PMID- 34043470
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20210812
IS  - 1522-1601 (Electronic)
IS  - 0161-7567 (Linking)
VI  - 131
IP  - 1
DP  - 2021 Jul 1
TI  - Effects of short-term graded dietary carbohydrate intake on intramuscular and 
      whole body metabolism during moderate-intensity exercise.
PG  - 376-387
LID - 10.1152/japplphysiol.00811.2020 [doi]
AB  - Altering dietary carbohydrate (CHO) intake modulates fuel utilization during 
      exercise. However, there has been no systematic evaluation of metabolic responses 
      to graded changes in short-term (< 1 wk) dietary CHO intake. Thirteen active men 
      performed interval running exercise combined with isocaloric diets over 3 days 
      before evaluation of metabolic responses to 60-min running at 65% V̇O(2max) on 
      three occasions. Diets contained lower [LOW, 2.40 +/- 0.66 g CHO.kg(-1).day(-1), 
      21.3 +/- 0.5% of energy intake (EI)], moderate (MOD, 4.98 +/- 1.31 g 
      CHO.kg(-1).day(-1), 46.3 +/- 0.7% EI), or higher (HIGH, 6.48 +/- 1.56 g 
      CHO.kg(-1).day(-1), 60.5 +/- 1.6% EI) CHO. Preexercise muscle glycogen content was 
      lower in LOW [54.3 +/- 26.4 mmol.kg(-1) wet weight (ww)] compared with MOD (82.6 +/- 
      18.8 mmol.kg( -1) ww) and HIGH (80.4 +/- 26.0 mmol.kg(-1) ww, P < 0.001; MOD vs. 
      HIGH, P = 0.85). Whole body substrate oxidation, systemic responses, and muscle 
      substrate utilization during exercise indicated increased fat and decreased CHO 
      metabolism in LOW [respiratory exchange ratio (RER): 0.81 +/- 0.01] compared with 
      MOD (RER 0.86 +/- 0.01, P = 0.0005) and HIGH (RER: 0.88 +/- 0.01, P < 0.0001; MOD vs. 
      HIGH, P = 0.14). Higher basal muscle expression of genes encoding proteins 
      implicated in fat utilization was observed in LOW. In conclusion, muscle glycogen 
      availability and subsequent metabolic responses to exercise were resistant to 
      increases in dietary CHO intake from  approximately 5.0 to  approximately 6.5 g CHO.kg(-1).day(-1) (46% to 
      61% EI), while muscle glycogen, gene expression, and metabolic responses were 
      sensitive to more marked reductions in CHO intake ( approximately 2.4 g CHO.kg(-1).day(-1), 
       approximately 21% EI).NEW & NOTEWORTHY The data presented here suggest that metabolic 
      responses to steady-state aerobic exercise are somewhat resistant to short-term 
      changes in dietary carbohydrate (CHO) intake within the 5-6.5 g 
      CHO.kg(-1).day(-1) [46-61% energy intake (EI)] range. In contrast, reduction in 
      short-term dietary CHO intake to  approximately 2.4 g CHO.kg(-1).day(-1) (21% EI) evoked clear 
      changes indicative of increased fat and decreased CHO metabolism during exercise.
FAU - Maunder, Ed
AU  - Maunder E
AD  - Sports Performance Research Institute New Zealand, Auckland University of 
      Technology, Auckland, New Zealand.
FAU - Bradley, Helen E
AU  - Bradley HE
AD  - School of Sport, Exercise, and Rehabilitation Sciences, University of Birmingham, 
      Birmingham, United Kingdom.
FAU - Deane, Colleen S
AU  - Deane CS
AUID- ORCID: 0000-0002-2281-6479
AD  - Department of Sport and Health Sciences, College of Life and Environmental 
      Sciences, University of Exeter, Exeter, United Kingdom.
AD  - Living Systems Institute, University of Exeter, Exeter, United Kingdom.
FAU - Hodgson, Adrian B
AU  - Hodgson AB
AD  - Lucozade Ribena Suntory Limited, Uxbridge, United Kingdom.
FAU - Jones, Michael
AU  - Jones M
AD  - School of Sport, Exercise, and Rehabilitation Sciences, University of Birmingham, 
      Birmingham, United Kingdom.
FAU - Joanisse, Sophie
AU  - Joanisse S
AD  - Department of Sport and Exercise Sciences, Manchester Metropolitan University, 
      Manchester, United Kingdom.
FAU - Turner, Alice M
AU  - Turner AM
AD  - Institute for Applied Health Research, College of Medical and Dental Sciences, 
      University of Birmingham, Birmingham, United Kingdom.
AD  - University Hospitals Birmingham National Health Services Foundation Trust, 
      Heartlands Hospital, Birmingham, United Kingdom.
FAU - Breen, Leigh
AU  - Breen L
AD  - School of Sport, Exercise, and Rehabilitation Sciences, University of Birmingham, 
      Birmingham, United Kingdom.
FAU - Philp, Andrew
AU  - Philp A
AD  - Healthy Ageing Research Theme, Garvan Institute of Medical Research, 
      Darlinghurst, New South Wales, Australia.
AD  - St. Vincent's Medical School, University of New South Wales Medicine, University 
      of New South Wales Sydney, Sydney, New South Wales, Australia.
FAU - Wallis, Gareth A
AU  - Wallis GA
AUID- ORCID: 0000-0002-1400-0859
AD  - School of Sport, Exercise, and Rehabilitation Sciences, University of Birmingham, 
      Birmingham, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210527
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Dietary Carbohydrates)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Carbohydrate Metabolism
MH  - Dietary Carbohydrates/metabolism
MH  - Exercise
MH  - Glycogen/metabolism
MH  - Humans
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Oxygen Consumption
MH  - *Physical Endurance
MH  - *Running
OTO - NOTNLM
OT  - diet
OT  - exercise
OT  - fat metabolism
OT  - muscle glycogen
OT  - running economy
EDAT- 2021/05/28 06:00
MHDA- 2021/07/23 06:00
CRDT- 2021/05/27 17:21
PHST- 2021/05/28 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2021/05/27 17:21 [entrez]
AID - 10.1152/japplphysiol.00811.2020 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2021 Jul 1;131(1):376-387. doi: 
      10.1152/japplphysiol.00811.2020. Epub 2021 May 27.