PMID- 34056287
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210601
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 6
IP  - 17
DP  - 2021 May 4
TI  - Comprehensive Quality Assessment of Kaixin Powder by HPLC-DAD Quantification and 
      HPLC-QTOF-MS/MS Confirmation.
PG  - 11319-11326
LID - 10.1021/acsomega.1c00289 [doi]
AB  - Kaixin Powder (KXP) is a classic formula for treating morbid forgetfulness in 
      ancient China. To guarantee the efficacy and safety of KXP, a simple and accurate 
      HPLC-DAD method has been established and validated for the quantitative analysis 
      of seven bioactive compounds in KXP. Dehydrotumulosic acid (DTU) and 
      dehydrotrametenolic acid (DTR) were quantified in KXP for the first time. Good 
      chromatographic separation was conducted on a Kromasil 100-5 C(18) column (250 mm 
      x 4.6 mm, 5 mum) by gradient elution using mobile phases containing acetonitrile 
      and 0.1% formic acid aqueous solution at different detection wavelengths. The 
      calibration curves of each compound showed good linearity (r >/= 0.9990), and the 
      LOD and LOQ were in the ranges of 0.01-0.10 and 0.03-0.40 mug/mL, respectively. 
      The relative standard deviations (RSDs) of intra-day and inter-day precisions 
      were in the ranges of 0.45-1.74% and 0.56-2.32%, respectively. All recoveries 
      were in the range of 93.6-105.5% with an RSD no more than 2.77%. These 
      quantification results of seven compounds determined in the samples were further 
      confirmed by HPLC-QTOF-MS/MS. This study provides a useful and simple method for 
      analyzing the major bioactive compounds and improves the quality assessment 
      research of KXP.
CI  - (c) 2021 The Authors. Published by American Chemical Society.
FAU - Wang, Binbin
AU  - Wang B
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
FAU - Feng, Xiaoxiao
AU  - Feng X
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
FAU - Liu, Sihan
AU  - Liu S
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
FAU - Qiu, Feng
AU  - Qiu F
AUID- ORCID: 0000-0002-9945-0612
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
FAU - Lu, Xuran
AU  - Lu X
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
FAU - Li, Zhaoxia
AU  - Li Z
AUID- ORCID: 0000-0003-1786-2451
AD  - Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional 
      Chinese Medicine, Capital Medical University, Beijing 100069, China.
LA  - eng
PT  - Journal Article
DEP - 20210421
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC8153899
COIS- The authors declare no competing financial interest.
EDAT- 2021/06/01 06:00
MHDA- 2021/06/01 06:01
PMCR- 2021/04/21
CRDT- 2021/05/31 06:22
PHST- 2021/01/16 00:00 [received]
PHST- 2021/04/07 00:00 [accepted]
PHST- 2021/05/31 06:22 [entrez]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/06/01 06:01 [medline]
PHST- 2021/04/21 00:00 [pmc-release]
AID - 10.1021/acsomega.1c00289 [doi]
PST - epublish
SO  - ACS Omega. 2021 Apr 21;6(17):11319-11326. doi: 10.1021/acsomega.1c00289. 
      eCollection 2021 May 4.