PMID- 33997389 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210518 IS - 2405-8440 (Print) IS - 2405-8440 (Electronic) IS - 2405-8440 (Linking) VI - 7 IP - 4 DP - 2021 Apr TI - Postnatally induced metabolic and oxidative changes associated with maternal high-fat consumption were mildly affected by Quercetin-3-O-rutinoside treatment in rats. PG - e06847 LID - 10.1016/j.heliyon.2021.e06847 [doi] LID - e06847 AB - Oxidative stress is usually associated with prolonged intake of high-fat diet (HFD). However, little is known about the impact of maternal HFD on endogenous modulation of antioxidant-defence-enzyme-network, its link to adverse fetal growth and overall effects of Quercetin-3-o-rutinoside (QR) supplementation. Sprague-Dawley rats were initially assigned to normal diet (ND) or HFD for 8 weeks and mated. Post-conception, rats were further divided into four groups, of which two groups had diets supplemented with QR while others continued with their respective diets until delivery. Measurements include food and water consumption, physical parameters (body weight, body mass index (BMI) and fur appearance), oral glucose tolerance, lipid profiles, and placental/liver oxidative changes. We observed that water consumption was significantly increased in dams fed HFD without marked differences in food intake, body weight, BMI and glucose tolerance. Surprisingly, offspring of HFD-fed dams had reduced body weight marked by delayed fur appearance compared to the ND offspring. In dams, there were alterations in lipid profile. Lipid peroxidation was increased in the placenta and liver of gestational day (GD) 19 HFD-fed dams and their postnatal day (PND) 21 male offspring. There was evidence of HFD-induced nitrosative stress in dams and PND28 female offspring. Adaptive defence indicate decreased placenta and liver superoxide dismutase (SOD) levels as well as differential changes in total antioxidant capacity (TAC) and catalase (CAT) activity in HFD treated dams and their progenies. Overall, the results indicate that intrauterine metabolic alterations associated with maternal high-fat consumption may induce oxidative challenge in the offspring accompanied by mild developmental consequences, while QR supplementation has little or no beneficial effects. CI - (c) 2021 The Authors. FAU - Adeyemi, Toluwalope E AU - Adeyemi TE AD - Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa. FAU - Ajonijebu, Duyilemi C AU - Ajonijebu DC AD - Department of Physiology, School of Biomolecular and Chemical Sciences, Nelson Mandela University, Port Elizabeth, South Africa. FAU - Channa, Mahendra L AU - Channa ML AD - Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa. FAU - Nadar, Anand AU - Nadar A AD - Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa. LA - eng PT - Journal Article DEP - 20210428 PL - England TA - Heliyon JT - Heliyon JID - 101672560 PMC - PMC8102762 OTO - NOTNLM OT - Developmental alterations OT - High-fat diet OT - Intrauterine OT - Metabolic changes OT - Oxidative stress OT - Quercetin-3-O-rutinoside COIS- The authors declare no conflict of interest. EDAT- 2021/05/18 06:00 MHDA- 2021/05/18 06:01 PMCR- 2021/04/28 CRDT- 2021/05/17 06:22 PHST- 2020/09/29 00:00 [received] PHST- 2021/01/05 00:00 [revised] PHST- 2021/04/14 00:00 [accepted] PHST- 2021/05/17 06:22 [entrez] PHST- 2021/05/18 06:00 [pubmed] PHST- 2021/05/18 06:01 [medline] PHST- 2021/04/28 00:00 [pmc-release] AID - S2405-8440(21)00950-6 [pii] AID - e06847 [pii] AID - 10.1016/j.heliyon.2021.e06847 [doi] PST - epublish SO - Heliyon. 2021 Apr 28;7(4):e06847. doi: 10.1016/j.heliyon.2021.e06847. eCollection 2021 Apr.