PMID- 34019587
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20211026
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 5
DP  - 2021
TI  - Interleukin-1beta-induced matrix metalloproteinase-3 via ERK1/2 pathway to promote 
      mesenchymal stem cell migration.
PG  - e0252163
LID - 10.1371/journal.pone.0252163 [doi]
LID - e0252163
AB  - Human umbilical cord Wharton's jelly derived mesenchymal stem cells (hUCMSCs), a 
      source of cell therapy, have received a great deal of attention due to their 
      homing or migrating ability in response to signals emanating from damaged sites. 
      It has been found that IL-1beta possesses the ability to induce the expression of 
      matrix metalloproteinase-3 (MMP-3) in bone marrow MSCs. MMP-3 is involved in cell 
      migration in various types of cells, including glioblastoma, vascular smooth 
      muscle, and adult neural progenitor cells. In this study, we proposed that IL-1beta 
      influences hUCMSCs migration involving MMP-3. The expression level of MMP-3 in 
      IL-1beta-induced hUCMSCs was verified using cDNA microarray analysis, quantitative 
      real-time PCR, ELISA and Western blot. Wound-healing and trans-well assay were 
      used to investigate the cell migration and invasion ability of IL-1beta-treated 
      hUCMSCs. In addition, we pre-treated hUCMSCs with interleukin-1 receptor 
      antagonist, MMP-3 inhibitors (ALX-260-165, UK 356618), or transfected with MMP-3 
      siRNA to confirm the role of MMP3 in IL-1beta-induced cell migration. Our results 
      showed that IL-1beta induced MMP-3 expression is related to the migration of 
      hUCMSCs. Moreover, extracellular signal-regulated protein kinases 1 and 2 
      (ERK1/2) inhibitor U0126, p38 inhibitor SB205380, JNK inhibitor SP600125 and Akt 
      inhibitor GSK 690693 decreased IL-1beta-induced MMP-3 mRNA and protein expression. 
      The migration and invasion ability analyses showed that these inhibitors 
      attenuated the IL-1beta-induced migration and invasion ability of hUCMSCs. In 
      conclusion, we have found that IL-1beta induces the expression of MMP-3 through 
      ERK1/2, JNK, p38 MAPK and Akt signaling pathways to enhance the migration of 
      hUCMSCs. These results provide further understanding of the mechanisms in 
      IL-1beta-induced hUCMSCs migration to injury sites.
FAU - Chang, Chun-Hao
AU  - Chang CH
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
FAU - Lin, Yun-Li
AU  - Lin YL
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
FAU - Tyan, Yeu-Sheng
AU  - Tyan YS
AD  - Department of Medical Imaging, Chung Shan Medical University Hospital, Taichung, 
      Taiwan, ROC.
FAU - Chiu, Yun-Hsuan
AU  - Chiu YH
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
FAU - Liang, Ya-Han
AU  - Liang YH
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
FAU - Chen, Chie-Pein
AU  - Chen CP
AD  - Division of High Risk Pregnancy, Mackay Memorial Hospital, Taipei, Taiwan, ROC.
FAU - Wu, Jiahn-Chun
AU  - Wu JC
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
FAU - Wang, Hwai-Shi
AU  - Wang HS
AUID- ORCID: 0000-0003-1818-0970
AD  - Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming 
      Chiao Tung University, Taipei, Taiwan, ROC.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210521
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Interleukin-1beta)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-1beta/*pharmacology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Mesenchymal Stem Cells/*drug effects/*metabolism
MH  - Signal Transduction/drug effects
MH  - Wound Healing/drug effects
PMC - PMC8139494
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/05/22 06:00
MHDA- 2021/10/27 06:00
PMCR- 2021/05/21
CRDT- 2021/05/21 17:19
PHST- 2021/01/11 00:00 [received]
PHST- 2021/05/11 00:00 [accepted]
PHST- 2021/05/21 17:19 [entrez]
PHST- 2021/05/22 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2021/05/21 00:00 [pmc-release]
AID - PONE-D-21-01063 [pii]
AID - 10.1371/journal.pone.0252163 [doi]
PST - epublish
SO  - PLoS One. 2021 May 21;16(5):e0252163. doi: 10.1371/journal.pone.0252163. 
      eCollection 2021.