PMID- 33998076
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 35
IP  - 6
DP  - 2021 Jun
TI  - The clinical significance of spondin 2 eccentric expression in peripheral blood 
      mononuclear cells in bronchial asthma.
PG  - e23764
LID - 10.1002/jcla.23764 [doi]
LID - e23764
AB  - BACKGROUND: Bronchial asthma (BA) was a heterogeneous disease characterized by 
      chronic airway inflammation. Spondin 2 (SPON2) was reported to be implicated in 
      the integrin pathway, protein metabolism, and drug-induced lupus erythematosus. 
      The purpose of this study was to evaluate the significance of SPON2 in BA 
      diagnosis and treatment. METHODS: Peripheral blood samples were obtained from 137 
      BA pediatric patients (61 mild-to-moderate BA and 76 severe BA) and 59 healthy 
      children. Subject's information, clinical indexes, pulmonary ventilation 
      functions were recorded in the two groups. Peripheral blood mononuclear cells 
      (PBMCs) were isolated from patients' samples. qRT-PCR and ELISA assays were 
      employed to examine the levels of SPON2 and inflammatory cytokines, respectively. 
      Pearson's correlation analysis confirmed the association between SPON2 and 
      inflammatory cytokines. Receiver operating characteristic (ROC) analysis was used 
      to evaluate the potentials of SPON2 in terms of BA detection and discriminating 
      against the severity of BA. RESULTS: Bioinformatics analysis showed that SPON2, 
      OLFM4, XIST, and TSIX were significantly upregulated, while KDM5D and RPS4Y1 were 
      reduced in BA. GO analysis verified that these six genes were mainly involved in 
      neutrophil degranulation, neutrophil activation involved in immune response, 
      neutrophil activation, and neutrophil-mediated immunity. After isolating PBMCs, 
      we found that SPON2 was remarkably increased in BA pediatric group compared with 
      healthy children, and the relative levels of SPON2 were related to the severity 
      of BA. The receiver operating characteristic (ROC) analysis revealed the high 
      potentials of SPON2 in BA diagnosis (AUC was 0.8080) and severity distinctions 
      (AUCs were 0.7341 and 0.8541, respectively). Also, we found that there were 
      significant differences in fractional exhaled nitric oxide (FeNO), forced 
      expiratory volume in 1 s (FEV1)%, FEV1/ forced vital capacity (FVC)%, 
      immunoglobulin E (IgE), serum eosinophils, and serum neutrophils between 
      mild-to-moderate BA group and severe BA group. Finally, SPON2 was negatively 
      correlated with IL-12 while positively associated with IL-4, IL-13, and IL-17A. 
      CONCLUSIONS: SPON2 was a viable biomarker for diagnosing and degree of severity 
      in BA, providing more insight into exploring BA and treatment's pathogenesis.
CI  - (c) 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Department of Pediatric, Shengzhou People's Hospital (the First Affiliated 
      Hospital of Zhejiang University Shengzhou Branch), Shengzhou, China.
FAU - Xiang, Cai-Xia
AU  - Xiang CX
AD  - Department of Pediatric, Shengzhou People's Hospital (the First Affiliated 
      Hospital of Zhejiang University Shengzhou Branch), Shengzhou, China.
FAU - Wei, Jin-Feng
AU  - Wei JF
AUID- ORCID: 0000-0002-1795-2046
AD  - Department of Respiratory, Hangzhou Children's Hospital, Hangzhou, China.
LA  - eng
GR  - 2021KY932/Medical and Healthy Science and Technology Project of Zhejiang 
      Province/
PT  - Journal Article
DEP - 20210516
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Interleukins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (SPON2 protein, human)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Area Under Curve
MH  - Asthma/*blood/diagnosis/etiology/genetics
MH  - Case-Control Studies
MH  - Child
MH  - Extracellular Matrix Proteins/*blood/genetics
MH  - Female
MH  - Forced Expiratory Volume
MH  - Gene Expression
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Interleukins/blood
MH  - Leukocytes, Mononuclear/pathology/*physiology
MH  - Male
MH  - Neoplasm Proteins/*blood/genetics
MH  - Patient Acuity
PMC - PMC8183911
OTO - NOTNLM
OT  - GO analysis
OT  - bronchial asthma
OT  - diagnosis
OT  - peripheral blood mononuclear cell
OT  - spondin 2
COIS- None.
EDAT- 2021/05/18 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/05/16
CRDT- 2021/05/17 06:40
PHST- 2021/03/04 00:00 [revised]
PHST- 2021/02/02 00:00 [received]
PHST- 2021/03/06 00:00 [accepted]
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/05/17 06:40 [entrez]
PHST- 2021/05/16 00:00 [pmc-release]
AID - JCLA23764 [pii]
AID - 10.1002/jcla.23764 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2021 Jun;35(6):e23764. doi: 10.1002/jcla.23764. Epub 2021 May 
      16.