PMID- 34056770
OWN - NLM
STAT- MEDLINE
DCOM- 20211129
LR  - 20211129
IS  - 1098-2280 (Electronic)
IS  - 0893-6692 (Linking)
VI  - 62
IP  - 5
DP  - 2021 Jun
TI  - Investigating aneuploidy-inducing effect of Nemacur, Rogor, and Dursban in human 
      peripheral blood lymphocyte cultures.
PG  - 328-334
LID - 10.1002/em.22445 [doi]
AB  - For many years, organophosphate (OP) pesticides have been considered an 
      attractive choice for pest control around the world. Excessive use of OPs is a 
      concerning issue for human health. Although the genotoxic effect of these 
      pesticides has been reported, studies that examined their aneuploidy-inducing 
      effect are limited or absent. Therefore, we sought to investigate the potential 
      of OP pesticides, which are extensively used in the Gaza Strip, to induce 
      aneuploidy in human peripheral blood lymphocyte (PBL) cultures. To achieve this 
      goal, we first assessed the cytotoxic effect of selected concentrations of 
      Nemacur (fenamiphos), Rogor (dimethoate), and Dursban (chlorpyrifos) on human PBL 
      cultures by the MTT assay. Then, fluorescence in situ hybridization (FISH) 
      technique was used to determine the frequency of induced aneuploidy (chromosome 
      loss or gain) in human PBL cultures treated with different concentrations of the 
      three types of OPs. We found that all the OPs treatments used did not show 
      appreciable cytotoxic effects. Increase in frequencies of aneuploidy, chromosome 
      loss, and chromosome gain were observed after each treatment as compared to the 
      results of their respective solvent control cultures, and that increase of 
      aneuploidy was significantly evident at 0.050 mg/ml of Nemacur. It was also 
      noticed that chromosome loss is more frequent than chromosome gain for each 
      concentration of the three types of OPs. While the aneuploidy induction effect of 
      the investigated OPs is not significant (except for the 0.050 mg/ml of Nemacur), 
      these pesticides should be examined further since many people are exposed to 
      them.
CI  - (c) 2021 Environmental Mutagen Society.
FAU - Abuwarda, Hadeer N
AU  - Abuwarda HN
AUID- ORCID: 0000-0001-7281-5784
AD  - Department of Medical Laboratory Sciences, IUG, Gaza, Palestine.
FAU - Alashi, Shadi F
AU  - Alashi SF
AD  - Department of Medical Laboratory Sciences, IUG, Gaza, Palestine.
FAU - Sharif, Fadel A
AU  - Sharif FA
AD  - Department of Medical Laboratory Sciences, IUG, Gaza, Palestine.
LA  - eng
PT  - Journal Article
DEP - 20210607
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Cholinesterase Inhibitors)
RN  - 0 (Insecticides)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Pesticides)
RN  - H4NO3L2HBE (fenamiphos)
RN  - JCS58I644W (Chlorpyrifos)
RN  - W6U08B045O (Dimethoate)
SB  - IM
MH  - *Aneuploidy
MH  - Chlorpyrifos/*adverse effects
MH  - Cholinesterase Inhibitors/adverse effects
MH  - Chromosome Aberrations/*chemically induced
MH  - DNA Damage
MH  - Dimethoate/*adverse effects
MH  - Humans
MH  - Insecticides/adverse effects
MH  - Leukocytes, Mononuclear/drug effects/*pathology
MH  - Lymphocytes/drug effects/*pathology
MH  - Organophosphorus Compounds/*adverse effects
MH  - Pesticides/adverse effects
OTO - NOTNLM
OT  - FISH
OT  - chromosome gain
OT  - chromosome loss
OT  - organophosphate
EDAT- 2021/06/01 06:00
MHDA- 2021/11/30 06:00
CRDT- 2021/05/31 06:35
PHST- 2021/05/07 00:00 [revised]
PHST- 2020/11/30 00:00 [received]
PHST- 2021/05/11 00:00 [accepted]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/11/30 06:00 [medline]
PHST- 2021/05/31 06:35 [entrez]
AID - 10.1002/em.22445 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2021 Jun;62(5):328-334. doi: 10.1002/em.22445. Epub 2021 Jun 
      7.