati. EDAT- 2021/05/16 06:00 MHDA- 2021/10/30 06:00 PMCR- 2021/05/14 CRDT- 2021/05/15 05:58 PHST- 2021/01/03 00:00 [received] PHST- 2021/04/19 00:00 [accepted] PHST- 2021/05/15 05:58 [entrez] PHST- 2021/05/16 06:00 [pubmed] PHST- 2021/10/30 06:00 [medline] PHST- 2021/05/14 00:00 [pmc-release] AID - 10.1038/s41598-021-89653-y [pii] AID - 89653 [pii] AID - 10.1038/s41598-021-89653-y [doi] PST - epublish SO - Sci Rep. 2021 May 14;11(1):10371. doi: 10.1038/s41598-021-89653-y.