PMID- 34015180
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1744-7917 (Electronic)
IS  - 1672-9609 (Linking)
VI  - 29
IP  - 2
DP  - 2022 Apr
TI  - Molting process revealed by the detailed expression profiles of RXR1/RXR2 and 
      mining the associated genes in a spider mite, Panonychus citri.
PG  - 430-442
LID - 10.1111/1744-7917.12931 [doi]
AB  - Spider mites have one ecdysone receptor (EcR) and multiple retinoid X receptors 
      (RXRs). However, the function of these RXRs in spider mite development is 
      unknown. Here, we screened the expression dynamics of two PcRXR isoforms at 4 h 
      intervals in the deutonymphal stage of Panonychus citri. The results showed that 
      PcEcR had an expression pattern similar to that of PcRXR2. For PcRXR1, its 
      expression remained at a certain high level, when there was a decrease of both 
      PcEcR and PcRXR2. In situ hybridization showed that PcRXR2 was detected in the 
      central nervous mass, while the ecdysteroid biosynthesis gene PcSpo was mainly 
      expressed at the edge of the central nervous mass. RNAi-based silencing of PcRXR1 
      or PcRXR2 showed the same phenotype as in mites with that of silencing PcEcR. 
      Furthermore, RNA-seq was used to mine the genes associated with the expression 
      dynamics of PcRXR1 or PcRXR2, which revealed that the heterodimer of EcR-RXR2 in 
      spider mites might be linked with the cell autophagy and tissue remodeling during 
      apolysis, and RXR1 might be linked with new epicuticle and exocuticle secretion 
      during ecdysis. Taken together, these results increase our understanding of the 
      regulation mechanism of ecdysteroid signal pathway in spider mite development.
CI  - (c) 2021 Institute of Zoology, Chinese Academy of Sciences.
FAU - Li, Gang
AU  - Li G
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection; International Joint Laboratory of China-Belgium on Sustainable Crop 
      Pest Control, Academy of Agricultural Sciences; Southwest University, Chongqing, 
      China.
AD  - Provincial Key Laboratory for Agricultural Pest Management of Mountainous 
      Regions, Institute of Entomology, Guizhou University, Guiyang, China.
FAU - Liu, Xun-Yan
AU  - Liu XY
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection; International Joint Laboratory of China-Belgium on Sustainable Crop 
      Pest Control, Academy of Agricultural Sciences; Southwest University, Chongqing, 
      China.
FAU - Smagghe, Guy
AU  - Smagghe G
AUID- ORCID: 0000-0001-8334-3313
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection; International Joint Laboratory of China-Belgium on Sustainable Crop 
      Pest Control, Academy of Agricultural Sciences; Southwest University, Chongqing, 
      China.
AD  - Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent 
      University, Ghent, Belgium.
FAU - Niu, Jin-Zhi
AU  - Niu JZ
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection; International Joint Laboratory of China-Belgium on Sustainable Crop 
      Pest Control, Academy of Agricultural Sciences; Southwest University, Chongqing, 
      China.
FAU - Wang, Jin-Jun
AU  - Wang JJ
AUID- ORCID: 0000-0002-8777-5268
AD  - Key Laboratory of Entomology and Pest Control Engineering, College of Plant 
      Protection; International Joint Laboratory of China-Belgium on Sustainable Crop 
      Pest Control, Academy of Agricultural Sciences; Southwest University, Chongqing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210909
PL  - Australia
TA  - Insect Sci
JT  - Insect science
JID - 101266965
RN  - 0 (Ecdysteroids)
SB  - IM
MH  - Animals
MH  - Ecdysteroids
MH  - *Mites
MH  - Molting/genetics
MH  - RNA Interference
MH  - *Tetranychidae/genetics
OTO - NOTNLM
OT  - RNA interference
OT  - RNA-seq
OT  - ecdysone receptor (EcR)
OT  - in situ hybridization
OT  - retinoid X receptors
EDAT- 2021/05/21 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/05/20 18:22
PHST- 2021/04/04 00:00 [revised]
PHST- 2020/12/07 00:00 [received]
PHST- 2021/04/07 00:00 [accepted]
PHST- 2021/05/21 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/05/20 18:22 [entrez]
AID - 10.1111/1744-7917.12931 [doi]
PST - ppublish
SO  - Insect Sci. 2022 Apr;29(2):430-442. doi: 10.1111/1744-7917.12931. Epub 2021 Sep 
      9.