PMID- 34024
OWN - NLM
STAT- MEDLINE
DCOM- 19790523
LR  - 20190710
IS  - 0022-3549 (Print)
IS  - 0022-3549 (Linking)
VI  - 68
IP  - 3
DP  - 1979 Mar
TI  - Facile in vitro method for screening inhibitors of IgE binding to mast cells.
PG  - 353-7
AB  - A method for rapidly testing large numbers of chemical structures as potential 
      modulators of the interaction between immunoglobulin E (IgE) and its specific 
      receptors on rat peritoneal mast cells is described. IgE, isolated from the 
      ascitic fluid of a transplantable rat IgE immunocytoma, is labeled with 
      iodine-125 under mild conditions employing the Bolton--Hunter reagent. The 
      antibody is incubated with mixed periotoneal cells at 37 degrees, and the 
      cell-bound IgE is separated from unbound label by sedimentation through an 8% 
      sucrose--polymer solution in microsediment tubes. Optimal conditions for the 
      interaction of 3 nM IgE with 3 X 10(5) mast cells in 150 mul are: incubation 
      time, 2 hr; pH, 6.5--7.0; and ionic strength, equivalent to 150 mM NaCl. Mixed 
      peritoneal cells bind IgE with an affinity equal to that of purified mast cells. 
      Human IgE pentapeptide III and several antiallergic agents do not compete with 
      rat IgE in this assay.
FAU - Coutts, S M
AU  - Coutts SM
FAU - Reid, D M
AU  - Reid DM
FAU - Weinryb, I
AU  - Weinryb I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pharm Sci
JT  - Journal of pharmaceutical sciences
JID - 2985195R
RN  - 0 (Immunoglobulin Fragments)
RN  - 0 (Myeloma Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/cytology
MH  - Binding Sites
MH  - Hydrogen-Ion Concentration
MH  - Hypersensitivity/drug therapy
MH  - Immunoglobulin E/*metabolism/pharmacology
MH  - Immunoglobulin Fragments/pharmacology
MH  - In Vitro Techniques
MH  - Mast Cells/drug effects/*immunology
MH  - Myeloma Proteins/pharmacology
MH  - Protein Binding/drug effects
MH  - Rats
EDAT- 1979/03/01 00:00
MHDA- 1979/03/01 00:01
CRDT- 1979/03/01 00:00
PHST- 1979/03/01 00:00 [pubmed]
PHST- 1979/03/01 00:01 [medline]
PHST- 1979/03/01 00:00 [entrez]
AID - S0022-3549(15)42511-0 [pii]
AID - 10.1002/jps.2600680326 [doi]
PST - ppublish
SO  - J Pharm Sci. 1979 Mar;68(3):353-7. doi: 10.1002/jps.2600680326.