PMID- 34010709
OWN - NLM
STAT- MEDLINE
DCOM- 20220114
LR  - 20220114
IS  - 1878-5867 (Electronic)
IS  - 0039-128X (Linking)
VI  - 172
DP  - 2021 Aug
TI  - In vitro evaluation of ferutinin on proliferation and osteogenesis 
      differentiation in human unrestricted Somatic stem cells.
PG  - 108862
LID - S0039-128X(21)00074-X [pii]
LID - 10.1016/j.steroids.2021.108862 [doi]
AB  - Osteoporosis is a common disease in post-menopausal women. The increased risk of 
      breast cancer and malignancy with hormone replacement, hampers its wide-usage. 
      Phytoestrogens are known to have selective estrogen receptor modulator activity. 
      The present study aims to determine how ferutinin affects unrestricted human 
      Somatic Stem Cells (USSCs) osteogenic differentiation. The effect of ferutinin on 
      USSCs proliferation was assessed by MTT assay while osteogenesis was evaluated 
      using Alkaline Phosphatase Activity (ALP), calcium deposition and Alizarin Red 
      Staining. Quantitative real-time PCR was applied to examine the expression of 
      bone specific genes such as osteocalcin, Runx(2), and BMP-(2). Ferutinin 
      (5-15 microg/mL) could positively impact on the proliferation of cells in a 
      dose-dependent manner. Also, ALP enzyme activity and calcium deposition were 
      enhanced in the presence of ferutinin. Based on real-time PCR results, ferutinin 
      could increase the expression of bone marker genes. The pattern of ferutinin 
      effect on gene expression is similar to standard synthetic estrogen, 
      17-beta-estradiol. In the presence of the estrogen activity inhibitor (ICI), the 
      effect of ferutinin on ALP and gene level was diminished. In conclusion, 
      ferutinin may be considered as a potential candidate for the stem cell therapy in 
      osteoporosis.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Mahmoudi, Zahra
AU  - Mahmoudi Z
AD  - Department of Life Science Engineering, Faculty of New Sciences and Technologies, 
      University of Tehran, Tehran, Iran.
FAU - Saidi, Abbas
AU  - Saidi A
AD  - Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and 
      Biotechnology, Shahid Beheshti University, Tehran, Iran.
FAU - Iranshahi, Mehrdad
AU  - Iranshahi M
AD  - Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Dadgar, Neda
AU  - Dadgar N
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Azizsoltani, Arezou
AU  - Azizsoltani A
AD  - Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz 
      University of Medical Science, Tabriz, Iran.
FAU - Behzad, Sahar
AU  - Behzad S
AD  - Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz 
      University of Medical Sciences, Karaj, Iran; Department of Pharmacognosy, School 
      of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 
      Electronic address: s.behzad@sbmu.ac.ir.
FAU - Mahmoudi, Ladan
AU  - Mahmoudi L
AD  - Department of Food Science and Technology, Faculty of Agriculture, Tabriz 
      University, Tabriz, Iran.
FAU - Soleimani, Masoud
AU  - Soleimani M
AD  - Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Parsa Khankandi, Hamed
AU  - Parsa Khankandi H
AD  - Department of Pharmacognosy, Facultyl of Pharmacy, Ardabil University of Medical 
      Sciences, Ardabil, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210516
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (Benzoates)
RN  - 0 (Bridged Bicyclo Compounds)
RN  - 0 (Cycloheptanes)
RN  - 0 (Plant Extracts)
RN  - 0 (Sesquiterpenes)
RN  - 41743-44-6 (4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en)
SB  - IM
MH  - Adult Stem Cells/*cytology/drug effects/metabolism
MH  - Benzoates/*pharmacology
MH  - Bridged Bicyclo Compounds/pharmacology
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cycloheptanes/*pharmacology
MH  - Ferula/chemistry
MH  - Fetal Blood/*cytology/drug effects/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - *Osteogenesis
MH  - Plant Extracts/*pharmacology
MH  - Sesquiterpenes/*pharmacology
OTO - NOTNLM
OT  - Ferula species
OT  - Ferutinin
OT  - Human unrestricted Somatic stem cells
OT  - Osteogenesis
OT  - Osteoporosis
EDAT- 2021/05/20 06:00
MHDA- 2022/01/15 06:00
CRDT- 2021/05/19 20:11
PHST- 2020/12/19 00:00 [received]
PHST- 2021/04/01 00:00 [revised]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/05/20 06:00 [pubmed]
PHST- 2022/01/15 06:00 [medline]
PHST- 2021/05/19 20:11 [entrez]
AID - S0039-128X(21)00074-X [pii]
AID - 10.1016/j.steroids.2021.108862 [doi]
PST - ppublish
SO  - Steroids. 2021 Aug;172:108862. doi: 10.1016/j.steroids.2021.108862. Epub 2021 May 
      16.