PMID- 33998029
OWN - NLM
STAT- MEDLINE
DCOM- 20211021
LR  - 20220531
IS  - 1600-051X (Electronic)
IS  - 0303-6979 (Print)
IS  - 0303-6979 (Linking)
VI  - 48
IP  - 9
DP  - 2021 Sep
TI  - Comparing periodontitis biomarkers in saliva, oral rinse and gingival crevicular 
      fluid: A pilot study.
PG  - 1250-1259
LID - 10.1111/jcpe.13479 [doi]
AB  - AIM: To explore the feasibility of screening for periodontitis by measuring 
      biomarkers, namely total proteolytic activity (TPA), matrix metalloproteinase 
      (MMP)-8, chitinase, lysozyme or their combination, in saliva, oral rinse and 
      gingival crevicular fluid (GCF). MATERIAL AND METHODS: Subjects were recruited 
      among healthy/gingivitis individuals and untreated periodontitis patients in 
      Academic Centre for Dentistry Amsterdam (ACTA). All participants donated samples 
      of unstimulated whole saliva, oral rinse and GCF. The protein concentrations and 
      MMP-8 levels were determined by ELISA. Enzymatic activities were measured using 
      appropriate fluorogenic substrates. RESULTS: In oral rinse samples, periodontitis 
      patients (n = 19) exhibited significantly higher concentrations of MMP-8 and TPA 
      than controls (n = 20). MMP-8 in combination with chitinase explained 88% of the 
      variance and assigned a subject to control or periodontitis group, with best 
      accuracy (87.2%) in oral rinse. CONCLUSIONS: The combination of MMP-8 and 
      chitinase in the current oral rinse procedure has the potential to discriminate 
      periodontitis from periodontal health/gingivitis.
CI  - (c) 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & 
      Sons Ltd.
FAU - Katsiki, Panagiota
AU  - Katsiki P
AUID- ORCID: 0000-0003-3297-1218
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Nazmi, Kamran
AU  - Nazmi K
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Loos, Bruno G
AU  - Loos BG
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Laine, Marja L
AU  - Laine ML
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Schaap, Kim
AU  - Schaap K
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Hepdenizli, Esen
AU  - Hepdenizli E
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Bikker, Floris J
AU  - Bikker FJ
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Brand, Henk S
AU  - Brand HS
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Veerman, Enno C I
AU  - Veerman ECI
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Nicu, Elena A
AU  - Nicu EA
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Oprisdent, Sibiu, Romania.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210620
PL  - United States
TA  - J Clin Periodontol
JT  - Journal of clinical periodontology
JID - 0425123
RN  - 0 (Biomarkers)
RN  - EC 3.4.24.34 (Matrix Metalloproteinase 8)
SB  - IM
MH  - Biomarkers/analysis
MH  - Gingival Crevicular Fluid/chemistry
MH  - *Gingivitis/diagnosis
MH  - Humans
MH  - Matrix Metalloproteinase 8
MH  - *Periodontitis/diagnosis
MH  - Pilot Projects
MH  - Saliva/chemistry
PMC - PMC8453974
OTO - NOTNLM
OT  - MMP-8
OT  - biomarkers
OT  - chitinase
OT  - gingival crevicular fluid
OT  - modelling
OT  - oral rinse
OT  - periodontitis
OT  - principal component analysis (PCA)
OT  - protease
OT  - saliva
COIS- The authors declare that there are no conflicts of interest in this study.
EDAT- 2021/05/18 06:00
MHDA- 2022/06/01 06:00
PMCR- 2021/09/21
CRDT- 2021/05/17 06:37
PHST- 2021/03/24 00:00 [revised]
PHST- 2020/09/03 00:00 [received]
PHST- 2021/04/15 00:00 [accepted]
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2022/06/01 06:00 [medline]
PHST- 2021/05/17 06:37 [entrez]
PHST- 2021/09/21 00:00 [pmc-release]
AID - JCPE13479 [pii]
AID - 10.1111/jcpe.13479 [doi]
PST - ppublish
SO  - J Clin Periodontol. 2021 Sep;48(9):1250-1259. doi: 10.1111/jcpe.13479. Epub 2021 
      Jun 20.