PMID- 34028142
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20211130
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 131
IP  - 6
DP  - 2021 Dec
TI  - Grapevine rootstocks drive the community structure of arbuscular mycorrhizal 
      fungi in New Zealand vineyards.
PG  - 2941-2956
LID - 10.1111/jam.15160 [doi]
AB  - AIM: Arbuscular mycorrhizal fungi (AMF) are often regarded as non-specific 
      symbionts, but some AMF communities show host preference in various ecosystems 
      including vineyards. Grapevine plants are very responsive to AMF colonization. 
      Although these fungi have potentially significant applications for sustainable 
      agricultural ecosystems, there is a gap in knowledge regarding AMF-grapevine 
      interactions worldwide and especially in New Zealand. This study focused on 
      identifying AMF taxa colonizing grapevines in New Zealand vineyards and 
      investigated the effect of grapevine rootstocks on AMF community diversity and 
      composition. METHODS AND RESULTS: Denaturing gradient gel electrophoresis (DGGE) 
      and trap cultures were used to characterize the AMF communities. Grapevine roots 
      from three vineyards and nine rootstocks were analysed by DGGE and used in trap 
      cultures for AMF recovery. Trap cultures allowed the recovery of six AMF spore 
      morphotypes that belonged to Ambispora sp., Claroideoglomus sp., Funneliformis 
      sp. and Glomus sp. Bands excised, reamplified and sequenced from the DGGE were 
      assigned to Glomus sp., Rhizophagus sp. and Claroideoglomus sp. The AMF community 
      analyses demonstrated that rootstock significantly (P < 0.05) influenced the AMF 
      community composition in all sites. CONCLUSIONS: The study showed that for a 
      comprehensive identification of AMF, both results from trap culture and molecular 
      work were needed and that the rootstock cultivar was the main driver of the 
      arbuscular mycorrhizal community colonizing the roots. SIGNIFICANCE AND IMPACT OF 
      THE STUDY: This study provides a firm foundation for future research exploring 
      the beneficial use of AMF in enhancing grapevine production and sustainability.
CI  - (c) 2021 The Society for Applied Microbiology.
FAU - Moukarzel, R
AU  - Moukarzel R
AUID- ORCID: 0000-0001-5788-4327
AD  - Lincoln University, Lincoln, Canterbury, New Zealand.
FAU - Ridgway, H J
AU  - Ridgway HJ
AD  - The New Zealand Institute for Plant and Food Research Ltd, Private Bag 4704, 
      Christchurch, New Zealand.
FAU - Guerin-Laguette, A
AU  - Guerin-Laguette A
AD  - Mycotree C/-Southern Woods Nursery, Christchurch, Canterbury, New Zealand.
FAU - Jones, E E
AU  - Jones EE
AUID- ORCID: 0000-0002-1879-4537
AD  - Lincoln University, Lincoln, Canterbury, New Zealand.
LA  - eng
GR  - New Zealand Institute for Plant and Food Research Limited/
GR  - Lincoln University/
PT  - Journal Article
DEP - 20210607
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
SB  - IM
MH  - Ecosystem
MH  - Farms
MH  - Fungi
MH  - *Mycorrhizae/genetics
MH  - New Zealand
MH  - Plant Roots
MH  - Soil Microbiology
OTO - NOTNLM
OT  - DGGE
OT  - diversity
OT  - fungi
OT  - spores
OT  - symbiosis
OT  - trap culture
EDAT- 2021/05/25 06:00
MHDA- 2021/12/01 06:00
CRDT- 2021/05/24 10:16
PHST- 2021/05/02 00:00 [revised]
PHST- 2021/02/25 00:00 [received]
PHST- 2021/05/20 00:00 [accepted]
PHST- 2021/05/25 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2021/05/24 10:16 [entrez]
AID - 10.1111/jam.15160 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2021 Dec;131(6):2941-2956. doi: 10.1111/jam.15160. Epub 2021 
      Jun 7.