PMID- 34025617
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210526
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 12
DP  - 2021
TI  - A Chimeric Cationic Peptide Composed of Human beta-Defensin 3 and Human beta-Defensin 4 
      Exhibits Improved Antibacterial Activity and Salt Resistance.
PG  - 663151
LID - 10.3389/fmicb.2021.663151 [doi]
LID - 663151
AB  - Human beta-defensins (hBDs) play an important role in the host defense against 
      various microbes, showing different levels of antibacterial activity and salt 
      resistance in vitro. It is of interest to investigate whether can chimeric hBD 
      analogs enhanced antibacterial activity and salt resistance. In this study, we 
      designed a chimeric human defensin, named H4, by combining sequences of human 
      beta-defensin-3 (hBD-3) and human beta-defensin-4 (hBD-4), then evaluated its 
      antibacterial activity, salt resistance, and cytotoxic effects. The result showed 
      that the antibacterial activity of H4 against most tested strains, including 
      Klebsiella pneumonia, Enterococcus faecalis, Staphyloccocus aureus, Escherichia 
      coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Acinetobacter baumannii 
      was significantly improved compared to that of hBD-3 and hBD-4. Notably, H4 
      exhibited significantly better antibacterial activity against multidrug resistant 
      isolate A. baumannii MDR-ZJ06 than commonly used antibiotics. Chimeric H4 still 
      showed more than 80% antibacterial activity at high salt concentration (150 muM), 
      which proves its good salt tolerance. The cytotoxic effect assay showed that the 
      toxicity of H4 to Hela, Vero, A549 cells and erythrocytes at a low dose (<10 
      mug/ml) was similar to that of hBD-3 and hBD-4. In conclusion, given its broad 
      spectrum of antibacterial activity and high salt resistance, chimeric H4 could 
      serve as a promising template for new therapeutic antimicrobial agents.
CI  - Copyright (c) 2021 Yu, Ning, Xue, Huang, Guo, Li, Yang, Luo, Sun, Li, Wang, He, 
      Cheng, Zhang and Wang.
FAU - Yu, Wenjing
AU  - Yu W
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Ning, Nianzhi
AU  - Ning N
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Xue, Ying
AU  - Xue Y
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
AD  - College of Life Science, Ludong University, Yantai, China.
FAU - Huang, Yanyu
AU  - Huang Y
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Guo, Feng
AU  - Guo F
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Li, Tao
AU  - Li T
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Yang, Boning
AU  - Yang B
AD  - Department of Orthopedics, Henan University People's Hospital, Zhengzhou, China.
FAU - Luo, Deyan
AU  - Luo D
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Sun, Yakun
AU  - Sun Y
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Li, Zhan
AU  - Li Z
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Wang, Jianxin
AU  - Wang J
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - He, Zhili
AU  - He Z
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
FAU - Cheng, Shiwei
AU  - Cheng S
AD  - College of Life Science, Ludong University, Yantai, China.
FAU - Zhang, Xingxiao
AU  - Zhang X
AD  - College of Life Science, Ludong University, Yantai, China.
FAU - Wang, Hui
AU  - Wang H
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20210507
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8137984
OTO - NOTNLM
OT  - antibacterial activity
OT  - chimeric human defensin
OT  - human beta-defensins
OT  - multidrug resistant
OT  - salt resistance
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/05/25 06:00
MHDA- 2021/05/25 06:01
PMCR- 2021/05/07
CRDT- 2021/05/24 08:04
PHST- 2021/02/02 00:00 [received]
PHST- 2021/04/14 00:00 [accepted]
PHST- 2021/05/24 08:04 [entrez]
PHST- 2021/05/25 06:00 [pubmed]
PHST- 2021/05/25 06:01 [medline]
PHST- 2021/05/07 00:00 [pmc-release]
AID - 10.3389/fmicb.2021.663151 [doi]
PST - epublish
SO  - Front Microbiol. 2021 May 7;12:663151. doi: 10.3389/fmicb.2021.663151. 
      eCollection 2021.