PMID- 34017314
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210523
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 12
DP  - 2021
TI  - RNase J1 and J2 Are Host-Encoded Factors for Plasmid Replication.
PG  - 586886
LID - 10.3389/fmicb.2021.586886 [doi]
LID - 586886
AB  - Plasmids need to ensure their transmission to both daughter-cells when their host 
      divides, but should at the same time avoid overtaxing their hosts by directing 
      excessive host-resources toward production of plasmid factors. Naturally 
      occurring plasmids have therefore evolved regulatory mechanisms to restrict their 
      copy-number in response to the volume of the cytoplasm. In many plasmid families, 
      copy-number control is mediated by a small plasmid-specified RNA, which is 
      continuously produced and rapidly degraded, to ensure that its concentration is 
      proportional to the current plasmid copy-number. We show here that pSA564 from 
      the RepA_N-family is regulated by a small antisense RNA (RNA1), which, when 
      over-expressed in trans, blocks plasmid replication and cures the bacterial host. 
      The 5' untranslated region (5'UTR) of the plasmid replication initiation gene 
      (repA) potentially forms two mutually exclusive secondary structures, ON and OFF, 
      where the latter both sequesters the repA ribosome binding site and acts as a 
      rho-independent transcriptional terminator. Duplex formation between RNA1 and the 
      5'UTR shifts the equilibrium to favor the putative OFF-structure, enabling a 
      single small RNA to down-regulate repA expression at both transcriptional and 
      translational levels. We further examine which sequence elements on the antisense 
      RNA and on its 5'UTR target are needed for this regulation. Finally, we identify 
      the host-encoded exoribonucleases RNase J1 and J2 as the enzymes responsible for 
      rapidly degrading the replication-inhibiting section of RNA1. This region 
      accumulates and blocks RepA expression in the absence of either RNase J1 or J2, 
      which are therefore essential host factors for pSA564 replication in 
      Staphylococcus aureus.
CI  - Copyright (c) 2021 Guimaraes, Le Scornet, Khemici, Hausmann, Armitano, Prados, 
      Jousselin, Manzano, Linder and Redder.
FAU - Guimaraes, Vanessa Andrade
AU  - Guimaraes VA
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Le Scornet, Alexandre
AU  - Le Scornet A
AD  - Laboratoire de Microbiologie et Genetique Moleculaires, Centre de Biologie 
      Integrative, Paul Sabatier University, Toulouse, France.
FAU - Khemici, Vanessa
AU  - Khemici V
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Hausmann, Stephane
AU  - Hausmann S
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Armitano, Joshua
AU  - Armitano J
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Prados, Julien
AU  - Prados J
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Jousselin, Ambre
AU  - Jousselin A
AD  - Laboratoire de Microbiologie et Genetique Moleculaires, Centre de Biologie 
      Integrative, Paul Sabatier University, Toulouse, France.
FAU - Manzano, Caroline
AU  - Manzano C
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Linder, Patrick
AU  - Linder P
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
FAU - Redder, Peter
AU  - Redder P
AD  - Department of Microbiology and Molecular Medicine, Faculty of Medicine, 
      University of Geneva, Geneva, Switzerland.
AD  - Laboratoire de Microbiologie et Genetique Moleculaires, Centre de Biologie 
      Integrative, Paul Sabatier University, Toulouse, France.
LA  - eng
PT  - Journal Article
DEP - 20210504
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8129170
OTO - NOTNLM
OT  - RNase J
OT  - Staphylococcus aureus
OT  - antisense RNA
OT  - essential host factors
OT  - plasmid replication control
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/22 06:00
MHDA- 2021/05/22 06:01
PMCR- 2021/05/04
CRDT- 2021/05/21 06:31
PHST- 2020/07/24 00:00 [received]
PHST- 2021/04/06 00:00 [accepted]
PHST- 2021/05/21 06:31 [entrez]
PHST- 2021/05/22 06:00 [pubmed]
PHST- 2021/05/22 06:01 [medline]
PHST- 2021/05/04 00:00 [pmc-release]
AID - 10.3389/fmicb.2021.586886 [doi]
PST - epublish
SO  - Front Microbiol. 2021 May 4;12:586886. doi: 10.3389/fmicb.2021.586886. 
      eCollection 2021.