PMID- 3402029
OWN - NLM
STAT- MEDLINE
DCOM- 19880909
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 9
IP  - 8
DP  - 1988 Aug
TI  - Comparison of mutagenesis induced in single- and double-stranded M13 viral DNA by 
      treatment with N-hydroxy-2-aminofluorene.
PG  - 1337-45
AB  - The specificity of mutagenesis in single-stranded and its complementary 
      double-stranded DNA of the bacteriophage M13mp8 induced by 
      N-hydroxy-2-aminofluorene (N-OH-AF) was analyzed after transfection into its 
      bacterial host Escherichia coli, strain JM103. In this forward mutation assay, 
      randomly modified DNA with increasing levels of aminofluorene (AF) guanine 
      adducts was transfected into competent host JM103 cells with or without prior 
      induction of SOS functions in the host cells. These cells were then screened for 
      mutants of the marker enzyme, beta-galactosidase, on a selective medium. In this 
      assay, the mutation frequency was increased up to 10-fold in host cells with 
      induced SOS functions as compared to the control host cells. Transfection of 
      AF-substituted single-stranded DNA gave a 2.5-fold higher mutation frequency as 
      compared to the double-stranded form at similar levels of AF modification and 
      plaque-forming efficiency. DNA sequence analysis of the mutants showed that 
      AF-modified single- and double-stranded DNA induced base substitutions (52-55%), 
      large deletions (i.e. greater than 300 bp, 25-30%) and frameshifts (16-18%). The 
      mutation sites for 73 base substitutions and frameshifts examined within a 
      limited DNA sequence of 120 bases (6280-6400) were different in single- and 
      double-stranded DNAs. A possible 'hotspot' for base substitutions within one of 
      the two GGCG sequences has also been identified in single-stranded but not 
      double-stranded DNA.
FAU - Gupta, P K
AU  - Gupta PK
AD  - Department of Chemical Carcinogenesis, Michigan Cancer Foundation, Detroit 48201.
FAU - Lee, M S
AU  - Lee MS
FAU - King, C M
AU  - King CM
LA  - eng
GR  - CA23386/CA/NCI NIH HHS/United States
GR  - CA38844/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA, Viral)
RN  - 0 (Fluorenes)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
SB  - IM
MH  - Base Sequence
MH  - DNA Repair
MH  - DNA, Single-Stranded/*drug effects
MH  - DNA, Viral/analysis/*drug effects/metabolism
MH  - Fluorenes/metabolism/*toxicity
MH  - *Mutation
MH  - Transfection
MH  - Viral Plaque Assay
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 10.1093/carcin/9.8.1337 [doi]
PST - ppublish
SO  - Carcinogenesis. 1988 Aug;9(8):1337-45. doi: 10.1093/carcin/9.8.1337.