PMID- 34015592
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 202
DP  - 2021 Aug 5
TI  - A validated UHPLC-MS/MS method for pharmacokinetic and brain distribution studies 
      of twenty constituents in rat after oral administration of Jia-Wei-Qi-Fu-Yin.
PG  - 114140
LID - S0731-7085(21)00251-X [pii]
LID - 10.1016/j.jpba.2021.114140 [doi]
AB  - A rapid ultra-high performance liquid chromatography coupled with triple 
      quadrupole tandem mass spectrometry (UHPLC-QqQ MS/MS) approach with high 
      sensitivity and selectivity was developed for the quantification of twenty 
      compounds, including 9 saponins, 8 flavonoids, 2 oligosaccharide esters and 1 
      phenolic acid, in rat plasma and brain, which was administrated intragastrically 
      with Jia-Wei-Qi-Fu-Yin (JWQFY), Mass spectrometric detection was operated under 
      multiple reaction monitoring (MRM) mode. All calibration curves possessed good 
      linearity with correlation coefficients ( r(2)) higher than 0.9916 in their 
      respective linear ranges. For intra- and inter-day precision, all the relative 
      standard deviations (RSDs) at different levels were less than 14.68 %. Based on 
      the UHPLC-QqQ MS/MS quantitative results, pharmacokinetic study and brain 
      distribution of multiple components in JWQFY was then successfully performed. As 
      a result, constituents with discrepancy structures showed diverse pharmacokinetic 
      and distribution characteristics. For instance, ferulic acid (phenolic acid), 3, 
      6'-disinapoyl sucrose and tenuifoliside A (oligosaccharide esters) showed short 
      T(max) (< 10 min), whereas the T(max) of ginsenosides Rb(1), Rb(2) and Rc 
      (ppd-type terpenoid saponins) were much longer (> 4 h). Besides, ferulic acid, 
      epimedin C, icariin, glycyrrhizin, ginsenoside Rb(1) and ginsenoside Rg(1) were 
      considered as the potential effective ingredients of JWQFY because of their 
      relatively high exposure to blood and brain. Our study would provide relevant 
      information for discovery of pharmacodynamic ingredients, as well as further 
      action mechanisms investigations of JWQFY.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - An, Hai-Ming
AU  - An HM
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Li, Meng-Ning
AU  - Li MN
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Yang, Hua
AU  - Yang H
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Pang, Han-Qing
AU  - Pang HQ
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Qu, Cheng
AU  - Qu C
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Xu, Yi
AU  - Xu Y
AD  - Beijing Zhongyan Tongrentang Pharmaceutical R&D co., Ltd, Beijing, 100079, China.
FAU - Liu, Run-Zhou
AU  - Liu RZ
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Peng, Chao
AU  - Peng C
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China.
FAU - Li, Ping
AU  - Li P
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China. Electronic address: liping2004@126.com.
FAU - Gao, Wen
AU  - Gao W
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, 
      China. Electronic address: gw_cpu@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210513
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (qi-fu-yin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Brain
MH  - Chromatography, High Pressure Liquid
MH  - *Drugs, Chinese Herbal
MH  - Rats
MH  - Reproducibility of Results
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Brain distribution
OT  - Jia-Wei-Qi-Fu-Yin
OT  - Multiple constituents
OT  - Pharmacokinetics
OT  - UHPLC-MS/MS
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/05/21 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/05/20 20:18
PHST- 2020/12/17 00:00 [received]
PHST- 2021/04/09 00:00 [revised]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/05/21 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/05/20 20:18 [entrez]
AID - S0731-7085(21)00251-X [pii]
AID - 10.1016/j.jpba.2021.114140 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2021 Aug 5;202:114140. doi: 10.1016/j.jpba.2021.114140. Epub 
      2021 May 13.