PMID- 34028760
OWN - NLM
STAT- MEDLINE
DCOM- 20210810
LR  - 20210810
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2299
DP  - 2021
TI  - Decellularized Human Lung Scaffolds as Complex Three-Dimensional Tissue Culture 
      Models to Study Functional Behavior of Fibroblasts.
PG  - 447-456
LID - 10.1007/978-1-0716-1382-5_30 [doi]
AB  - In vitro culturing of cells in two-dimensional (2D) environments is a widespread 
      used methodology in biomedical research. Most commonly, cells are cultured on 
      artificial plastic dish surfaces, which lead to abnormal functional behaviors, as 
      plastic does not reflect the native microenvironment found in vivo or in situ. 
      Therefore, a multitude of three-dimensional (3D) cell culture systems were 
      developed in the past years, which aim to bridge the gap between 2D cell culture 
      dishes and the in vivo situation. One of the more recent development in the 
      field, the generation of viable precision-cut tissue slices from various organs 
      emerged as an exciting approach to study complex interactions and biological 
      processes ex vivo in 3D. Decellularization of such tissue slices leads to the 
      removal of all functional cells, and leaves behind a scaffold of extracellular 
      matrix (ECM), which closely recapitulates the molecular composition, mechanical 
      properties, topology, and microarchitecture of native ECM. Subsequently, 
      decellularized precision-cut lung slices (PCLS), also called 3D lung tissue 
      culture (3D-LTCs), can be successfully reseeded with a variety of cell types, 
      including fibroblasts, which attach to and engraft into the matrix. Here, we 
      describe the generation of PCLS from resected human lung tissue and their 
      decellularization and recellularization with primary human fibroblasts. This 
      novel 3D tissue culture model allows for various functional studies of fibroblast 
      behavior on native ECM composition and topology.
FAU - Burgstaller, Gerald
AU  - Burgstaller G
AD  - Institute of Lung Biology and Disease (ILBD) and Comprehensive Pneumology Center 
      (CPC), Helmholtz Zentrum Munchen, Member of the German Center for Lung Research 
      (DZL), Munich, Germany. gerald.burgstaller@helmholtz-muenchen.de.
AD  - CPC-M bioArchive, Helmholtz Zentrum Munchen, Comprehensive Pneumology Center 
      Munich DZL/CPC-M, Munchen, Germany. gerald.burgstaller@helmholtz-muenchen.de.
FAU - Gerckens, Michael
AU  - Gerckens M
AD  - Institute of Lung Biology and Disease (ILBD) and Comprehensive Pneumology Center 
      (CPC), Helmholtz Zentrum Munchen, Member of the German Center for Lung Research 
      (DZL), Munich, Germany.
AD  - CPC-M bioArchive, Helmholtz Zentrum Munchen, Comprehensive Pneumology Center 
      Munich DZL/CPC-M, Munchen, Germany.
FAU - Eickelberg, Oliver
AU  - Eickelberg O
AD  - Division of Pulmonary, Allergy, and Critical Care Medicine, Department of 
      Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
FAU - Konigshoff, Melanie
AU  - Konigshoff M
AD  - CPC-M bioArchive, Helmholtz Zentrum Munchen, Comprehensive Pneumology Center 
      Munich DZL/CPC-M, Munchen, Germany.
AD  - Division of Pulmonary, Allergy, and Critical Care Medicine, Department of 
      Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
AD  - Comprehensive Pneumology Center (CPC), Research Unit Lung Repair and 
      Regeneration, Helmholtz Zentrum Munchen, Member of the German Center for Lung 
      Research (DZL), Munich, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cells, Cultured
MH  - Extracellular Matrix/metabolism
MH  - Fibroblasts/*cytology/metabolism
MH  - Humans
MH  - Lung/*cytology/metabolism
MH  - Tissue Culture Techniques/*methods
MH  - Tissue Engineering
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - 3D cell culture models
OT  - 3D-LTCs
OT  - Decellularization
OT  - Fibroblasts
OT  - Human ex vivo models
OT  - Myofibroblasts
OT  - PCLS
OT  - Pharmacological testing
OT  - Precision-cut lung slices
OT  - Recellularization
OT  - Tissue engineering
OT  - Transdifferentiation
EDAT- 2021/05/25 06:00
MHDA- 2021/08/11 06:00
CRDT- 2021/05/24 12:38
PHST- 2021/05/24 12:38 [entrez]
PHST- 2021/05/25 06:00 [pubmed]
PHST- 2021/08/11 06:00 [medline]
AID - 10.1007/978-1-0716-1382-5_30 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2299:447-456. doi: 10.1007/978-1-0716-1382-5_30.