PMID- 33990899
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220318
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Print)
IS  - 0268-8921 (Linking)
VI  - 37
IP  - 2
DP  - 2022 Mar
TI  - MMP-3 plays a major role in calcium pantothenate-promoted wound healing after 
      fractional ablative laser treatment.
PG  - 887-894
LID - 10.1007/s10103-021-03328-8 [doi]
AB  - Ablative fractional laser treatment leads to a loss of matrix metalloproteinase-3 
      (MMP-3) expression; therefore, in the present in vitro study, we addressed the 
      role of MMP-3 and its regulation by calcium pantothenate in wound healing 
      processes at the molecular level. Utilizing confocal laser microscopy, we 
      investigated MMP-3 protein expression in fractional ablative CO(2) 
      laser-irradiated skin models. In addition, we established full-thickness 3D skin 
      models using fibroblasts and keratinocytes with a MMP-3 knockdown that were 
      irradiated with a fractional ablative Er:YAG laser to set superficial injuries 
      with standardized dimensions and minimal thermal damage to the surrounding 
      tissue. We revealed an upregulation of MMP-3 protein expression in 
      laser-irradiated skin models receiving aftercare treatment with calcium 
      pantothenate. Skin models with MMP-3 knockdown exhibited a slower wound closure 
      after laser treatment compared to controls. Gene expression profiling detected an 
      MMP-3 knockdown-dependent upregulation of cytokines and chemokines (e.g. IL-36B, 
      CXCL17, IL-37, CXCL5), antimicrobial peptides (e.g., S100A7, S100A12), epidermal 
      crosslinking enzymes (TGM5), and differentiation markers (e.g., LOR, KRT1, FLG2). 
      We also detected a downregulation of cathepsin V and MMP-10, both of which play a 
      prominent role in wound healing processes. After fractional ablative laser 
      injury, an aftercare treatment with calcium pantothenate accelerated wound 
      closure in MMP-3 expressing models faster than in MMP-3 knockdown models. Our 
      data substantiate a major role of MMP-3 in wound healing processes after ablative 
      laser treatments. For the first time, we could show that calcium pantothenate 
      exerts its wound healing-promoting effects at least partly via MMP-3.
CI  - (c) 2021. The Author(s).
FAU - Huth, Sebastian
AU  - Huth S
AUID- ORCID: 0000-0003-4701-3864
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany. shuth@ukaachen.de.
FAU - Huth, Laura
AU  - Huth L
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany.
FAU - Marquardt, Yvonne
AU  - Marquardt Y
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany.
FAU - Cheremkhina, Maria
AU  - Cheremkhina M
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany.
FAU - Heise, Ruth
AU  - Heise R
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany.
FAU - Baron, Jens Malte
AU  - Baron JM
AD  - Department of Dermatology and Allergology, Medical Faculty RWTH Aachen 
      University, Pauwelsstrasse 30, 52074, Aachen, Germany.
AD  - Interdisciplinary Center for Laser Medicine, Medical Faculty RWTH Aachen 
      University, Aachen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20210514
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 19F5HK2737 (Pantothenic Acid)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - *Lasers, Gas/therapeutic use
MH  - Matrix Metalloproteinase 3/metabolism
MH  - *Pantothenic Acid/metabolism
MH  - Skin/metabolism
MH  - Wound Healing
PMC - PMC8918166
OTO - NOTNLM
OT  - 3D skin model
OT  - Calcium pantothenate
OT  - Dexpanthenol
OT  - MMP-3
OT  - Wound healing
COIS- The authors declare no competing interests.
EDAT- 2021/05/16 06:00
MHDA- 2022/03/16 06:00
PMCR- 2021/05/14
CRDT- 2021/05/15 06:14
PHST- 2020/10/12 00:00 [received]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/05/16 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2021/05/15 06:14 [entrez]
PHST- 2021/05/14 00:00 [pmc-release]
AID - 10.1007/s10103-021-03328-8 [pii]
AID - 3328 [pii]
AID - 10.1007/s10103-021-03328-8 [doi]
PST - ppublish
SO  - Lasers Med Sci. 2022 Mar;37(2):887-894. doi: 10.1007/s10103-021-03328-8. Epub 
      2021 May 14.