PMID- 34047100
OWN - NLM
STAT- MEDLINE
DCOM- 20210531
LR  - 20210531
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 46
IP  - 10
DP  - 2021 May
TI  - [Simultaneous determination of multiple bioactive constituents in Abelmoschi 
      Corolla by UFLC-QTRAP-MS/MS].
PG  - 2527-2536
LID - 10.19540/j.cnki.cjcmm.20201225.302 [doi]
AB  - A comprehensive analytical method based on ultra-fast liquid chromatography 
      coupled with triple quadrupole/linear ion trap tandem mass 
      spectrometry(UFLC-QTRAP-MS/MS) was established for simultaneous determination of 
      the content of 38 active components in Abelmoschi Corolla, including flavonoids, 
      organic acids, nucleosides and amino acids, so as to investigate the effects of 
      different harvesting and processing methods on multi-active components in 
      Abelmoschi Corolla. The chromatographic separation was performed on a 
      XBridg(R)C_(18) column(4.6 mmx100 mm, 3.5 mum) with(0.1% formic acid water) 
      methanol-acetonitrile(1ratio1) as the mobile phase for gradient elution at 30 ℃. The 
      flow rate was 0.5 mL.min~(-1). The components were detected in a 
      multiple-reaction monitoring(MRM) mode. The gray relational analysis(GRA) was 
      used to comprehensively evaluate the multiple active components of Abelmoschi 
      Corolla at different harvesting times and drying temperatures. The results showed 
      that 38 components had a good linearity with correlation coefficients all above 
      0.999 0. The method featured a good precision, repeatability and stability with 
      the relative stan-dard deviations(RSDs) of less than 5.0%. Recoveries ranged from 
      98.06% to 104.4% with RSD between 0.22% and 4.9%. The results of GRA indicated 
      that a better quality in the samples collected on September 9 th. Samples dried 
      at 90 ℃ had a better quality. The established method is accurate and reliable, 
      and can be used to assess the internal quality of Abelmoschi Corolla. This study 
      can provide basic materials for determining appropriate harvesting time and 
      processing method of Abelmoschi Corolla.
FAU - Yin, Sheng-Xin
AU  - Yin SX
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Wei, Li-Fang
AU  - Wei LF
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Mei, Yu-Qi
AU  - Mei YQ
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Liu, Xun-Hong
AU  - Liu XH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Zou, Li-Si
AU  - Zou LS
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Cai, Zhi-Chen
AU  - Cai ZC
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yuan, Jia-Huan
AU  - Yuan JH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Ge, Hai-Tao
AU  - Ge HT
AD  - Jiangsu Suzhong Pharmaceutical Group Co., Ltd. Taizhou 225500, China.
FAU - Wang, Dian-Guang
AU  - Wang DG
AD  - Jiangsu Suzhong Pharmaceutical Group Co., Ltd. Taizhou 225500, China.
FAU - Wang, Dan-Dan
AU  - Wang DD
AD  - Jiangsu Suzhong Pharmaceutical Group Co., Ltd. Taizhou 225500, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Amino Acids)
RN  - 0 (Nucleosides)
SB  - IM
MH  - Amino Acids
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - *Nucleosides
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Abelmoschi Corolla
OT  - UFLC-QTRAP-MS/MS
OT  - bioactive constituents
OT  - gray relational analysis
OT  - harvesting and processing
EDAT- 2021/05/29 06:00
MHDA- 2021/06/01 06:00
CRDT- 2021/05/28 07:55
PHST- 2021/05/28 07:55 [entrez]
PHST- 2021/05/29 06:00 [pubmed]
PHST- 2021/06/01 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20201225.302 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2021 May;46(10):2527-2536. doi: 
      10.19540/j.cnki.cjcmm.20201225.302.