PMID- 34033869
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20220531
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 467
DP  - 2021 Jul 15
TI  - Characterization of Hevin (SPARCL1) Immunoreactivity in Postmortem Human Brain 
      Homogenates.
PG  - 91-109
LID - S0306-4522(21)00256-6 [pii]
LID - 10.1016/j.neuroscience.2021.05.017 [doi]
AB  - Hevin is a matricellular glycoprotein that plays important roles in neural 
      developmental processes such as neuronal migration, synaptogenesis and synaptic 
      plasticity. In contrast to other matricellular proteins whose expression 
      decreases when development is complete, hevin remains highly expressed, 
      suggesting its involvement in adult brain function. In vitro studies have shown 
      that hevin can have different post-translational modifications. However, the 
      glycosylation pattern of hevin in the human brain remains unknown, as well as its 
      relative distribution and localization. The present study provides the first 
      thorough characterization of hevin protein expression by Western blot in 
      postmortem adult human brain. Our results demonstrated two major specific 
      immunoreactive bands for hevin: an intense band migrating around 130 kDa, and a 
      band migrating around 100 kDa. Biochemical assays revealed that both hevin bands 
      have a different glycosylation pattern. Subcellular fractionation showed greater 
      expression in membrane-enriched fraction than in cytosolic preparation, and a 
      higher expression in prefrontal cortex (PFC) compared to hippocampus (HIP), 
      caudate nucleus (CAU) and cerebellum (CB). We confirmed that a disintegrin and 
      metalloproteinase with thrombospondin motifs 4 (ADAMTS4) and 
      matrixmetalloproteinase 3 (MMP-3) proteases digestion led to an intense double 
      band with similar molecular weight to that described as SPARC-like fragment 
      (SLF). Finally, hevin immunoreactivity was also detected in human astrocytoma, 
      meningioma, cerebrospinal fluid and serum samples, but was absent from any blood 
      cell type.
CI  - Copyright (c) 2021 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Nunez-delMoral, Amaia
AU  - Nunez-delMoral A
AD  - Department of Pharmacology, University of the Basque Country, UPV/EHU, Leioa, 
      Bizkaia, Spain; Centro de Investigacion Biomedica en Red de Salud Mental 
      (CIBERSAM), Spain.
FAU - Brocos-Mosquera, Iria
AU  - Brocos-Mosquera I
AD  - Department of Pharmacology, University of the Basque Country, UPV/EHU, Leioa, 
      Bizkaia, Spain; Centro de Investigacion Biomedica en Red de Salud Mental 
      (CIBERSAM), Spain.
FAU - Vialou, Vincent
AU  - Vialou V
AD  - Sorbonne Universite, Neuroscience Paris Seine - IBPS, Team Neurobiology of 
      Psychiatric Disorders, Paris, France; CNRS UMR8246, Neuroscience Paris Seine - 
      IBPS, Team Neurobiology of Psychiatric Disorders, Paris, France; Inserm U1130, 
      Neuroscience Paris Seine - IBPS, Team Neurobiology of Psychiatric Disorders, 
      Paris, France. Electronic address: vincent.vialou@inserm.fr.
FAU - Callado, Luis F
AU  - Callado LF
AD  - Department of Pharmacology, University of the Basque Country, UPV/EHU, Leioa, 
      Bizkaia, Spain; Centro de Investigacion Biomedica en Red de Salud Mental 
      (CIBERSAM), Spain; BioCruces Bizkaia Health Research Institute, Barakaldo, 
      Bizkaia, Spain.
FAU - Erdozain, Amaia M
AU  - Erdozain AM
AD  - Department of Pharmacology, University of the Basque Country, UPV/EHU, Leioa, 
      Bizkaia, Spain; Centro de Investigacion Biomedica en Red de Salud Mental 
      (CIBERSAM), Spain. Electronic address: amaia_erdozain@ehu.eus.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210524
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Osteonectin)
RN  - 0 (SPARCL1 protein, human)
SB  - IM
MH  - Adult
MH  - Blotting, Western
MH  - Brain/metabolism
MH  - Calcium-Binding Proteins
MH  - Cerebellum/metabolism
MH  - *Extracellular Matrix Proteins/metabolism
MH  - Humans
MH  - Neurogenesis
MH  - *Osteonectin/metabolism
OTO - NOTNLM
OT  - SPARCL1
OT  - Western blot
OT  - hevin
OT  - human brain
OT  - postmortem
EDAT- 2021/05/26 06:00
MHDA- 2021/07/13 06:00
CRDT- 2021/05/25 20:12
PHST- 2021/03/12 00:00 [received]
PHST- 2021/05/12 00:00 [revised]
PHST- 2021/05/13 00:00 [accepted]
PHST- 2021/05/26 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2021/05/25 20:12 [entrez]
AID - S0306-4522(21)00256-6 [pii]
AID - 10.1016/j.neuroscience.2021.05.017 [doi]
PST - ppublish
SO  - Neuroscience. 2021 Jul 15;467:91-109. doi: 10.1016/j.neuroscience.2021.05.017. 
      Epub 2021 May 24.