PMID- 34041764
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20210722
IS  - 1745-4514 (Electronic)
IS  - 0145-8884 (Linking)
VI  - 45
IP  - 7
DP  - 2021 Jul
TI  - Rat plasma protein binding of kaempferol-3-O-rutinoside from Lu'an GuaPian tea 
      and its anti-inflammatory mechanism for cardiovascular protection.
PG  - e13749
LID - 10.1111/jfbc.13749 [doi]
AB  - Previous study found a high content of kaempferol-3-O-rutinoside (KR) in Lu'an 
      GuaPian tea, however, the rat plasma protein binding and mechanism of KR for 
      cardiovascular protection are unclear. Thus, we studied plasma protein binding 
      using ultrafiltration followed by UPLC, and screened its inhibition against 
      LPS-induced inflammation injury in vitro as well as the underlying mechanism by 
      molecular docking and western blot. KR showed over 74% plasma protein binding 
      ratio. Furthermore, KR may act on the toll-like receptor 4 (TLR4) and myeloid 
      differentiation factor 88 (MyD88). In vitro experiments showed that KR decreases 
      the overexpression of TLR4, MyD88, and nuclear factor-kappaB (NF-kappaB), which further 
      validates the molecular docking results, suggesting that KR could block 
      TLR4/MyD88/NF-kappaB signaling. These results indicate that KR could be a potential 
      active agent in the protection of myocardial injury. PRACTICAL APPLICATIONS: 
      Health benefits of tea are largely dependent on the intake of flavonoids. 
      Flavonoids are a group of compounds beneficial to cardiovascular disease and an 
      important part of "functional foods." Lu'an GuaPian tea is mainly produced in 
      Lu'an City, Anhui Province and is one of the top 10 famous teas in China. 
      Kaempferol-3-O-rutinoside in Lu'an GuaPian has good hypoglycemic effect, mainly 
      manifested in a strong inhibition of alpha-glucosidase and alpha-amylase activities. 
      Present study showed that kaempferol-3-O-rutinoside could block TLR4/MyD88/NF-kappaB 
      signaling, suggesting that it could be a potential active agent in the protection 
      of myocardial injury.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Hua, Fang
AU  - Hua F
AD  - Natural Products Laboratory, State Key Laboratory of Tea Plant Biology and 
      Utilization, Anhui Agricultural University, Hefei, China.
AD  - School of Pharmacy, Anhui Xinhua University, Hefei, China.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Department of Integrated Traditional Chinese and Western Medicine, Anhui 
      University of Chinese Medicine, Hefei, China.
FAU - Liu, Pei-Pei
AU  - Liu PP
AD  - Department of Integrated Traditional Chinese and Western Medicine, Anhui 
      University of Chinese Medicine, Hefei, China.
FAU - Bao, Guan-Hu
AU  - Bao GH
AUID- ORCID: 0000-0003-4336-5678
AD  - Natural Products Laboratory, State Key Laboratory of Tea Plant Biology and 
      Utilization, Anhui Agricultural University, Hefei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210526
PL  - United States
TA  - J Food Biochem
JT  - Journal of food biochemistry
JID - 7706045
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Kaempferols)
RN  - 0 (Tea)
RN  - 4056D20K3H (kaempferol-3-O-rutinoside)
SB  - IM
MH  - Animals
MH  - *Anti-Inflammatory Agents/pharmacology
MH  - China
MH  - Kaempferols
MH  - Molecular Docking Simulation
MH  - Protein Binding
MH  - Rats
MH  - *Tea
OTO - NOTNLM
OT  - Lu'an GuaPian tea
OT  - TLR4/MyD88/NF-kappaB signaling
OT  - anti-inflammatory effects
OT  - kaempferol-3-O-rutinoside
OT  - plasma protein binding
EDAT- 2021/05/28 06:00
MHDA- 2021/07/23 06:00
CRDT- 2021/05/27 07:06
PHST- 2021/04/13 00:00 [revised]
PHST- 2021/02/05 00:00 [received]
PHST- 2021/04/15 00:00 [accepted]
PHST- 2021/05/28 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2021/05/27 07:06 [entrez]
AID - 10.1111/jfbc.13749 [doi]
PST - ppublish
SO  - J Food Biochem. 2021 Jul;45(7):e13749. doi: 10.1111/jfbc.13749. Epub 2021 May 26.