PMID- 34048096
OWN - NLM
STAT- MEDLINE
DCOM- 20211221
LR  - 20240402
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 35
IP  - 7
DP  - 2021 Jul
TI  - tRNA(Ini) (CAT) inhibits proliferation and promotes apoptosis of laryngeal 
      squamous cell carcinoma cells.
PG  - e23821
LID - 10.1002/jcla.23821 [doi]
LID - e23821
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) brings a heavy blow to the 
      patient's voice. Transfer RNA (tRNA) is a common RNA, the roles of tRNAs in LSCC 
      are largely unknown. METHODS: The tRNA expression profile in LSCC tissues and 
      adjacent normal tissues was measured by a tRNA qRT-PCR array. The expression 
      level of tRNA(Ini) (CAT) in LSCC tissues and plasmas was detected by qRT-PCR. The 
      receiver operating characteristic (ROC) curve was established. tRNA(Ini) (CAT) 
      was upregulated by a lentivirus vector in the LSCC cell line. Moreover, tRNA(Ini) 
      (CAT) was upregulated in LSCC xenograft nude mouse model and the xenografts were 
      used for pathological analysis and transmission electron microscope (TEM) 
      observation. RESULTS: The top 10 upregulated tRNAs were tRNA(Lys) (CTT) -1, 
      tRNA(Leu) (TAA) , tRNA(Phe) (GAA) , tRNA(Leu) (CAG) , tRNA(Tyr) (ATA) , tRNA(Met) 
      (CAT) , tRNA(Tyr) (GTA) -1, tRNA(Thr) (CGT) , tRNA(Tyr) (GTA) -2, tRNA(Ala) (AGC) 
      ; and the top 10 downregulated tRNAs were tRNA(Ini) (CAT) , mt-tRNA(Glu) (TTC) , 
      tRNA(Val) (CAC) -3, mt-tRNA(Trp) (TCA) , mt-tRNA(Tyr) (GTA) , mt-tRNA(Lys) (TTT) 
      , mt-tRNA(Thr) (TGT) , mt-tRNA(Asp) (GTC) , mt-tRNA(Asn) (GTT) , mt-tRNA(Pro) 
      (TGG) . tRNA(Ini) (CAT) was downregulated in LSCC tissues and plasma. The area 
      under the ROC curve (AUC) in LSCC tissues and the plasma of patients with LSCC 
      was 0.717 and 0.808, respectively. tRNA(Ini) (CAT) inhibited LSCC cell 
      proliferation and promoted apoptosis. The in vivo results showed that tRNA(Ini) 
      (CAT) inhibited the growth of the xenografts and promoted apoptosis. CONCLUSIONS: 
      This is the first study to provide tRNA expression profiles for LSCC tissues. 
      tRNA(Ini) (CAT) may be used as a new biomarker for the early diagnosis of LSCC. 
      tRNA(Ini) (CAT) inhibits cell proliferation and promotes apoptosis in vitro and 
      in vivo.
CI  - (c) 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Li, Jun
AU  - Li J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
AD  - Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of 
      Pathophysiology, Ningbo University School of Medical, Ningbo, China.
FAU - Shen, Zhisen
AU  - Shen Z
AUID- ORCID: 0000-0001-9958-3100
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
FAU - Luo, Lin
AU  - Luo L
AD  - Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of 
      Pathophysiology, Ningbo University School of Medical, Ningbo, China.
FAU - Ye, Dong
AU  - Ye D
AUID- ORCID: 0000-0003-2055-0256
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
FAU - Gu, Shanshan
AU  - Gu S
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
FAU - Zhou, Chongchang
AU  - Zhou C
AUID- ORCID: 0000-0002-8728-6819
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated LiHuili 
      Hospital, Ningbo University, Ningbo, China.
LA  - eng
GR  - PPXK2018-02/Ningbo Health Branding Subject Fund/
GR  - LQ21H130001/Natural Science Foundation of Zhejiang Province/
GR  - LY19H160014/Natural Science Foundation of Zhejiang Province/
GR  - LY20H130001/Natural Science Foundation of Zhejiang Province/
GR  - 2018B10015/Ningbo "Technology Innovation 2025" Major Special Project/
GR  - 2020Z097/Ningbo "Technology Innovation 2025" Major Special Project/
GR  - 2018A610361/Natural Science Foundation of Ningbo/
GR  - 2019A610319/Natural Science Foundation of Ningbo/
GR  - 202003N4239/Natural Science Foundation of Ningbo/
GR  - 2019ZD018/Medical and Health Research Project of Zhejiang Province/
GR  - 2021KY307/Medical and Health Research Project of Zhejiang Province/
PT  - Journal Article
DEP - 20210528
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Carcinoma, Squamous Cell/blood/diagnosis/*genetics/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/blood/diagnosis/*genetics/*pathology
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - RNA, Transfer/blood/genetics/*metabolism
MH  - ROC Curve
MH  - Up-Regulation/genetics
MH  - Xenograft Model Antitumor Assays
MH  - Mice
PMC - PMC8274982
OTO - NOTNLM
OT  - biomarkers
OT  - laryngeal squamous cell carcinoma
OT  - tRNA
OT  - tRNA expression profile
OT  - tRNAIniCAT
COIS- The authors declare no conflict of interest.
EDAT- 2021/05/29 06:00
MHDA- 2021/12/22 06:00
PMCR- 2021/05/28
CRDT- 2021/05/28 12:27
PHST- 2021/04/21 00:00 [revised]
PHST- 2021/03/22 00:00 [received]
PHST- 2021/04/22 00:00 [accepted]
PHST- 2021/05/29 06:00 [pubmed]
PHST- 2021/12/22 06:00 [medline]
PHST- 2021/05/28 12:27 [entrez]
PHST- 2021/05/28 00:00 [pmc-release]
AID - JCLA23821 [pii]
AID - 10.1002/jcla.23821 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2021 Jul;35(7):e23821. doi: 10.1002/jcla.23821. Epub 2021 May 
      28.