PMID- 33999051
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 146
IP  - 10
DP  - 2021 May 21
TI  - Dual cascade isothermal amplification reaction based glucometer sensors for 
      point-of-care diagnostics of cancer-related microRNAs.
PG  - 3242-3250
LID - 10.1039/d1an00037c [doi]
AB  - The practical use of a point-of-care (POC) device is of particular interest in 
      performing liquid biopsies related to cancer. Herein, taking advantage of the 
      practical convenience of a commercially available personal glucose meter (PGM), 
      we report a convenient, low-cost and sensitive detection strategy for circulating 
      microRNA-155 (miRNA155) in human serum. First, miRNA155 in serum triggers the 
      catalyzed hairpin assembly (CHA) reaction, and then the CHA product is 
      specifically captured by the peptide nucleic acid (PNA) probes attached to the 
      surface of a 96-well plate, which in turn triggers the hybridization chain 
      reaction (HCR), resulting in the local enrichment of invertase. Next, 
      introduction of a substrate (sucrose) for the invertase results in the generation 
      of glucose, which can be detected by a PGM. In this sensor, neutrally charged PNA 
      (12 nt) is more likely to hybridize with the CHA products than with the 
      negatively charged DNA in kinetics, which improves the detection sensitivity and 
      specificity. Due to the synergistic isothermal amplification reaction between CHA 
      and HCR, the sensor is able to achieve a broad dynamic range (from 1 fM to 10 nM) 
      with a detection limit down to 0.36 fM (3 orders of magnitude lower than that 
      without HCR) and is capable of distinguishing single-base mismatched sequences. 
      Thus the convenient, sensitive, robust and low-cost PGM sensor makes on-site 
      nucleic acids detection possible, suggesting its great application prospect as a 
      promising POC device in cancer diagnostics.
FAU - Fu, Pan
AU  - Fu P
AD  - Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials 
      Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, P. R. 
      China.
FAU - Xu, Mengjia
AU  - Xu M
FAU - Xing, Shu
AU  - Xing S
FAU - Zhao, Yang
AU  - Zhao Y
FAU - Zhao, Chao
AU  - Zhao C
LA  - eng
PT  - Journal Article
DEP - 20210409
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - *Biosensing Techniques
MH  - Humans
MH  - Limit of Detection
MH  - *MicroRNAs/genetics
MH  - *Neoplasms/diagnosis/genetics
MH  - Nucleic Acid Amplification Techniques
MH  - Nucleic Acid Hybridization
MH  - Point-of-Care Testing
EDAT- 2021/05/18 06:00
MHDA- 2021/05/25 06:00
CRDT- 2021/05/17 12:35
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2021/05/17 12:35 [entrez]
AID - 10.1039/d1an00037c [doi]
PST - ppublish
SO  - Analyst. 2021 May 21;146(10):3242-3250. doi: 10.1039/d1an00037c. Epub 2021 Apr 9.