PMID- 34056880
OWN - NLM
STAT- MEDLINE
DCOM- 20210913
LR  - 20210913
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 22
IP  - 3
DP  - 2021 May
TI  - Role of IFNLR1 gene in PRRSV infection of PAM cells.
PG  - e39
LID - 10.4142/jvs.2021.22.e39 [doi]
LID - e39
AB  - BACKGROUND: Interferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor 
      that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs 
      (IFN-lambdas). IFN-lambdas act through the JAK-STAT signaling pathway to exert antiviral 
      effects related to preventing and curing an infection. Although the immune 
      function of IFN-lambdas in virus invasion has been described, the molecular mechanism 
      of IFNLR1 in that process is unclear. OBJECTIVES: The purpose of this study was 
      to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine 
      reproductive and respiratory syndrome virus (PRRSV). METHODS: The effects of 
      IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV 
      infection were investigated using interference and overexpression methods. 
      RESULTS: In this study, the expressions of the IFNLR1 gene in the liver, large 
      intestine, small intestine, kidney, and lung tissues of Dapulian pigs were 
      significantly higher than those in Landrace pigs. It was determined that porcine 
      IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed 
      that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. 
      IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of 
      p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. 
      The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the 
      opposite change was observed in IFNLR1-underexpressing cells. After inhibition of 
      the JAK/STAT signaling pathway, the G2/M phase proportion in the 
      IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, 
      overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby 
      inhibiting the proliferation of PAMs infected with PRRSV. CONCLUSION: Expression 
      of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, 
      indicating it has potential as a molecular target in developing a new strategy 
      for the treatment of PRRSV.
CI  - (c) 2021 The Korean Society of Veterinary Science.
FAU - Qin, Ming
AU  - Qin M
AUID- ORCID: 0000-0003-2212-2626
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Chen, Wei
AU  - Chen W
AUID- ORCID: 0000-0002-2901-5156
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Li, Zhixin
AU  - Li Z
AUID- ORCID: 0000-0002-0218-1680
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Wang, Lixue
AU  - Wang L
AUID- ORCID: 0000-0001-9704-1527
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Ma, Lixia
AU  - Ma L
AUID- ORCID: 0000-0001-9475-7650
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Geng, Jinhong
AU  - Geng J
AUID- ORCID: 0000-0002-5784-9238
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Zhang, Yu
AU  - Zhang Y
AUID- ORCID: 0000-0002-4326-6031
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Zhao, Jing
AU  - Zhao J
AUID- ORCID: 0000-0002-4909-8550
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China.
FAU - Zeng, Yongqing
AU  - Zeng Y
AUID- ORCID: 0000-0002-0740-2470
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Technology, Shandong Agricultural 
      University, Tai' an City, Shandong Province 271018, China. yqzeng@sdau.edu.cn.
LA  - eng
GR  - 2020LZGC012/Agricultural Animal Breeding Project of Shandong Province/China
GR  - SYL2017YSTD12/Shandong "Double Tops" Program/China
GR  - SDAIT-08-02/Shandong Modern Pig Technology & Industry System Project/China
GR  - ZR2018BC046/Shandong Provincial Natural Science Foundation/China
GR  - ZR2019MC053/Shandong Provincial Natural Science Foundation/China
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Receptors, Interferon)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Macrophages, Alveolar/metabolism/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*physiopathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Receptors, Interferon/*metabolism
MH  - Sus scrofa
MH  - Swine
PMC - PMC8170216
OTO - NOTNLM
OT  - IFNLR1
OT  - PRRSV
OT  - cell cycle
OT  - proliferation
OT  - replication
COIS- The authors declare no conflicts of interest.
EDAT- 2021/06/01 06:00
MHDA- 2021/09/14 06:00
PMCR- 2021/05/01
CRDT- 2021/05/31 06:42
PHST- 2021/02/04 00:00 [received]
PHST- 2021/04/11 00:00 [revised]
PHST- 2021/04/15 00:00 [accepted]
PHST- 2021/05/31 06:42 [entrez]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/09/14 06:00 [medline]
PHST- 2021/05/01 00:00 [pmc-release]
AID - 22.e39 [pii]
AID - 10.4142/jvs.2021.22.e39 [doi]
PST - ppublish
SO  - J Vet Sci. 2021 May;22(3):e39. doi: 10.4142/jvs.2021.22.e39.