PMID- 34030495 OWN - NLM STAT- MEDLINE DCOM- 20220420 LR - 20230302 IS - 1097-6817 (Electronic) IS - 0194-5998 (Print) IS - 0194-5998 (Linking) VI - 166 IP - 3 DP - 2022 Mar TI - Tumor-Infiltrating Lymphocytes in Patients With Advanced Laryngeal Cancer Undergoing Bioselection. PG - 498-505 LID - 10.1177/01945998211013765 [doi] AB - OBJECTIVE: Bioselection to assess tumor response after induction chemotherapy has been introduced as an alternative treatment strategy to total laryngectomy for patients with advanced larynx squamous cell carcinoma (LSCC). Tumor-infiltrating lymphocytes (TILs) have proven to serve as prognostic biomarkers in head and neck cancer but have not been evaluated as a way to select patients for treatment paradigms. The aim of this study is to evaluate the role of pretreatment TILs in patients with advanced LSCC undergoing the bioselection paradigm. STUDY DESIGN: Retrospective study. SETTING: Tertiary care hospital. METHODS: Patients with advanced LSCC treated with bioselection and available tissue were included (N = 76). Patients were stratified into CD8-low and CD8-high cohorts by using the median TIL count. Kaplan-Meier survival analysis and multivariate cox regression were performed with SPSS version 26 (IBM). RESULTS: After controlling for tobacco use, tumor site, and stage, a high CD8 TIL count was an independent predictor of improved 5-year disease-specific survival (hazard ratio, 0.17 [95% CI, 0.03-0.84]; P = .03). CD8 TIL counts did not predict response to induction chemotherapy; however, subgroup analysis of patients treated with chemoradiation therapy revealed that CD8 TIL count was significantly associated with degree of response (P = .012). CONCLUSION: These findings support prior data published by our group showing that TILs are predictive of disease-specific survival in patients with head and neck cancer. CD8 TIL counts were significantly associated with degree of clinical response after induction chemotherapy. These results suggest that pretreatment assessment of tumor-infiltrating CD8 cells could be useful in selecting patients. FAU - Heft Neal, Molly E AU - Heft Neal ME AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Smith, Joshua D AU - Smith JD AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Birkeland, Andrew C AU - Birkeland AC AD - Department of Otolaryngology-Head and Neck Surgery, University of California Davis, Sacramento, California, USA. FAU - Haring, Catherine T AU - Haring CT AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Chinn, Steven B AU - Chinn SB AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Shuman, Andrew G AU - Shuman AG AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Casper, Keith A AU - Casper KA AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Malloy, Kelly M AU - Malloy KM AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Stucken, Chaz L AU - Stucken CL AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Mclean, Scott A AU - Mclean SA AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Rosko, Andrew J AU - Rosko AJ AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Mierzwa, Michelle L AU - Mierzwa ML AD - Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan, USA. FAU - Shah, Jennifer AU - Shah J AD - Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan, USA. FAU - Schonewolf, Caitlin AU - Schonewolf C AD - Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan, USA. FAU - Swiecicki, Paul L AU - Swiecicki PL AD - Division of Hematology and Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA. FAU - Worden, Francis P AU - Worden FP AD - Division of Hematology and Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA. FAU - Wolf, Gregory T AU - Wolf GT AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Bradford, Carol R AU - Bradford CR AD - Department of Otolaryngology-Head and Neck Surgery, The Ohio State University, Columbus, Ohio, USA. FAU - Prince, Mark E P AU - Prince MEP AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Brenner, J Chad AU - Brenner JC AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. FAU - Spector, Matthew E AU - Spector ME AD - Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA. LA - eng GR - T32 DC005356/DC/NIDCD NIH HHS/United States GR - U01 DE029255/DE/NIDCR NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20210525 PL - England TA - Otolaryngol Head Neck Surg JT - Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery JID - 8508176 SB - IM MH - *Head and Neck Neoplasms/pathology MH - Humans MH - *Laryngeal Neoplasms/surgery MH - *Larynx/pathology MH - Lymphocytes, Tumor-Infiltrating MH - Prognosis MH - Retrospective Studies MH - Squamous Cell Carcinoma of Head and Neck/pathology/therapy PMC - PMC8613299 MID - NIHMS1734885 OTO - NOTNLM OT - induction selection OT - larynx cancer OT - tumor-infiltrating lymphocytes COIS- Declaration of Conflicting Interests: The authors declares that there are no conflicts of interest. Disclosures: There are no conflicts of interests to disclose. EDAT- 2021/05/26 06:00 MHDA- 2022/04/21 06:00 PMCR- 2023/03/01 CRDT- 2021/05/25 05:33 PHST- 2021/05/26 06:00 [pubmed] PHST- 2022/04/21 06:00 [medline] PHST- 2021/05/25 05:33 [entrez] PHST- 2023/03/01 00:00 [pmc-release] AID - 10.1177/01945998211013765 [doi] PST - ppublish SO - Otolaryngol Head Neck Surg. 2022 Mar;166(3):498-505. doi: 10.1177/01945998211013765. Epub 2021 May 25.