PMID- 34038780
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1649
DP  - 2021 Jul 19
TI  - Determination of acetylgestagens in animal-derived matrix samples using enhanced 
      matrix removal lipid clean-up in combination with ultra performance liquid 
      chromatography-tandem mass spectrometry.
PG  - 462227
LID - S0021-9673(21)00351-4 [pii]
LID - 10.1016/j.chroma.2021.462227 [doi]
AB  - A robust and confirmative method was established for the determination of six 
      acetylgestagen residues, namely, flurogestone acetate (FGA), megestrol (MA), 
      melengestrol acetate (MGA), chlormadinone acetate (CMA), medroxyprogesterone 
      (MPA), and hydroxyprogesterone acetate (HPA) in animal-derived matrix samples by 
      utilizing enhanced matrix removal lipid (EMR-lipid) clean-up in combination with 
      ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 
      The analytes were extracted with acetonitrile, purified with a EMR-lipid 
      cartridge, and separated with a reversed-phase C18 column. The limit of 
      quantification (S/N >/= 10) for CMA, FGA, HPA, MA, and MGA in all matrices was 
      0.5 ng/g, and for MPA, it was 1.0 ng/g; the limit of detection (S/N >/= 3) for CMA, 
      FGA, HPA, MA, and MGA in all matrices was 0.1 ng/g, and for MPA, it was 0.2 ng/g. 
      The recoveries were between 61.0% and 114.8%, and the relative standard 
      deviations (RSDs) were below 12%. The method was calibrated in a matrix-assisted 
      standard solution in various linear ranges for the analytes and matrices, and the 
      correlation coefficients (R(2)) exceeded 0.99 for all the matrices.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Zhang, Congcong
AU  - Zhang C
AD  - Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards 
      & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 
      Beijing, 100081, China.
FAU - Zhang, Qingyang
AU  - Zhang Q
AD  - Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards 
      & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 
      Beijing, 100081, China.
FAU - Yin, Zenghao
AU  - Yin Z
AD  - Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards 
      & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 
      Beijing, 100081, China.
FAU - Hu, Jian
AU  - Hu J
AD  - Agricultural and Rural Affair Bureau, Fengnan District, Tangshan City, Hebei 
      Province, 063300, China.
FAU - Chen, Gang
AU  - Chen G
AD  - Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards 
      & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 
      Beijing, 100081, China.
FAU - Zheng, Lufei
AU  - Zheng L
AD  - Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards 
      & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 
      Beijing, 100081, China. Electronic address: zhenglufei@caas.cn.
FAU - Ma, Aijin
AU  - Ma A
AD  - School of Food and Health, Beijing Technology and Business University, Beijing, 
      100048, China. Electronic address: maaj@btbu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210507
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Lipids)
RN  - 0 (Progestins)
SB  - IM
MH  - Animals
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Liquid/*methods
MH  - Drug Residues/analysis
MH  - Lipids/*isolation & purification
MH  - Progestins/*analysis/pharmacokinetics
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Acetylgestagens
OT  - Animal-derived matrix sample
OT  - EMR-lipid cartridge
OT  - Liquid chromatography-tandem mass spectrometry
COIS- Declaration of Competing Interest We hereby confirm that this manuscript is our 
      original work and has not been published nor has it been submitted simultaneously 
      elsewhere. We further confirm that all authors have checked the manuscript and 
      have agreed to the submission.
EDAT- 2021/05/27 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/05/26 20:12
PHST- 2021/01/17 00:00 [received]
PHST- 2021/04/26 00:00 [revised]
PHST- 2021/04/30 00:00 [accepted]
PHST- 2021/05/27 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/05/26 20:12 [entrez]
AID - S0021-9673(21)00351-4 [pii]
AID - 10.1016/j.chroma.2021.462227 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Jul 19;1649:462227. doi: 10.1016/j.chroma.2021.462227. Epub 
      2021 May 7.