PMID- 33990644 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20210928 LR - 20230202 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 11 IP - 1 DP - 2021 May 14 TI - Micro-mechanical performance evaluation of expansive soil biotreated with indigenous bacteria using MICP method. PG - 10324 LID - 10.1038/s41598-021-89687-2 [doi] LID - 10324 AB - This study explored the effect of indigenous bacteria present in the soil to stabilized swelling behavior and improving the mechanical property of expansive soil. The objective of the research is to investigate the effectiveness of the biostimulation microbial induced calcite precipitation (MICP) for controlling the swelling-shrinkage behavior and improving shear strength of expansive soil. An attempt was made to develop an effective procedure to culture the indigenous bacteria for treating clays with varying plasticity and improve their engineering behavior. The detailed procedure has been investigated to effectively apply the MICP technique in clay soil, considering its low permeable nature. The applicability of biostimulation to clayey soils in minimizing their swelling potential and improving the strength is assessed. Both macroscale and microscale studies were conducted on untreated and biostimulated soils to observe changes in plasticity, strength, swelling, mineralogical, chemical characteristics. The present method has shown an effective alternative to improve the road pavement subgrade without affecting the eco-system of natural soil. The method investigated the effective way of providing the enrichment and cementation solution in clayey soil, which is the major concern in current literature. The study confirms that the calcite content has been increased with biostimulated MICP treatment up to 205% in the treated specimens and which future increased the unconfined compressive strength and split tensile strength. A reduction in the swelling pressure and swell strain is also observed. The results show that a cost-effect and eco-friendly method can be deployed for stabilizing the road pavement subgrades. The statistical assessment using multivariate analysis and hierarchical clustering dendrogram has been carried out to investigate the effect of the MICP treatment protocol on different soil and engineering parameters. FAU - Tiwari, Nitin AU - Tiwari N AUID- ORCID: 0000-0002-4507-7832 AD - Department of Civil Engineering, Indian Institute of Technology Indore, Indore, India. nitintiwari077@gmail.com. FAU - Satyam, Neelima AU - Satyam N AUID- ORCID: 0000-0002-5434-0671 AD - Department of Civil Engineering, Indian Institute of Technology Indore, Indore, India. FAU - Sharma, Meghna AU - Sharma M AUID- ORCID: 0000-0002-0351-1637 AD - Department of Civil Engineering, Indian Institute of Technology Indore, Indore, India. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210514 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 SB - IM PMC - PMC8121830 COIS- The authors declare no competing interests. EDAT- 2021/05/16 06:00 MHDA- 2021/05/16 06:01 PMCR- 2021/05/14 CRDT- 2021/05/15 05:59 PHST- 2020/12/19 00:00 [received] PHST- 2021/04/30 00:00 [accepted] PHST- 2021/05/15 05:59 [entrez] PHST- 2021/05/16 06:00 [pubmed] PHST- 2021/05/16 06:01 [medline] PHST- 2021/05/14 00:00 [pmc-release] AID - 10.1038/s41598-021-89687-2 [pii] AID - 89687 [pii] AID - 10.1038/s41598-021-89687-2 [doi] PST - epublish SO - Sci Rep. 2021 May 14;11(1):10324. doi: 10.1038/s41598-021-89687-2.