PMID- 34015634
OWN - NLM
STAT- MEDLINE
DCOM- 20210630
LR  - 20210630
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 220
DP  - 2021 Sep 1
TI  - In vitro co-culture model of human monocyte-derived dendritic cells and T cells 
      to evaluate the sensitization of dinitrochlorobenzene.
PG  - 112331
LID - S0147-6513(21)00442-5 [pii]
LID - 10.1016/j.ecoenv.2021.112331 [doi]
AB  - Exposure to sensitizer has been suggested to be hazardous to human health, 
      evaluation the sensitization of sensitizer is particularly important and urgently 
      needed. Dendritic cells (DCs) exert an irreplaceable function in immunity, and 
      the T cell receptor (TCR) repertoire is key to ensuring immune response to 
      foreign antigens. We hypothesized that a co-culture model of human 
      monocyte-derived dendritic cells (Mo-DCs) and T cells could be employed to 
      evaluate the sensitization of DNCB. An experimental model of DNCB-induced 
      sensitization in rat was employed to examine alterations of cluster of 
      differentiation CD103(+) DCs and T cells. A co-cultured model of Mo-DCs and T 
      cells was developed in vitro to assess the sensitization of DNCB through the 
      phenotypic and functional alterations of Mo-DCs, as well as the TCR repertoire. 
      We found that the CD103(+) DCs phenotype and T-helper (Th) cells polarization 
      altered in sensitization rats. In vitro, phenotypic alteration of Mo-DCs caused 
      by DNCB were consistent with in vivo results, antigen uptake capacity of Mo-DCs 
      diminished and capacity of Mo-DCs to prime T cell increased. Clones of the TCR 
      repertoire and the diversity of TCR repertoire were enhanced, changes were noted 
      in the usage of variable, joining, and variable-joining gene combinations. DNCB 
      exposure potentiated alterations and characteristics of Mo-DCs and the TCR 
      repertoire in a co-culture model. Such changes provided innovative ideas for 
      evaluating sensitization of DNCB.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Bao, Lei
AU  - Bao L
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Hebei Medical University, Shijiazhuang, Hebei 050017, China; Department 
      of Occupational Health and Environmental Health, School of Public Health, 
      Zhengzhou University, Zhengzhou, Henan 450001, China; Hebei Key Laboratory of 
      Environment and Human Health, Shijiazhuang 050017, China.
FAU - Hao, Changfu
AU  - Hao C
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Zhengzhou University, Zhengzhou, Henan 450001, China.
FAU - Wang, Juan
AU  - Wang J
AD  - Department of Staistics, Hebei General Hospital, Shijiazhuang, Hebei 050000, 
      China.
FAU - Guo, Feifei
AU  - Guo F
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
FAU - Geng, Zihan
AU  - Geng Z
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
FAU - Wang, Di
AU  - Wang D
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Zhengzhou University, Zhengzhou, Henan 450001, China.
FAU - Zhao, Youliang
AU  - Zhao Y
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Zhengzhou University, Zhengzhou, Henan 450001, China.
FAU - Li, Yiping
AU  - Li Y
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Zhengzhou University, Zhengzhou, Henan 450001, China.
FAU - Yao, Wu
AU  - Yao W
AD  - Department of Occupational Health and Environmental Health, School of Public 
      Health, Zhengzhou University, Zhengzhou, Henan 450001, China. Electronic address: 
      yaowu@zzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210518
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Irritants)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Coculture Techniques
MH  - Dendritic Cells/*drug effects
MH  - Dinitrochlorobenzene/*toxicity
MH  - Female
MH  - Humans
MH  - Irritants/*toxicity
MH  - Male
MH  - Monocytes/cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - T-Lymphocytes/*drug effects
MH  - Young Adult
OTO - NOTNLM
OT  - Dendritic cell
OT  - Dinitrochlorobenzene
OT  - Evaluation of sensitization
OT  - T cell receptor
OT  - TCR repertoire
EDAT- 2021/05/21 06:00
MHDA- 2021/07/01 06:00
CRDT- 2021/05/20 20:21
PHST- 2021/01/25 00:00 [received]
PHST- 2021/04/14 00:00 [revised]
PHST- 2021/05/08 00:00 [accepted]
PHST- 2021/05/21 06:00 [pubmed]
PHST- 2021/07/01 06:00 [medline]
PHST- 2021/05/20 20:21 [entrez]
AID - S0147-6513(21)00442-5 [pii]
AID - 10.1016/j.ecoenv.2021.112331 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2021 Sep 1;220:112331. doi: 10.1016/j.ecoenv.2021.112331. 
      Epub 2021 May 18.