PMID- 34050658
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220524
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 46
IP  - 5
DP  - 2022 May 20
TI  - Determination of Drugs of Abuse in Hair by LC-MS-MS: Application to Suicide 
      Attempts Investigation.
PG  - 577-581
LID - 10.1093/jat/bkab058 [doi]
AB  - Considering that the use of psychoactive substances (PSs) is a risk factor to 
      either higher intensity or frequency of suicidal behavior, hair analysis was 
      conducted to investigate the most consumed PSs (opiates, amphetamine stimulants, 
      marijuana, cocaine and heroin) in patients who attempted suicide and received 
      urgent care at emergency service. Hair samples were extracted using methanol and 
      sonicated under heating and then analyzed using liquid chromatography-tandem mass 
      spectrometry. During validation, the method complied with international 
      recommended criteria, with limits of detection between 0.0025 and 0.05 ng/mg and 
      linearity between 0.1 and 4 ng/mg for methamphetamine, 
      3,4-methylenedioxymethamphetamine (MDMA), morphine, amphetamine, 
      6-acetylmorphine, 3,4-methylenedioxyamphetamine (MDA), fenproporex, 
      diethylpropion and codeine; between 0.025 and 1 ng/mg for tetrahydrocannabinol 
      (THC), benzoylecgonine and cocaethylene and between 0.25 and 10 ng/mg for cocaine 
      and mazindol. A total of 109 hair samples were analyzed and segmented in 404 
      parts. Among all analyzed samples, 30.3% were positive for at least one PS 
      (n = 33), such as cocaine (90.9%), codeine (12.1%), morphine (3.0%), MDMA (3.0%) 
      and THC (3.0%). In segmental analysis of cocaine positive samples (n = 30), 76.7% 
      of the samples indicated recent exposure to cocaine (<1 month). This same 
      behavior was observed when analyzing codeine (n = 4) and morphine (n = 1). THC 
      positive samples indicated exposure dated  approximately 4 months prior. In conclusion, the 
      method was validated following international recommendations for the 12 most 
      consumed PSs in Brazil, as well as two of the most common found metabolites.
CI  - (c) The Author(s) 2021. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Cardoso, Marilia S
AU  - Cardoso MS
AD  - Faculty of Medical Sciences, Universidade Estadual de Campinas, Tessalia Vieira 
      de Camargo, 126 Cidade Universitaria, Campinas, Sao Paulo 13083-887, Brazil.
AD  - Campinas Poison, Control Center, University of Campinas, R. Vital Brasil, 251 
      Barao Geraldo, Campinas, Sao Paulo 13083-881, Brazil.
FAU - Lanaro, Rafael
AU  - Lanaro R
AD  - Faculty of Medical Sciences, Universidade Estadual de Campinas, Tessalia Vieira 
      de Camargo, 126 Cidade Universitaria, Campinas, Sao Paulo 13083-887, Brazil.
AD  - Campinas Poison, Control Center, University of Campinas, R. Vital Brasil, 251 
      Barao Geraldo, Campinas, Sao Paulo 13083-881, Brazil.
FAU - Dolores, Raul C
AU  - Dolores RC
AD  - Faculty of Medical Sciences, Universidade Estadual de Campinas, Tessalia Vieira 
      de Camargo, 126 Cidade Universitaria, Campinas, Sao Paulo 13083-887, Brazil.
AD  - R. Mario Giordano, 146 Antiga 33, Jardim America, Paulinia, Sao Paulo 13140-614, 
      Brazil.
FAU - Morais, Damila R
AU  - Morais DR
AD  - Shimadzu Brazil, Av. Tambore, 576 Tambore, Barueri, Sao Paulo 06460-000, Brazil.
FAU - Arantes, Ana Carolina Furiozo
AU  - Arantes ACF
AD  - Faculty of Medical Sciences, Universidade Estadual de Campinas, Tessalia Vieira 
      de Camargo, 126 Cidade Universitaria, Campinas, Sao Paulo 13083-887, Brazil.
AD  - Campinas Poison, Control Center, University of Campinas, R. Vital Brasil, 251 
      Barao Geraldo, Campinas, Sao Paulo 13083-881, Brazil.
FAU - Oliveira, Karina Diniz
AU  - Oliveira KD
AD  - Faculty of Medical Sciences, Universidade Estadual de Campinas, Tessalia Vieira 
      de Camargo, 126 Cidade Universitaria, Campinas, Sao Paulo 13083-887, Brazil.
FAU - Costa, Jose Luiz
AU  - Costa JL
AUID- ORCID: 0000-0001-9607-3391
AD  - Campinas Poison, Control Center, University of Campinas, R. Vital Brasil, 251 
      Barao Geraldo, Campinas, Sao Paulo 13083-881, Brazil.
AD  - Faculty of Pharmaceutical Sciences, University of Campinas, R. Candido Portinari, 
      200 Cidade Universitaria, Campinas, Sao Paulo 13083-871, Brazil.
LA  - eng
GR  - 2017/00274-4/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 2018/00432-1/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - Finance Code 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Morphine Derivatives)
RN  - 76I7G6D29C (Morphine)
RN  - 7J8897W37S (Dronabinol)
RN  - I5Y540LHVR (Cocaine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - UX6OWY2V7J (Codeine)
SB  - IM
MH  - Amphetamines/analysis
MH  - Chromatography, Liquid/methods
MH  - *Cocaine/analysis
MH  - Codeine/analysis
MH  - Dronabinol/analysis
MH  - Hair/chemistry
MH  - Humans
MH  - Morphine/analysis
MH  - Morphine Derivatives/analysis
MH  - *N-Methyl-3,4-methylenedioxyamphetamine/analysis
MH  - Substance Abuse Detection/methods
MH  - Suicide, Attempted
MH  - Tandem Mass Spectrometry/methods
EDAT- 2021/05/30 06:00
MHDA- 2022/05/25 06:00
CRDT- 2021/05/29 08:33
PHST- 2020/12/15 00:00 [received]
PHST- 2021/04/12 00:00 [revised]
PHST- 2021/05/28 00:00 [accepted]
PHST- 2021/05/30 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2021/05/29 08:33 [entrez]
AID - 6288373 [pii]
AID - 10.1093/jat/bkab058 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2022 May 20;46(5):577-581. doi: 10.1093/jat/bkab058.