PMID- 34053439
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 21
IP  - 1
DP  - 2021 May 30
TI  - Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen 
      peroxide via the sarA.
PG  - 160
LID - 10.1186/s12866-021-02218-x [doi]
LID - 160
AB  - BACKGROUND: The infectious pathogen Staphylococcus aureus (S. aureus) is 
      primarily associated with osteomyelitis. Hydrogen peroxide drainage is an 
      effective antimicrobial treatment that has been adopted to combat S. aureus 
      infections. Previous investigations have indicated that the antisense RNA (asRNA) 
      strategy negatively modulates S. aureus YycFG TCS, and it significantly disrupts 
      biofilm formation. However, the effects of the antisense yycG RNA (ASyycG) 
      strategy on the susceptibility of biofilm-producing S. aureus to hydrogen 
      peroxide and the mechanisms underlying this effect have not been elucidated to 
      date. RESULTS: Overexpression of ASyycG inhibited the transcription of biofilm 
      formation-related genes, including sarA and icaA. Additionally, the CFU counts 
      and the live bacterial ratios of ASyycG biofilm-producing S. aureus treated with 
      H(2)O(2) were notably reduced across the groups. Notably, the predicted promoter 
      regions of the sarA and icaA genes were directly regulated by YycF. CONCLUSIONS: 
      ASyycG was observed to sensitize biofilm-producing S. aureus to H(2)O(2) 
      intervention synergistically via the sarA and thus may represent a supplementary 
      strategy for managing osteomyelitis. However, future in-depth studies should 
      attempt to replicate our findings in animal models, such as the rat osteomyelitis 
      model.
FAU - Wu, Shizhou
AU  - Wu S
AD  - Department of Orthopedics, West China Hospital, Sichuan University, No.37 Guoxue 
      Alley, Sichuan, 610041, Chengdu, P.R. China.
FAU - Liu, Yunjie
AU  - Liu Y
AD  - West China School of Public Health, Sichuan University, Chengdu, China.
FAU - Lei, Lei
AU  - Lei L
AD  - Department of Preventive Dentistry, Hospital of Stomatology, State Key Laboratory 
      of Oral Diseases,, Sichuan University, NO.14 Third Section, Renmin South Road, 
      Sichuan, 610041, Chengdu, P.R. China. leilei@scu.edu.cn.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Department of Orthopedics, West China Hospital, Sichuan University, No.37 Guoxue 
      Alley, Sichuan, 610041, Chengdu, P.R. China. caesarzh@163.com.
LA  - eng
GR  - 2021YJ0455/Department of Science and Technology of Sichuan Province/
GR  - 2018SZ0125/Department of Science and Technology of Sichuan Province/
GR  - 2020HXBH134/West China Hospital, Sichuan University/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210530
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Antisense)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Proteins/genetics/metabolism
MH  - Biofilms/drug effects
MH  - Gene Expression Regulation, Bacterial/drug effects
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - RNA, Antisense/*genetics/metabolism
MH  - Staphylococcal Infections/*microbiology
MH  - Staphylococcus aureus/*drug effects/*genetics/physiology
PMC - PMC8165985
OTO - NOTNLM
OT  - Antisense
OT  - Hydrogen peroxide
OT  - Staphylococcus aureus
OT  - YycFG
OT  - sarA
COIS- The authors have no conflicts of interest to disclose in relation to this 
      article.
EDAT- 2021/06/01 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/05/30
CRDT- 2021/05/31 05:42
PHST- 2020/10/21 00:00 [received]
PHST- 2021/05/05 00:00 [accepted]
PHST- 2021/05/31 05:42 [entrez]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/05/30 00:00 [pmc-release]
AID - 10.1186/s12866-021-02218-x [pii]
AID - 2218 [pii]
AID - 10.1186/s12866-021-02218-x [doi]
PST - epublish
SO  - BMC Microbiol. 2021 May 30;21(1):160. doi: 10.1186/s12866-021-02218-x.