PMID- 34000522
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 170
DP  - 2021 Aug
TI  - Vitamin E and selenium supplementation synergistically alleviate the injury 
      induced by hydrogen peroxide in bovine granulosa cells.
PG  - 91-106
LID - S0093-691X(21)00147-3 [pii]
LID - 10.1016/j.theriogenology.2021.04.015 [doi]
AB  - Dairy cows are susceptible to reproductive disorders, which are thought to be 
      associated with oxidative stress. In the study, we investigated the effects of 
      vitamin E (VE) and selenium (Se) on the proliferation, apoptosis, and 
      steroidogenesis in bovine ovarian granulosa cells under hydrogen peroxide 
      (H(2)O(2)) - induced oxidative stress and elaborated the underlying mechanisms. 
      Our results showed that VE or Se could stimulate the granulosa cell 
      proliferation, possibly due to up-regulating the expression of CCND1 and 
      decreasing the P21 levels under oxidative stress. VE or Se treatment also 
      increased the secretion of estradiol (E2) and progesterone (P4), which could be 
      owing to improving the expression of genes associated with steroidogenesis (StAR, 
      HSD3beta1, and CYP19A1) expression. VE or Se treatment down-regulated the 
      apoptosis-related genes (BAX, CASP3) expression and decreased cell apoptosis. 
      Furthermore, VE or Se treatment inhibited reactive oxidative species (ROS) and 
      malondialdehyde (MDA) generation, increased total antioxidant capacity (T-AOC), 
      and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione 
      peroxidase (GSH-Px). Additionally, VE or Se treatment also alleviated the 
      endoplasmic reticulum stress, activated the nuclear factor erythroid 2-related 
      factor 2 (NRF2), and up-regulated the expression of its downstream genes, 
      including NQO1, HO-1, GCLM, GCLC. More importantly, compared with either VE or Se 
      treatment alone, their combined treatment showed a better protective effect 
      against oxidative damage. Overall, our results indicated that VE and Se 
      synergistically stimulated the granulosa cell proliferation and steroidogenesis, 
      decreased cell apoptosis, mitigated the endoplasmic reticulum stress by 
      activating the NRF2 signal pathway.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Wang, Meimei
AU  - Wang M
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Li, Yan
AU  - Li Y
AD  - College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 
      071000, China.
FAU - Molenaar, Adrian
AU  - Molenaar A
AD  - AgResearch Ltd., Grasslands Research Centre, Private Bag 11008, Palmerston North, 
      4442, New Zealand.
FAU - Li, Qiufeng
AU  - Li Q
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Cao, Yufeng
AU  - Cao Y
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Shen, Yizhao
AU  - Shen Y
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Chen, Panliang
AU  - Chen P
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Yan, Jinling
AU  - Yan J
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China.
FAU - Gao, Yanxia
AU  - Gao Y
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China. Electronic address: yanxia.gao@hebau.edu.cn.
FAU - Li, Jianguo
AU  - Li J
AD  - College of Animal Science and Technology, Hebei Agricultural University, Baoding, 
      Hebei, 071000, China. Electronic address: jgli@hebau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210505
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 1406-18-4 (Vitamin E)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis
MH  - Cattle
MH  - Dietary Supplements
MH  - Female
MH  - Granulosa Cells/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - NF-E2-Related Factor 2
MH  - Oxidative Stress
MH  - *Selenium/pharmacology
MH  - Vitamin E/pharmacology
OTO - NOTNLM
OT  - Bovine granulosa cells
OT  - Oxidative stress
OT  - Selenium
OT  - Vitamin E
COIS- Declaration of competing interest The authors declare that there is no conflict 
      of interest.
EDAT- 2021/05/18 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/05/17 20:24
PHST- 2021/01/22 00:00 [received]
PHST- 2021/04/20 00:00 [revised]
PHST- 2021/04/25 00:00 [accepted]
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/05/17 20:24 [entrez]
AID - S0093-691X(21)00147-3 [pii]
AID - 10.1016/j.theriogenology.2021.04.015 [doi]
PST - ppublish
SO  - Theriogenology. 2021 Aug;170:91-106. doi: 10.1016/j.theriogenology.2021.04.015. 
      Epub 2021 May 5.