PMID- 34032966
OWN - NLM
STAT- MEDLINE
DCOM- 20220217
LR  - 20220217
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 53
IP  - 4
DP  - 2021 Aug
TI  - Knockdown of ANXA10 inhibits proliferation and promotes apoptosis of papillary 
      thyroid carcinoma cells by down-regulating TSG101 thereby inactivating the 
      MAPK/ERK signaling pathway.
PG  - 429-440
LID - 10.1007/s10863-021-09902-7 [doi]
AB  - Annexin A10 (ANXA10) is a member of annexin A and has been reported to highly 
      express in papillary thyroid carcinoma (PTC) tissues. Tumor susceptibility gene 
      101 (TSG101) also plays a role in PTC and is predicted to bind to ANXA10. This 
      study intended to investigate whether ANXA10 could regulate PTC via binding to 
      ANXA10. The expression of ANXA10 and TSG101 in normal thyroid follicular 
      epithelial cell line and several PTC cell lines was analyzed using RT-qPCR and 
      western blotting assays. Subsequently, PTC cell line BCPAP was silenced with 
      ANXA10 followed by TSG101 overexpression or not, and then cell proliferation, 
      apoptosis and mitogen-activated protein kinase (MAPK) signaling expression were 
      assessed via MTT, colony formation, immunofluorescence staining, Tunel staining 
      and western blotting assays. Besides, the interaction between ANXA10 and TSG101 
      was validated using Co-immunoprecipitation assay. ANXA10 and TSG101 expressions 
      were up-regulated in PTC cell lines. ANXA10 silence inhibited proliferation, 
      promoted apoptosis and inactivated MAPK/ extracellular regulated protein kinases 
      (ERK) signaling pathway of BCPAP cells. Additionally, ANXA10 could bind to TSG101 
      and regulate its expression. However, the above effects of ANXA10 silence on 
      BCPAP cells were all blocked by TSG101 overexpression. ANXA10 inhibited 
      proliferation and promoted apoptosis of PTC cells via binding to TSG101, and 
      these actions may depend on down-regulating MAPK/ERK pathway expression.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Wei, Tianjun
AU  - Wei T
AD  - Department of Ultrasonography, The First Affiliated Hospital of Wannan Medical 
      College, NO.2 zheshan west Road, Wuhu City, 241001, Anhui province, China.
FAU - Zhu, Xiangming
AU  - Zhu X
AD  - Department of Ultrasonography, The First Affiliated Hospital of Wannan Medical 
      College, NO.2 zheshan west Road, Wuhu City, 241001, Anhui province, China. 
      zhuxiangming06@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210525
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - 0 (ANXA10 protein, human)
RN  - 0 (Annexins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Transcription Factors)
RN  - 0 (Tsg101 protein)
SB  - IM
MH  - Annexins/genetics/*metabolism
MH  - Apoptosis/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/physiology
MH  - DNA-Binding Proteins/*metabolism
MH  - Down-Regulation
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - Humans
MH  - *MAP Kinase Signaling System
MH  - Signal Transduction
MH  - Thyroid Cancer, Papillary/genetics/*metabolism/pathology
MH  - Thyroid Neoplasms/genetics/*metabolism/pathology
MH  - Transcription Factors/*metabolism
MH  - Transfection
OTO - NOTNLM
OT  - Annexin A10
OT  - Papillary thyroid carcinoma
OT  - Thyroid cancer
OT  - Tumor susceptibility gene 101
EDAT- 2021/05/26 06:00
MHDA- 2022/02/19 06:00
CRDT- 2021/05/25 14:03
PHST- 2021/04/02 00:00 [received]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/05/26 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2021/05/25 14:03 [entrez]
AID - 10.1007/s10863-021-09902-7 [pii]
AID - 10.1007/s10863-021-09902-7 [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2021 Aug;53(4):429-440. doi: 10.1007/s10863-021-09902-7. 
      Epub 2021 May 25.