PMID- 33997180
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231111
IS  - 2352-3042 (Electronic)
IS  - 2352-4820 (Print)
IS  - 2352-3042 (Linking)
VI  - 8
IP  - 3
DP  - 2021 May
TI  - Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of 
      endogenous hSDF-1alpha expression participates in neural restoration in 
      hypoxic-ischemic brain damage rats.
PG  - 331-343
LID - 10.1016/j.gendis.2020.01.012 [doi]
AB  - The transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) can 
      promote hypoxic-ischemic brain damage (HIBD) nerve repair, but finding suitable 
      seed cells to optimize transplantation and improve treatment efficiency is an 
      urgent problem to be solved. In this study, we induced hUC-MSCs into 
      dedifferentiated hUC-MSCs (De-hUC-MSCs), and the morphology, stem cell surface 
      markers, proliferation and tri-directional differentiation ability of the 
      De-hUC-MSCs and hUC-MSCs were detected. A whole-gene chip was utilized for genome 
      cluster, gene ontology and KEGG pathway analyses of differentially expressed 
      genes. De-hUC-MSCs were transplanted into HIBD rats, and behavioral experiments 
      and immunofluorescence assays were used to assess the therapeutic effect. A 
      lentivirus vector for human stromal cell-derived factor-1 (hSDF-1alpha) was 
      constructed, and the role of hSDF-1alpha in the neuroprotective effect and mechanism 
      of De-hUC-MSCs was verified. De-hUC-MSCs displayed similar cell morphology, stem 
      cell surface marker expression, cell proliferation and even three-dimensional 
      differentiation ability as hUC-MSCs but exhibited greater treatment potential in 
      vivo. The reprogramming mechanism of hSDF-1alpha participated in the 
      dedifferentiation process. By successfully constructing a stable hSDF-1alpha cell 
      line, we found that De-hUC-MSCs might participate in nerve repair through the 
      hSDF-1alpha/CXCR4/PI3K/Akt pathway. De-hUC-MSCs reprogramming of endogenous hSDF-1alpha 
      expression may mediate the hSDF-1alpha/CXCR4/PI3K/Akt pathway involved in nerve 
      repair in HIBD rats.
CI  - (c) 2020 Chongqing Medical University. Production and hosting by Elsevier B.V.
FAU - Xiaoqin, Zhou
AU  - Xiaoqin Z
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Jia, Liu
AU  - Jia L
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Mengjie, Dai
AU  - Mengjie D
AD  - Department of Neonatology, Chongqing Health Center for Women and Children, 
      400021, PR China.
FAU - Jialu, Gu
AU  - Jialu G
AD  - Child Health Centre of Northwest Women and Children's Hospital, USA.
FAU - Yang, Bi
AU  - Yang B
AD  - Department of Pediatric Research Institute, Chongqing Key Laboratory of Child 
      Health and Nutrition, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Yuting, Wang
AU  - Yuting W
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Huajian, Hu
AU  - Huajian H
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Bo, Liu
AU  - Bo L
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Xiaojun, Zhang
AU  - Xiaojun Z
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Zhongyue, Li
AU  - Zhongyue L
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Jie, Chen
AU  - Jie C
AD  - Department of Pediatric Research Institute, Chongqing Key Laboratory of Child 
      Health and Nutrition, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Tingyu, Li
AU  - Tingyu L
AD  - Department of Pediatric Research Institute, Chongqing Key Laboratory of Child 
      Health and Nutrition, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
FAU - Xue, Zhan
AU  - Xue Z
AD  - Department of Gastroenterology, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), 401122, PR China.
AD  - International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Children's Hospital of Chongqing Medical University, 
      Chongqing, 401122, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200218
PL  - Netherlands
TA  - Genes Dis
JT  - Genes & diseases
JID - 101635967
PMC - PMC8093640
OTO - NOTNLM
OT  - Dedifferentiation
OT  - Human umbilical cord mesenchymal stem cells
OT  - Hypoxic-ischemic brain damage
OT  - Neurorestoration
OT  - Reprogramming
OT  - Stromal cell-derived factor-1
EDAT- 2020/02/18 00:00
MHDA- 2020/02/18 00:01
PMCR- 2020/02/18
CRDT- 2021/05/17 06:19
PHST- 2019/11/13 00:00 [received]
PHST- 2020/01/18 00:00 [accepted]
PHST- 2021/05/17 06:19 [entrez]
PHST- 2020/02/18 00:00 [pubmed]
PHST- 2020/02/18 00:01 [medline]
PHST- 2020/02/18 00:00 [pmc-release]
AID - S2352-3042(20)30020-9 [pii]
AID - 10.1016/j.gendis.2020.01.012 [doi]
PST - epublish
SO  - Genes Dis. 2020 Feb 18;8(3):331-343. doi: 10.1016/j.gendis.2020.01.012. 
      eCollection 2021 May.