PMID- 34017413
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210523
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 4
DP  - 2021
TI  - Correlation of CD3+/CD4+, and serum CK-18 fragment levels with glucose and lipid 
      metabolism in elderly type 2 diabetes patients with nonalcoholic fatty liver 
      disease.
PG  - 2546-2554
AB  - OBJECTIVE: To test the correlation of helper T lymphocytes (CD3+/CD4+), and 
      cytokeratin 18 fragment (CK-18) with glucose and lipid metabolism in elderly 
      patients with type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver 
      disease (NAFLD). METHODS: A total of 108 patients with T2DM hospitalized in 
      Geriatrics, Taizhou People's Hospital from August 2019 to December 2020 were 
      obtained and grouped into' Non-NAFLD group (58 patients) and NAFLD group (50 
      patients) according to the patients' conditions. Another 50 healthy people were 
      obtained as the control group (CG). The BMI was tested, and the elbow venous 
      blood was collected. The indexes of blood glucose, liver and kidney function 
      (ALT, AST, creatinine, urea nitrogen), blood lipid (triglyceride, total 
      cholesterol, low density lipoprotein cholesterol, high density lipoprotein 
      cholesterol) and blood uric acid were detected. CD3+/CD4+ in elbow venous blood 
      was tested using flow cytometry, and CK-18 was tested using ELISA. Pearson 
      correlation coefficient was applied to test the correlation of CD3+/CD4+, CK-18 
      with glucose and lipid metabolism in NAFLD group. RESULTS: Compared with the CG, 
      CK-18 in the other two groups were elevated, and CK-18 in the NAFLD group were 
      elevated compared to the Non-NAFLD group. Compared with the CG, CD3+ and CD4+ in 
      the other two groups were decreased, and CD3+ and CD4+ in the NAFLD group 
      decreased compared to the Non-NAFLD group. Correlation analysis revealed that 
      both CD3+ and CD4+ had a negative correlation with FPG, HbA1C, FINS, HOMA-IR, TG, 
      TC, HDL and LDL, while CK-18 had a positive correlation with these indexes. ROC 
      curve revealed that the AUC values of CK-18, CD3+ and CD4+ for NAFLD in elderly 
      T2DM patients were 0.875, 0.867, and 0.871, respectively. Logistic regression 
      analysis revealed that FINS, HOMA-IR, CK-18, CD3+ and CD4+ were all related 
      factors leading to NAFLD in elderly T2DM patients. CONCLUSION: CD3+/CD4+, and 
      CK-18 were correlated with glucose and lipid metabolism in elderly T2DM patients 
      with NAFLD. They may be related to the development of T2DM and NAFLD, and these 
      indexes can be used as biological diagnostic indicators for elderly T2DM patients 
      with NAFLD.
CI  - AJTR Copyright (c) 2021.
FAU - Zhao, Chunzhi
AU  - Zhao C
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Lou, Fangyong
AU  - Lou F
AD  - Department of Orthopaedics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Li, Xing
AU  - Li X
AD  - Department of Endocrinology, Second Hospital of Shanxi Medical University Taiyuan 
      030000, Shanxi Province, China.
FAU - Ma, Jianhua
AU  - Ma J
AD  - Department of Endocrinology, Nanjing Hospital Affiliated to Nanjing Medical 
      University Nanjing 210006, Jiangsu Province, China.
FAU - Zhu, Zhengtai
AU  - Zhu Z
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Li, Hua
AU  - Li H
AD  - Department of General Practice, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Zhai, Yuefang
AU  - Zhai Y
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Zhang, Qing
AU  - Zhang Q
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Liu, Zhe
AU  - Liu Z
AD  - Department of Geriatrics, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
FAU - Xiao, Shujun
AU  - Xiao S
AD  - Department of General Practice, Taizhou People's Hospital Taizhou 225300, Jiangsu 
      Province, China.
LA  - eng
PT  - Journal Article
DEP - 20210415
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8129350
OTO - NOTNLM
OT  - CD3+/CD4+
OT  - Type 2 diabetes mellitus
OT  - cytokeratin 18 fragment
OT  - glucose and lipid metabolism
OT  - nonalcoholic fatty liver disease
COIS- None.
EDAT- 2021/05/22 06:00
MHDA- 2021/05/22 06:01
PMCR- 2021/04/15
CRDT- 2021/05/21 06:31
PHST- 2020/12/24 00:00 [received]
PHST- 2021/01/14 00:00 [accepted]
PHST- 2021/05/21 06:31 [entrez]
PHST- 2021/05/22 06:00 [pubmed]
PHST- 2021/05/22 06:01 [medline]
PHST- 2021/04/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Apr 15;13(4):2546-2554. eCollection 2021.