PMID- 34058723
OWN - NLM
STAT- MEDLINE
DCOM- 20210615
LR  - 20210615
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 128
DP  - 2021 Aug
TI  - Gene expression profiles of mitochondria-endoplasmic reticulum tethering in human 
      gingival fibroblasts in response to periodontal pathogens.
PG  - 105173
LID - S0003-9969(21)00136-9 [pii]
LID - 10.1016/j.archoralbio.2021.105173 [doi]
AB  - OBJECTIVE: The current study aimed to elucidate the potential involvement of 
      mitochondria-endoplasmic reticulum contact genes in the pathogenesis of 
      periodontal disease by monitoring levels of contact associated genes including 
      Mitofusion 1 (MFN1) and MFN2, inositol 1,4,5-trisphosphate receptor (IP3R), 
      chaperone glucose-regulated protein 75 (GRP75), sigma non-opioid intracellular 
      receptor 1 (SIGMAR1) and phosphate and tensin homolog induced putative kinase 1 
      (PINK1) in human gingival fibroblasts in response to periodontal pathogens 
      Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. 
      gingivalis) in vitro. DESIGN: Primary human gingival fibroblasts were exposed to 
      live cultures of P. gingivalis (W83; ATCC BAA-308) and F. nucleatum (subsp. 
      Polymorphum; ATCC 10953) alone or in combination for 4 h at a 50 or 200 
      multiplicity of infection. Escherichia coli lipopolysaccharide (10 mug/mL) 
      exposure was used as a positive control. Gene expression levels of contact genes 
      (MFN1, MFN2, IP3R, GRP75, SIGMAR1 and PINK1) as well as a proinflammatory 
      cytokine, Tumor necrosis factor-alpha (TNF-alpha), and the apoptosis associated gene, 
      Immediate early response 3 (IER3), were evaluated by reverse transcription 
      polymerase chain reaction analysis. RESULTS: MFN1, GRP75, IP3R and PINK1 were 
      significantly upregulated by P. gingivalis with or without F. nucleatum. Only P. 
      gingivalis with F. nucleatum caused a significant upregulation of SIGMAR1. TNF-alpha 
      and IER3 gene expression positively correlated with the contact-associated gene 
      expression changes. CONCLUSION: F. nucleatum and P. gingivalis alone or in 
      combination may differentially dysregulate the gene expression levels of 
      contact-associated genes in human gingival fibroblasts. These host-microbiome 
      interactions may mechanistically be important in the pathogenesis of periodontal 
      disease.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Aral, Kubra
AU  - Aral K
AD  - School of Dentistry, University of Birmingham, Birmingham, UK; Department of 
      Periodontology, Faculty of Dentistry, Inonu University, Malatya, Turkey. 
      Electronic address: drkubraaral@gmail.com.
FAU - Milward, Michael R
AU  - Milward MR
AD  - School of Dentistry, University of Birmingham, Birmingham, UK.
FAU - Cooper, Paul R
AU  - Cooper PR
AD  - School of Dentistry, University of Birmingham, Birmingham, UK; Sir John Walsh 
      Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New 
      Zealand.
LA  - eng
PT  - Journal Article
DEP - 20210527
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
SB  - IM
MH  - Endoplasmic Reticulum
MH  - Fibroblasts
MH  - Fusobacterium nucleatum
MH  - *Gingiva
MH  - Humans
MH  - Mitochondria
MH  - Porphyromonas gingivalis
MH  - *Transcriptome
OTO - NOTNLM
OT  - Endoplasmic reticulum
OT  - Fusobacterium nucleatum
OT  - Gingival fibroblasts
OT  - Mitochondria
OT  - Periodontal disease
OT  - Porphyromonas gingivalis
EDAT- 2021/06/01 06:00
MHDA- 2021/06/16 06:00
CRDT- 2021/05/31 20:30
PHST- 2021/03/09 00:00 [received]
PHST- 2021/05/21 00:00 [revised]
PHST- 2021/05/24 00:00 [accepted]
PHST- 2021/06/01 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2021/05/31 20:30 [entrez]
AID - S0003-9969(21)00136-9 [pii]
AID - 10.1016/j.archoralbio.2021.105173 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2021 Aug;128:105173. doi: 10.1016/j.archoralbio.2021.105173. Epub 
      2021 May 27.