PMID- 34028740
OWN - NLM
STAT- MEDLINE
DCOM- 20210810
LR  - 20240124
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2299
DP  - 2021
TI  - Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices.
PG  - 139-145
LID - 10.1007/978-1-0716-1382-5_10 [doi]
AB  - Fibroblast-to-myofibroblast transdifferentiation and the acquisition of a 
      senescent phenotype are hallmarks of fibrotic diseases. The study of the 
      localization of senescent myofibroblasts as well as their interactions with other 
      cell types in the fibrotic tissue has been hindered by the lack of methods to 
      detect these cells in vivo. Here, we describe methods to detect tissue 
      localization of senescent myofibroblasts in precision-cut lung slices (PCLS) by 
      combining beta-galactosidase staining with immunofluorescence techniques.
FAU - Cruz, Tamara
AU  - Cruz T
AD  - Division of Pulmonary, Allergy, and Critical Care Medicine, University of 
      Pittsburgh School of Medicine, Pittsburgh, PA, USA.
FAU - Mora, Ana L
AU  - Mora AL
AD  - Division of Pulmonary, Allergy, and Critical Care Medicine, University of 
      Pittsburgh School of Medicine, Pittsburgh, PA, USA.
AD  - Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, 
      USA.
FAU - Rojas, Mauricio
AU  - Rojas M
AD  - Division of Pulmonary, Allergy, and Critical Care Medicine, University of 
      Pittsburgh School of Medicine, Pittsburgh, PA, USA. rojasm@upmc.edu.
AD  - Aging Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, 
      USA. rojasm@upmc.edu.
LA  - eng
GR  - P50 AR060780/AR/NIAMS NIH HHS/United States
GR  - R01 HL149825/HL/NHLBI NIH HHS/United States
GR  - U01 HL145550/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cell Transdifferentiation
MH  - Cellular Senescence
MH  - Fibroblasts/cytology/*metabolism
MH  - Fibrosis
MH  - Humans
MH  - Lung/metabolism/*pathology
MH  - Mice
MH  - Myofibroblasts/cytology/*metabolism
MH  - beta-Galactosidase/metabolism
PMC - PMC8650767
MID - NIHMS1755385
OTO - NOTNLM
OT  - Fibrosis
OT  - Myofibroblasts
OT  - PCLS
OT  - Senescence
OT  - beta-Galactosidase
EDAT- 2021/05/25 06:00
MHDA- 2021/08/11 06:00
PMCR- 2021/12/07
CRDT- 2021/05/24 12:38
PHST- 2021/05/24 12:38 [entrez]
PHST- 2021/05/25 06:00 [pubmed]
PHST- 2021/08/11 06:00 [medline]
PHST- 2021/12/07 00:00 [pmc-release]
AID - 10.1007/978-1-0716-1382-5_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2299:139-145. doi: 10.1007/978-1-0716-1382-5_10.