PMID- 34044335
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 171
DP  - 2021 Sep 1
TI  - Effect of kisspeptin (KISS) and RFamide-related peptide-3 (RFRP-3) on the 
      synthesis and secretion of FSH in vitro by pituitary cells in pigs.
PG  - 72-84
LID - S0093-691X(21)00170-9 [pii]
LID - 10.1016/j.theriogenology.2021.05.010 [doi]
AB  - Kisspeptins (KISSs) and RFamide-related peptide-3 (RFRP-3) affect the synthesis 
      and secretion of luteinizing hormone (LH) and modulate female reproductive 
      processes. The presence of KISS and RFRP-3 in the porcine pituitary gland and 
      their contribution to the regulation of follicle-stimulating hormone (FSH) 
      synthesis and secretion is unknown. This study analyzed the presence of KISS and 
      RFRP-3 in the pituitary of estrous-cyclic pigs on days 2 to 3, 10 to 11, 12 to 
      13, 15 to 16 and 19 to 20 and early pregnant pigs on days 10 to 11, 12 to 13 and 
      15 to 16, and evaluated the effect of KISS and RFRP-3 on beta-Fsh mRNA expression 
      and FSH secretion in vitro by pituitary cells collected on selected days of the 
      estrous cycle. The cells were cultured in vitro and treated with KISS (10(-6) M, 
      10(-7) M) and RFRP-3 (10(-6) M, 10(-7) M) or gonadotropin-releasing hormone 
      (GnRH; 100 ng/mL) alone and in combinations (4 h or 24 h). The relative abundance 
      of Kiss and Rfrp-3 and their receptor mRNA transcripts, as well as the KISS and 
      RFRP-3 proteins, were found in the pituitaries of estrous-cyclic and early 
      pregnant pigs. KISS after 4 h increased the secretion of FSH in estrous cyclic 
      pigs mostly during the early-luteal phase and luteolysis. RFRP-3 inhibited the 
      synthesis and secretion of FSH in estrous-cyclic pigs on days 19 to 20 and the 
      secretion of FSH on days 2 to 3 and 10 to 12 of the estrous cycle compared with 
      GnRH-treated cells. KISS in co-treatment with GnRH after 24 h enhanced FSH 
      release on days 2 to 3 and 15 to 16 of the estrous cycle. In conclusion, KISS and 
      RFRP-3 systems are present in the pituitary of estrous-cyclic and pregnant pigs. 
      In estrous-cyclic pigs, KISS and RFRP-3 may affect the synthesis and secretion of 
      FSH by pituitary cells.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Zmijewska, Agata
AU  - Zmijewska A
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-719, 
      Olsztyn, Poland. Electronic address: agata.zmijewska@uwm.edu.pl.
FAU - Czelejewska, Wioleta
AU  - Czelejewska W
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-719, 
      Olsztyn, Poland.
FAU - Drzewiecka, Ewa M
AU  - Drzewiecka EM
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-719, 
      Olsztyn, Poland.
FAU - Franczak, Anita
AU  - Franczak A
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-719, 
      Olsztyn, Poland.
LA  - eng
PT  - Journal Article
DEP - 20210513
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Kisspeptins)
RN  - 0 (Neuropeptides)
RN  - 0 (RFamide peptide)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Female
MH  - *Follicle Stimulating Hormone
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Kisspeptins/genetics
MH  - *Neuropeptides/genetics
MH  - Pituitary Gland/metabolism
MH  - Swine
OTO - NOTNLM
OT  - FSH
OT  - Kisspeptin
OT  - Pig
OT  - Pituitary
OT  - RFRP-3
COIS- Declaration of competing interest None.
EDAT- 2021/05/28 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/05/27 20:36
PHST- 2020/09/07 00:00 [received]
PHST- 2021/04/17 00:00 [revised]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/05/28 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/05/27 20:36 [entrez]
AID - S0093-691X(21)00170-9 [pii]
AID - 10.1016/j.theriogenology.2021.05.010 [doi]
PST - ppublish
SO  - Theriogenology. 2021 Sep 1;171:72-84. doi: 10.1016/j.theriogenology.2021.05.010. 
      Epub 2021 May 13.