PMID- 33996996
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20220423
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2021
DP  - 2021
TI  - Antiprostate Cancer Activity of Ineupatolide Isolated from Carpesium cernuum L.
PG  - 5515961
LID - 10.1155/2021/5515961 [doi]
LID - 5515961
AB  - OBJECTIVE: The aim of the study was to investigate the antiprostate cancer 
      effects and mechanism of ineupatolide (T-21), a natural product isolated from the 
      Compositae plant Carpesium cernuum L., on PC-3 human prostate cancer cells. 
      METHODS: The effect of T-21 on the proliferation of PC-3 cells was detected by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell migration, and 
      invasion experiments; the morphology of cell apoptosis was observed by 
      Hoechst-propidium iodide staining; the effects of T-21 on PC-3 cell apoptosis and 
      the cell cycle were evaluated by flow cytometry; and the effect of T-21 on the 
      expression levels of phosphorylated protein kinase B (p-AKT), AKT, X-linked 
      inhibitor of apoptosis protein (xlAP), procaspase-3, and poly (ADP-ribose) 
      polymerase (PARP) in PC-3 cells was measured by western blotting. RESULTS: T-21 
      significantly inhibited the proliferation of cells, and its half-maximal 
      inhibitory concentrations at 12, 24, and 48 h were 38.46 +/- 1.01, 24.63 +/- 0.70, 
      and 7.36 +/- 0.58 muM, respectively. T-21 may promote cell apoptosis in a 
      concentration-dependent manner and block the cell cycle in the G2 and S phases. 
      In addition, T-21 significantly reduced the protein expression levels of p-AKT, 
      AKT, xlAP, procaspase-3, and PARP. CONCLUSION: T-21 exhibits antiproliferation 
      effects on PC-3 cells by promoting apoptosis and arresting the cell cycle in the 
      G2 and S phases. The possible mechanism underlying its potential therapeutic 
      effects against prostate cancer is related to the AKT/xlAP pathway.
CI  - Copyright (c) 2021 Yuan-she Huang et al.
FAU - Huang, Yuan-She
AU  - Huang YS
AD  - College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, 
      China.
AD  - Anshun College, Anshun Guizhou 561000, China.
FAU - Mao, Jing-Xin
AU  - Mao JX
AUID- ORCID: 0000-0002-2813-1702
AD  - College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, 
      China.
FAU - Zhang, Lai
AU  - Zhang L
AD  - Anshun College, Anshun Guizhou 561000, China.
FAU - Guo, Hong-Wei
AU  - Guo HW
AD  - An Shun City People's Hospital, Anshun 561000, China.
FAU - Yan, Chen
AU  - Yan C
AUID- ORCID: 0000-0001-9563-5604
AD  - An Shun City People's Hospital, Anshun 561000, China.
FAU - Chen, Min
AU  - Chen M
AUID- ORCID: 0000-0002-0140-4192
AD  - College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210429
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Phytochemicals)
RN  - 0 (X-Linked Inhibitor of Apoptosis Protein)
RN  - 0 (XIAP protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/drug effects
MH  - Asteraceae/*chemistry
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - Male
MH  - PC-3 Cells
MH  - Phytochemicals/*pharmacology
MH  - Prostatic Neoplasms/*metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction/drug effects
MH  - X-Linked Inhibitor of Apoptosis Protein/metabolism
PMC - PMC8105106
COIS- All authors declare that there are no commercial or associative interests that 
      represent a conflict of interest in connection with the work submitted.
EDAT- 2021/05/18 06:00
MHDA- 2021/06/23 06:00
PMCR- 2021/04/29
CRDT- 2021/05/17 06:17
PHST- 2021/02/16 00:00 [received]
PHST- 2021/03/27 00:00 [revised]
PHST- 2021/04/09 00:00 [accepted]
PHST- 2021/05/17 06:17 [entrez]
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2021/04/29 00:00 [pmc-release]
AID - 10.1155/2021/5515961 [doi]
PST - epublish
SO  - Biomed Res Int. 2021 Apr 29;2021:5515961. doi: 10.1155/2021/5515961. eCollection 
      2021.