PMID- 33991835
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 229
DP  - 2021 Jun
TI  - Functions of the GABAergic system on serum LH concentrations in pre-pubertal 
      Nellore heifers.
PG  - 106764
LID - S0378-4320(21)00079-8 [pii]
LID - 10.1016/j.anireprosci.2021.106764 [doi]
AB  - This study was conducted to evaluate the luteinizing hormone (LH) secretion 
      pattern after gamma-aminobutyric acid (GABA(A)) antagonist to determine the 
      effects of the GABAergic system on LH secretion during reproductive maturation in 
      pre-pubertal Nellore heifers. Nellore heifers (n = 10) were administered a 
      picrotoxin injection of 0.18 mg/kg, i.v. Blood samples were collected every 
      15 min for 3 h at different developmental stages (8, 10, 14 and 17 mo of age). 
      Plasma concentrations of LH were quantified using an RIA (sensitivity of 
      0.04 ng/mL and CV of 15 %). There was an interaction between treatment and age (P 
      = 0.034). Picrotoxin-treated heifers had lesser (P </=  0.05) LH mean 
      concentrations during a 3 h period at 10 and 17 mo of age compared to control 
      heifers (P </=  0.05). Comparing the period before and after Picrotoxin injection 
      in the same animals, there was a 33 % decrease in LH concentration at 8 mo of age 
      (P = 0.0165). These results indicate the GABAergic system has a stimulatory 
      function in inducing LH secretion in pre-pubertal Nellore heifers. These findings 
      corroborate previous results that GABA increases GnRH/LH secretion in other 
      species during the pre-pubertal period.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Cardoso, Daniel
AU  - Cardoso D
AD  - Biological Institute, Sao Paulo Agency for Agribusiness Technology, Secretary of 
      Agriculture and Food Supply, Sao Paulo, Brazil.
FAU - Cardoso, Rodolfo C
AU  - Cardoso RC
AD  - Department of Animal Science, Texas A&M University, USA.
FAU - de Paula Nogueira, Guilherme
AU  - de Paula Nogueira G
AD  - Department of Animal Production and Health, Sao Paulo State University, 
      Aracatuba, Brazil. Electronic address: guilherme.nogueira@unesp.br.
LA  - eng
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20210512
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (GABA Antagonists)
RN  - 0 (Receptors, GABA)
RN  - 124-87-8 (Picrotoxin)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Cattle/blood/*physiology
MH  - Female
MH  - GABA Antagonists/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Luteinizing Hormone/*blood
MH  - Picrotoxin/*pharmacology
MH  - Receptors, GABA/genetics/*metabolism
MH  - Sexual Maturation/*physiology
OTO - NOTNLM
OT  - Hypothalamus
OT  - Neuroendocrine
OT  - Picrotoxin
OT  - Puberty
OT  - Sexual maturation
EDAT- 2021/05/16 06:00
MHDA- 2021/11/25 06:00
CRDT- 2021/05/15 20:19
PHST- 2020/04/23 00:00 [received]
PHST- 2021/04/28 00:00 [revised]
PHST- 2021/05/03 00:00 [accepted]
PHST- 2021/05/16 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2021/05/15 20:19 [entrez]
AID - S0378-4320(21)00079-8 [pii]
AID - 10.1016/j.anireprosci.2021.106764 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2021 Jun;229:106764. doi: 10.1016/j.anireprosci.2021.106764. 
      Epub 2021 May 12.