PMID- 34052691 OWN - NLM STAT- MEDLINE DCOM- 20210921 LR - 20210921 IS - 1879-0046 (Electronic) IS - 0376-8716 (Linking) VI - 225 DP - 2021 Aug 1 TI - Effect of oral alcohol administration on plasma cytokine concentrations in heavy drinking individuals. PG - 108771 LID - S0376-8716(21)00266-0 [pii] LID - 10.1016/j.drugalcdep.2021.108771 [doi] AB - BACKGROUND: Alcohol is known to modulate the immune system, including cytokines, under conditions of both acute consumption and chronic use. The specific pro- and anti-inflammatory effects and mechanisms whereby alcohol consumption modulates circulating cytokine concentrations are not well understood. Few studies in humans have investigated the effect of acute alcohol consumption on plasma cytokine concentrations in individuals who are heavy drinkers. METHODS: Data were pooled from two studies involving a total of 25 non-treatment seeking, heavy drinking individuals who undertook an oral alcohol administration procedure. Plasma cytokine [Interleukin-10 (IL-10), Interleukin-6 (IL-6), Interleukin-18 (IL-18) and Tumor Necrosis Factor-alpha (TNF-alpha)] concentrations were measured at two baseline timepoints, then three hours after alcohol administration, and finally when breath alcohol concentrations returned to zero. Linear mixed models were conducted to determine whether there was a significant effect of time on cytokine concentrations. RESULTS: There was a significant reduction in TNF-alpha concentration (F [3, 20.42] = 4.96, p = 0.01, eta(2)(p) = 0.42) post alcohol administration, compared to baseline concentrations, and a significant increase in IL-6 concentrations (F [3, 27.81] = 9.06, p < 0.001, eta(2)(p) = 0.49) post alcohol administration, compared to baseline. There were no significant changes in IL-18 or IL-10 concentrations. CONCLUSIONS: To our knowledge, this is the first study to examine the acute effect of oral alcohol consumption on peripheral inflammatory markers in individuals with alcohol use disorder. Results indicate a clinically relevant increase in proinflammatory cytokines approximately 3 h after initial alcohol ingestion. Further research should be done to elucidate the complex interaction between alcohol and the immune system. CI - Published by Elsevier B.V. FAU - Lee, Mary R AU - Lee MR AD - Clinical Psychoneuroendocrinology and Neuropsychopharmacology Section, Translational Addiction Medicine Branch, National Institute on Drug Abuse Intramural Research Program and National Institute on Alcohol Abuse and Alcoholism Division of Intramural Clinical and Biological Research, National Institutes of Health, Baltimore and Bethesda, MD, USA. Electronic address: leemary@mail.nih.gov. FAU - Abshire, Kelly M AU - Abshire KM AD - Clinical Psychoneuroendocrinology and Neuropsychopharmacology Section, Translational Addiction Medicine Branch, National Institute on Drug Abuse Intramural Research Program and National Institute on Alcohol Abuse and Alcoholism Division of Intramural Clinical and Biological Research, National Institutes of Health, Baltimore and Bethesda, MD, USA. FAU - Farokhnia, Mehdi AU - Farokhnia M AD - Clinical Psychoneuroendocrinology and Neuropsychopharmacology Section, Translational Addiction Medicine Branch, National Institute on Drug Abuse Intramural Research Program and National Institute on Alcohol Abuse and Alcoholism Division of Intramural Clinical and Biological Research, National Institutes of Health, Baltimore and Bethesda, MD, USA; Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA. FAU - Akhlaghi, Fatemeh AU - Akhlaghi F AD - Clinical Pharmacokinetics Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island Kingston, RI, USA. FAU - Leggio, Lorenzo AU - Leggio L AD - Clinical Psychoneuroendocrinology and Neuropsychopharmacology Section, Translational Addiction Medicine Branch, National Institute on Drug Abuse Intramural Research Program and National Institute on Alcohol Abuse and Alcoholism Division of Intramural Clinical and Biological Research, National Institutes of Health, Baltimore and Bethesda, MD, USA; Medication Development Program, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD, USA; Center for Alcohol and Addiction Studies, Department of Behavioral and Social Sciences, School of Public Health, Brown University, Providence, RI, USA; Division of Addiction Medicine, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Neuroscience, Georgetown University Medical Center, Washington DC, USA. Electronic address: lorenzo.leggio@nih.gov. LA - eng GR - ZIA AA000218/ImNIH/Intramural NIH HHS/United States GR - ZIA DA000635/ImNIH/Intramural NIH HHS/United States GR - UH2 TR000963/TR/NCATS NIH HHS/United States GR - UH3 TR000963/TR/NCATS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't DEP - 20210521 PL - Ireland TA - Drug Alcohol Depend JT - Drug and alcohol dependence JID - 7513587 RN - 0 (Cytokines) RN - 0 (Tumor Necrosis Factor-alpha) RN - 3K9958V90M (Ethanol) SB - IM MH - Alcohol Drinking MH - *Alcoholism MH - *Cytokines MH - Ethanol MH - Humans MH - Tumor Necrosis Factor-alpha OTO - NOTNLM OT - Alcohol OT - Cytokines OT - IL-10 OT - IL-18 OT - IL-6 OT - Inflammation OT - TNF-alpha EDAT- 2021/05/31 06:00 MHDA- 2021/09/22 06:00 CRDT- 2021/05/30 20:57 PHST- 2021/02/17 00:00 [received] PHST- 2021/04/13 00:00 [revised] PHST- 2021/04/17 00:00 [accepted] PHST- 2021/05/31 06:00 [pubmed] PHST- 2021/09/22 06:00 [medline] PHST- 2021/05/30 20:57 [entrez] AID - S0376-8716(21)00266-0 [pii] AID - 10.1016/j.drugalcdep.2021.108771 [doi] PST - ppublish SO - Drug Alcohol Depend. 2021 Aug 1;225:108771. doi: 10.1016/j.drugalcdep.2021.108771. Epub 2021 May 21.