PMID- 34057716
OWN - NLM
STAT- MEDLINE
DCOM- 20210810
LR  - 20231213
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2308
DP  - 2021
TI  - Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular 
      Compartment.
PG  - 83-94
LID - 10.1007/978-1-0716-1425-9_7 [doi]
AB  - Bone marrow stromal cells (BMSCs) account for an extremely small percentage of 
      total bone marrow cells; therefore, it is technically challenging to harvest a 
      good quantity of BMSCs with good viability using fluorescence-activated cell 
      sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for 
      flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines 
      facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental 
      characteristics of these diverse cell populations.
FAU - Matsushita, Yuki
AU  - Matsushita Y
AD  - University of Michigan School of Dentistry, Ann Arbor, MI, USA.
FAU - Ono, Wanida
AU  - Ono W
AD  - University of Michigan School of Dentistry, Ann Arbor, MI, USA.
FAU - Ono, Noriaki
AU  - Ono N
AD  - University of Michigan School of Dentistry, Ann Arbor, MI, USA. 
      noriono@umich.edu.
LA  - eng
GR  - R01 DE026666/DE/NIDCR NIH HHS/United States
GR  - R01 DE029181/DE/NIDCR NIH HHS/United States
GR  - R01 DE030630/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Culture Techniques
MH  - *Cell Lineage
MH  - Cell Proliferation
MH  - *Cell Separation
MH  - Cells, Cultured
MH  - Chemokine CXCL12/genetics/metabolism
MH  - *Flow Cytometry
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Luminescent Proteins/genetics/metabolism
MH  - Mesenchymal Stem Cells/metabolism/*physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - RNA-Seq
MH  - Red Fluorescent Protein
PMC - PMC8580286
MID - NIHMS1750255
OTO - NOTNLM
OT  - Bone marrow stromal cells (BMSCs)
OT  - C-X-C motif chemokine ligand 12 (CXCL12)
OT  - Fluorescence-activated cell sorting (FACS)
OT  - In vivo lineage-tracing experiments
OT  - Mesenchymal stem cells (MSCs)
OT  - Single cell RNA-seq
OT  - Skeletal stem cells (SSCs)
OT  - Transgenic reporter mouse lines
COIS- Declaration of interests: The authors declare no competing interests.
EDAT- 2021/06/01 06:00
MHDA- 2021/08/11 06:00
PMCR- 2021/11/10
CRDT- 2021/05/31 12:59
PHST- 2021/05/31 12:59 [entrez]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/08/11 06:00 [medline]
PHST- 2021/11/10 00:00 [pmc-release]
AID - 10.1007/978-1-0716-1425-9_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2308:83-94. doi: 10.1007/978-1-0716-1425-9_7.