PMID- 34047671
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20220204
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Dec
TI  - Human umbilical cord-derived mesenchymal stem cells improve chronic pancreatitis 
      in rats via the AKT-mTOR-S6K1 signaling pathway.
PG  - 1986-1996
LID - 10.1080/21655979.2021.1928441 [doi]
AB  - Chronic pancreatitis (CP) is a progressive inflammatory disease. In clinical 
      treatment, many patients cannot get a timely diagnosis and effective treatment 
      due to the lack of early diagnosis indicators. Mesenchymal stem cells have 
      immunomodulatory and anti-inflammatory effects, and have broad application 
      prospects in treating auto-immune diseases and inflammatory diseases. This study 
      aimed to clarify the mechanisms of human umbilical cord mesenchymal stem cells 
      (HUCMSCs) in the treatment of CP. The rats were randomly divided into four 
      groups, with six rats in each group: control group, CP group, CP + 
      HUCMSCs-treated group I, and CP + HUCMSCs-treated group II. We evaluated the 
      levels of inflammatory factors, fibrosis and apoptosis markers, detected the 
      protein expression levels of AKT-mTOR-S6K1 and assessed histological changes of 
      the pancreas. The results showed that HUCMSCs not only inhibited the secretion of 
      inflammatory cytokines and activation of pancreatic stellate cells but also 
      suppressed the apoptosis of acinar cells. Further investigation revealed that 
      HUCMSCs noticeably suppressed the AKT-mTOR-S6K1 pathway in the pancreatic tissue 
      of DBTC-induced CP. In addition, the therapeutic effect of HUCMSCs injected into 
      the inferior vena cava and left gastric artery in the CP model was also observed, 
      thus providing the basis for the clinical application of intervention measures.
FAU - Kong, Lijun
AU  - Kong L
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Xu, Xiangxiang
AU  - Xu X
AD  - Ophthalmology Department, The Yiling Hospital of Yichang, Yichang, Hubei, China.
FAU - Zhang, Hewei
AU  - Zhang H
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Huang, Hongjian
AU  - Huang H
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Chen, Bicheng
AU  - Chen B
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Zhou, Zhenxu
AU  - Zhou Z
AUID- ORCID: 0000-0002-9294-6332
AD  - Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (Cytokines)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (Rps6kb1 protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fibrosis/metabolism
MH  - Human Umbilical Vein Endothelial Cells/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Pancreas/cytology/metabolism
MH  - Pancreatic Stellate Cells/metabolism
MH  - Pancreatitis, Chronic/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Rats
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - Signal Transduction/physiology
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC8806739
OTO - NOTNLM
OT  - Chronic pancreatitis
OT  - fibrosis
OT  - inflammation
OT  - mesenchymal stem cell
OT  - pancreatic stellate cells
EDAT- 2021/05/29 06:00
MHDA- 2021/12/01 06:00
PMCR- 2021/05/28
CRDT- 2021/05/28 12:11
PHST- 2021/05/28 12:11 [entrez]
PHST- 2021/05/29 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2021/05/28 00:00 [pmc-release]
AID - 1928441 [pii]
AID - 10.1080/21655979.2021.1928441 [doi]
PST - ppublish
SO  - Bioengineered. 2021 Dec;12(1):1986-1996. doi: 10.1080/21655979.2021.1928441.