PMID- 34000636
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210707
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 360
DP  - 2021 Oct 30
TI  - Competitive and noncompetitive fluorescence polarization immunoassays for the 
      detection of benzothiostrobin using FITC-labeled dendrimer-like peptides.
PG  - 130020
LID - S0308-8146(21)01026-8 [pii]
LID - 10.1016/j.foodchem.2021.130020 [doi]
AB  - Peptides obtained from phage display libraries are valuable reagents for 
      small-molecule immunoassays. However, their application in fluorescence 
      polarization immunoassays (FPIAs) is limited by phage particles. Here, monomer, 
      dendrimer-like dimer, tetramer peptidomimetic and anti-immunocomplex tracers were 
      designed and synthesized using lysine as special scaffolds and spacers to develop 
      competitive and noncompetitive FPIAs for benzothiostrobin. The affinity between 
      tracers and monoclonal antibodies or immunocomplexes increased with the tracer 
      valence. A higher signal-to-noise ratio and sensitivity could be generated in the 
      FPIAs based on tetramer tracers. The sensitivities of competitive (50% inhibitory 
      concentration) and noncompetitive (50% saturation concentration) FPIAs were 
      19.71 +/- 4.65 and 40.43 +/- 2.73 ng mL(-1), respectively. The spiked recoveries were 
      78.3%-105.2% with relative standard deviations (RSDs) of 0.7%-15.4% for the 
      competitive FPIA, while 78.7%-115.3% with RSDs of 0.7%-12.5% for the 
      noncompetitive FPIA. The amounts of benzothiostrobin in rice detected by the 
      FPIAs were consistent with those detected by high performance liquid 
      chromatography.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Chen, He
AU  - Chen H
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China.
FAU - You, Tianyang
AU  - You T
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China.
FAU - Zong, Lingfeng
AU  - Zong L
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China.
FAU - Mukhametova, Liliya I
AU  - Mukhametova LI
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, 
      Russia.
FAU - Zherdev, Dmitry O
AU  - Zherdev DO
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, 
      Russia.
FAU - Eremin, Sergei A
AU  - Eremin SA
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, 
      Russia.
FAU - Ding, Yuan
AU  - Ding Y
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China.
FAU - Wang, Minghua
AU  - Wang M
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China.
FAU - Hua, Xiude
AU  - Hua X
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China; State & Local Joint Engineering Research Center of Green Pesticide 
      Invention and Application, Nanjing 210095, China. Electronic address: 
      huaxiude@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210506
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Acrylates)
RN  - 0 (Benzothiazoles)
RN  - 0 (Dendrimers)
RN  - 0 (Peptides)
RN  - 0 (benzothiostrobin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Acrylates/*analysis
MH  - Benzothiazoles/*analysis
MH  - Dendrimers/*chemistry
MH  - Fluorescein-5-isothiocyanate/*chemistry
MH  - Fluorescence Polarization Immunoassay/*methods
MH  - Peptides/*chemistry
OTO - NOTNLM
OT  - Anti-immunocomplex
OT  - Benzothiostrobin
OT  - Dendrimer-like peptides
OT  - Fluorescence polarization immunoassay
OT  - Peptidomimetic
EDAT- 2021/05/18 06:00
MHDA- 2021/07/08 06:00
CRDT- 2021/05/17 20:28
PHST- 2020/08/25 00:00 [received]
PHST- 2021/04/22 00:00 [revised]
PHST- 2021/05/03 00:00 [accepted]
PHST- 2021/05/18 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2021/05/17 20:28 [entrez]
AID - S0308-8146(21)01026-8 [pii]
AID - 10.1016/j.foodchem.2021.130020 [doi]
PST - ppublish
SO  - Food Chem. 2021 Oct 30;360:130020. doi: 10.1016/j.foodchem.2021.130020. Epub 2021 
      May 6.